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국·내외 도시캐릭터의 성공 사례를 통해 본 서울시 캐릭터 ‘해치’의 매니지먼트 연구
서민경(Min-Kyoung Seo),고정민(Jeong-Min Ko) 한국애니메이션학회 2021 애니메이션연구 Vol.17 No.4
본 연구의 목적은 캐릭터 매니지먼트 관점에서 캐릭터를 성공적으로 활용한 사례를 통해 성공요소를 추출하고, 서울시 해치캐릭터에 대한 문제점 개선과 향후 매니지먼트 방안을 제안하는 것이다. 연구 결과, ① 커뮤니케이션, ②장소성, ③스토리, ④의인화, ⑤공익성, ⑥OSMU, ⑦혁신성의 7가지 성공요소가 추출되었고, 해치는 활발한 커뮤니케이션을 위해 시민들과 친해져야 하고, 활동적인 테마공간이 필요하며, 콘텐츠 다양성을 위해 스토리를 업그레이드해야 된다는 결과가 도출되었다. 또한 차별화된 콘텐츠를 만들기 위해 별도의 전문적인 매니지먼트 조직을 구성할 필요가 있다는 점을 제안하였다. 본 연구는 커뮤니케이션, 디자인, 마케팅 분야로 각 개별적으로 연구된 선행연구를 종합적으로 정리, 총체적인 개념인 캐릭터 매니지먼트에 대한 연구의 첫 시작이라 볼 수 있고, 실무적으로는 캐릭터분야의 실무자들에게 아이디어를 정리하기 위한 틀을 정립하는 데 도움이 될 것이다. The purpose of this study is to study the method for character management that performs integrated management, to analyze the cases of successful use of characters and to extract success factors in detail, and to improve the problems of Haechi characters in Seoul and in the future. Also, we would like to propose a management plan. As a result of the study, success factors of ① communication, ② place, ③ story, ④ personification, ⑤ public interest, ⑥ OSMU, and ⑦ innovation were extracted. We were able to summarize that the story has to be upgraded and to create and manage a professional management organization to create such differentiated content. This study was the first start of research on character management, which is a general concept, by comprehensively organizing prior research that was individually studied in the fields of communication, design, and marketing. It will help you establish a framework of character management.
토양에서 분리한 Bacillus Siamensis BC-S09의 항진균 활성
서민경 ( Min-kyoung Seo ),양현주 ( Hyun-joo Yang ),남효송 ( Hyo-song Nam ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-
A bacterium having antifungal activity was isolated from soil in Gokseong-gun, South Korea and identified as Bacillus siamensis. Antifungal bacteria were isolated from various soil samples. The samples were suspended in phosphate-buffered saline (PBS), spread on Tryptic Soy agar (TSA) medium and allowed to grow at 30℃ for 24 h under aerobic condition. The strain BC-S09 exhibiting the highest antifungal activity was identified as Bacillus siamensis. The strain BC-S09 was identified by 16S rRNA gene sequence analysis after polymerase chain reaction (PCR) amplification using the universal primers 27F and 1492R as described Stackebrandt and Goodfellow. The 16S RNA sequences were compared using “16S-based ID” in the EzBioCloud (https://www.ezbiocloud.net) database. The phylogenetic analysis was performed using the neighbor-joining method in the MEGA program (https://www. megasoftware.net/). Various fungi were kept on potato-dextrose agar (PDA) slants at 25℃. Spore inoculums were prepared by growing the molds on 10-days-old PDA slants and collected after vigorously shaking slants with sterile peptone water (0.2% w/v). Bacterial isolates exhibiting antifungal activity were observed directly on PDA agar plates where the indicator molds test microorganisms were placed with control and experimental group. Bacteria and fungi was incubation of 25℃ for 2 day to 7 day in aerobic incubator. The results showed that Bacillus siamensis BC-S09 suppressed the growth of phytopathogenic fungi including Colletotrichum coccodes (KACC40010), Colletotrichum acutatum (KACC40689), Colletotrichum acutatum (KACC40804 - strong virulence), Altermaria citri (KACC42128), Fusarium oxysporum, Rhizoctonia solani.
조일규 ( Il Kyu Cho ),서민경 ( Min-kyoung Seo ),양현주 ( Hyun-joo Yang ),장소영 ( So-young Jang ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-
왕우렁이(golden apple snails)는 1983년 식용목적으로 국내에 도입되었다가 1992년부터 왕우렁이의 섭식 특성을 활용하여 벼농사의 논 잡초 방제용으로 사용되기 시작하였다. 국내에 유입된 종은 Pomacea canaliculata 로 남아메리카가 원산지이며 국내에 서식하고 있는 토종우렁이와는 외형, 먹이, 번식 등에서 확연한 차이가 있다. 토종우렁이가 새끼로 번식하는 것에 비해 알로 번식하는 왕우렁이는 원산지인 열대지방에서는 왕우렁이가 벼의 해충으로 인식되고 있고, 필리핀, 대만 등 동남아시아 및 일본에서 벼농사의 심각한 피해를 주기 때문에 IUCN(세계자연보전 연맹)에서는 세계 100대 침입 외래종으로 분류하고 있다. 기후 온난화로 인해 국내에서도 왕우렁이가 월동을 하게 되면서 남부지역에 피해를 끼침으로 환경부는 2007년 왕우렁이를 법정 생태계 위해 생물 2등급으로 분류하고 있다. 본 시험에서는 왕우렁이 월동 방지를 위하여 친환경농업자재 5가지 제품(제품 1: 석회 유황, 제품 2: 황토 및 유황, 제품 3: 제충국 함유, 제품 4: 마트린 함유 제품 5: Pseudomonas chlorohaphis, O6)을 1000배, 2000배로 각각 희석하여 처리 후 시간대별로 활동성을 확인하였다. 24시간 이후 왕우렁이의 폐사 여부를 확인하여 방제률을 확인하였는데, 제품 3이 가장 우수한 왕우렁이 방제 효과를 보였으며, 그 다음으로 제품 1, 2, 4가 유사한 방제력을 나타냈으나 제품 5는 방제 효과가 없는 것으로 나타났는데 이는 Pseudomonas chlorohaphis가 왕우렁이 방제에 영향을 주지 않는 것으로 판단된다.
양현주 ( Hyun-joo Yang ),서민경 ( Min-kyoung Seo ),최제연 ( Je Yeon Choi ),남효송 ( Hyo-song Nam ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-
Xanthomonas oryzae pv. oryzae (KACC10208) was the causative agent of bacterial blight in rice. Bacillus velezensis has antimicrobial activity to Xanthomonas oryzae pv. oryzae (KACC10208) and antifungal activities Colletotrichum coccodes (KACC40010), Colletotrichum acutatum (KACC40689). The purpose of this study was to develop an economical culture medium for the optimal cell growth and spore forming by submerged fermentation of B. velezensis. B. velezensis was grown in Lysogeny broth(LB) or in Medium Optimal for Antibacterial activity(MOA) at 30℃ and 150 rpm. Antifungal activity were observed directly on PDA agar plates where the indicator molds test microorganisms were placed with control and experimental group. The incubation condition was 25℃ for 48 h to 72 h in aerobic incubator. Experiments were performed at three tines with biological replicates. The data were analyzed using one-way analysis of variance(ANOVA) followed by Duncan’s multiple range test (P<0.05). The antimicrobial activity of the cell-free supernatant of Bacillus velezensis strain grown on each carbon and nitrogen source against the phytopathogenic Xanthomonas oryzae pv. oryzae (KACC10208) was tested on TSA plates using the disc diffusion method. The optimal carbon and nitrogen sources were determined as sucrose and soy bean flour, respectively. Additionally, MgSO4 was significantly involved in the growth and sporulation of Bacillus velezensis in addition to carbon and nitrogen sources. The results showed that bacterial cells of 1.0 x 109cfu/m and sporulation yield of 90%.
벼 세균성 흰잎마름병(Xanthomonas Oryzae pv. Oryzae)의 생물학적 방제를 위한 미생물 사용방법
정종국 ( Jong-kook Jung ),서민경 ( Min-kyoung Seo ),양현주 ( Hyun-joo Yang ),구헌우 ( Heon-woo Ku ),남효송 ( Hyo-song Nam ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-
The purpose of this study is to develop eco-friendly microbial agents and application technologies for the safe production of rise by control of rice Bacterial Blight (Xanthomonas oryzae pv. oryzae). Rice seeds (Oryza sativa cv. ShinDongjin) were inoculated with 3 types of microorganisms (Bacillus amyloliquefaciens KB3, Bacillus velezensis LM11, Serratia spp IM2), chemical disinfectant (tebuconazole 12.5 + prochloraz copper chloride 12.5%) and Trichoderma at 25°C for 24 hours. After inoculation, the rice seeds were washed twice with distilled water, placed on PDA medium, and incubated at 25°C for 48 hours. As a result of the culture, microorganisms causing white leaf blight were cultured in the rice seed treated with chemical disinfectant, and each strain was cultured in the rice seed inoculated with Bacillus amyloliquefaciens KB3, Bacillus velezensis LM11, and Trichoderma. The germination rate of the seed inoculated with each sample was confirmed. In the case of rice seeds treated with microorganisms and Trichoderma, the germination rate was about 3.5 times higher than that of chemical disinfectants. As a result of checking the growth level of each sample 2 weeks after sowing, it was confirmed that the Bacillus velezensis LM11 inoculated sample had a higher growth rate than other samples. Each sample was transplanted into a Wagner pot and growth was investigated 2 weeks after transplantation. As a result, the sample inoculated with Bacillus velezensis LM11 showed a higher growth rate than the other samples, and the rest of the samples grew similarly. Each rice seed was immersed in the sample to infect the grown rice with Xanthomonas oryzae pv. oryzae, which causes white leaf blight. As a result of infection, it was confirmed that the infection rate was the lowest in Bacillus velezensis LM11.
송형근 ( Hyeong-geun Song ),조유성 ( Yu-sung Cho ),양현주 ( Hyun-joo Yang ),서민경 ( Min-kyoung Seo ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
It is well known that the rhizosphere microbial communities are closely associated with plant roots. The rhizosphere microbiota can be harmful or beneficial to the host plants. In this study, to know whether pathogen-caused plant diseases would lead changes of rhizosphere microbiota or not, pathogenic bacteria were added to the plants through leaf stomata and the soils to induce the diseases and the rhizosphere microbial communities were analyzed using multiplex sequencing. The plant pathogen Xanthomonas euvesicatoria 173-1 causing bacterial spots was applied to Korean chili peppers (Capsicum annuum) and the plant pathogen Erwinia carotovora SCC1 causing bacterial soft rot was applied to Chinese cabbage (Brassica rapa). The pot soils were collected before and after the pathogen applications and disease occurrences and analyzed for v4 region of bacterial 16S rRNA gene using Illumina iSeq 100 system. In the bacterial spot disease peppers, soil bacterial diversity was larger than in the normal pepper soils in terms of both species richness and evenness. Although it was not easy to indicate the responsible taxa for the differentiation, the communities were found to have diverged in both the cases of the control and disease plants, supported by NMDS and PCoA analyses on distance metrics of the communities. From the Chinese cabbage soils, there was less differentiation of microbiota between the normal and disease plants, than in the pepper soils. In the further study, it would be also interesting to investigate the important factors for divergence of results between pepper and cabbage.