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손상수,Vishnu Revuri,Md. Nurunnabi,곽광수,이용규 한국고분자학회 2016 Macromolecular Research Vol.24 No.3
Biosynthetic hydrogels have proved to be a credible solution in developing cost effective scaffolds with superlative mechanical properties for biomedical applications. Elastic hydrogels have emerged with advanced application possibilities for cartilage tissue regeneration and cell implantation. However, a hydrogel scaffold that mimics the properties of biological tissues in terms of elasticity, provision of favorable environment for cell growth and biocompatibility are rarely reported. In this research, we developed photocrosslinked hyaluronic acid/pluronic F127 (HA/PF) porous hydrogels with exceptional mechanical and water sorption properties. In order to retain the micellar phase of PF in the hydrogels, we restrained their concentrations to 8 wt% in the hydrogel matrices. Further optimization such as duration of photocrosslinking resulted in hydrogel scaffolds with remarkable mechanical properties. Topical and cross-sectional scanning electron microscopy images depicted the dense interconnected porous networks within the hydrogel matrices. Rheology studies confirmed the importance of PF concentrations in obtaining the hydrogels with enhanced toughness and mechanical properties. The results from the microscopic rheology studies were further testified by applying macroscopic mechanical distortions over the hydrogels. Hydrogels with higher PF concentrations restrained the degradations and displayed enhanced mechanical properties. By retaining the micellar structures in the HA/PF hydrogel scaffolds, the polypropylene blocks in PF were able to reversibly fold and unfold to favor the energy dissipation during the mechanical deformation and aid in improving the mechanical properties of the hydrogels. The overall properties of the HA-PF hydrogels show optimum feasibility for hard tissue engineering application.
손상수(Sang-Su Son),지원대(Won-Dae Ji),정현채(Hyun-Chae Chung) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.4
매실을 사용하여 초산을 생산하기 위하여 알콜 농도, 발효 온도, 당 농도의 3변수와 5수준의 fractional factorial design에 의하여 RSM computer program을 사용하여 최적 발효조건을 조사하였다. 초산함량에 대한 회귀분석결과, R²는 0.9462로 높게 나타났다. 알콜 농도 8.76%, 발효 온도 26.27℃, 당농도 8.42%에서 발효하는 것이 초산 생성을 위하여 가장 양호하였으며, 이 조건에서의 초산 생성 예상치는 3.23%이었다. To produce acetic acid from Mume (Prunus mume Sieb. et Zucc) fruits, fermentation conditions were optimized by a response surface methodology (RSM) using the fractional factorial design with 3 variables and 5 levels. The coefficient of determination (R²) of response surface regression equation for acetic acid production was 0.9462. Optimum conditions for acetic acid production were involved with 8.76% of alcohol content, 26.27℃ of fermentation temperature and 8.42% of sugar content. Finally, predicted level of acetic acid production at these conditions was 3.23%.
손상수(Sang-Su Son),지원대(Won-Dae Ji),정현채(Hyun-Chae Chung) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.4
매실을 사용하여 알콜을 생산하기 위하여 당농도, 발효온도, 발효시간의 3변수와 5수준의 fractional factorial design으로 RSM computer program을 사용하여 최적 발효조건을 조사하였다. 알콜함량에 대한 회귀분석결과, R²는 0.9276으로 높게 나타났다. 당농도 8.39%, 발효온도 28.86℃에서 3.84일간 발효하는 것이 알코올 생성을 위하여 가장 양호하였으며, 이 조건에서의 알코올 생성 예상치는 5.24%이었다. To produce alcohol from Mume (Prunus mume Sieb. et Zucc) fruits, fermentation conditions were optimized by a response surface methodology (RSM) using the fractional factorial design with 3 variables and 5 levels. The coefficient of determination (R²) of response surface regression equation for alcohol production were 0.9276. Optimum conditions for alcohol production were involved with 8.39% of sugar content, 28.86℃ of fermentation temperature and 3.84 days of fermentation time. Finally, predicted level of alcohol production at these conditions was 5.24%.