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벼의 자포니카형간 교배에서 유래된 F₂ 집단을 이용한 연관지도 작성 및 출수 관련 유전자의 QTL 분석
송문태(Moon Tae Song),이점호(Jeom Ho Lee),조윤상(Youn Sang Cho),정오영(O . Young Jeong),황흥구(Hung Goo Hwang) 한국유전학회 2001 Genes & Genomics Vol.23 No.4
Most of the molecular linkage maps in rice were constructed using segregating populations derived from crosses between parents with a wide genetic distance, such as japonica and indica rices, because of the high polymorphic profiles in them. However, the molecular markers in genetic maps in the populations of japonica/japonica or indica/indica are more useful in practice since these markers can be utilized directly in the concurrent breeding programs. This study aims to develop a genetic map using a population derived from early heading japonica cultivar Olbyeo1 and late heading cultivar Ilpumbyeo. Plant height and heading dates were checked in 300 F2 plants, from which randomly selected sixty plants were analyzed with DNA markers. One hundred and twenty nine polymorphic markers were detected in the segregating population and fifty markers were located in seven linkage groups. Total genetic distance of the seven linkage group was 795.5 cM with an average 17 cM distance between markers. Two QTL for heading days were detected in the linkage group 4 whose individual LOD values were 4.06 and 6.69, respectively. Genetic synteny of the seven linkage groups in the present study is being surveyed with the established rice linkage maps with more molecular markers.
Expression of two-cold regulated genes of canola responding to cold acclimation
Moon Tae Song(宋文台),로렌스코플랜드(Lawrence O. Copeland) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
Cold acclimation increases freezing tolerance and the changes of gene expression may involve in the enhancement of freezing tolerance during cold acclimation. This study was conducted to determine the responses of two cold-regulated genes in canola (Brassica napus L.), BN28 and BN115, to cold acclimation and find out correlation between increasing of freezing tolerance and the expression of the genes. Six winter cultivars and one spring cultivar that had previously exhibited a broad range of winter survival were examined in a controlled environment chamber. Southern blot analysis revealed that the seven cultivars examined had the same copy number of BN28 and BN115. Northern blot analysis indicated that these genes were induced within one day by cold temperature and turned off immediately by deacclimation. Though time-course increase of transcript in one of the genes was highly correlated with the time-course increase of freezing tolerance, no correlation was found at the cultivar lever at specific time of measurement. However, this does not rule out the possible role of cold-regulated genes in freezing tolerance of canola.