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Aloe saponaria 캘러스의 열수 추출물 유래 다당의 특성
백진홍(Jin-Hong Baek),김명욱(Myung-Uk Kim),강태수(Tae-Su Kang),허원(Won Hur),이신영(Shin-Young Lee) 한국생물공학회 2009 KSBB Journal Vol.24 No.1
The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 μM NAA (α-naphthalene acetic acid) and 3-7 μM kinetin under incubation in the dark at 25℃ for 6 weeks. The hot water extract (100℃, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.
알로에 베라 겔 가공부산물로서의 섬유질 분획의 성분 및 물리화학적 특성
백진홍,이신영 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.2
The fibrous material fraction as a by-product from the commercial aloe vera gel processing was obtained and freeze dried. The physicochemical characteristics such as the proximate composition, crystalline/surface structures and several physical functionalities including the water holding capacity (WHC), swelling capacity (SW), oil holding capacity (OHC), emulsion/foam properties and viscosity properties of this powdered sample (100 mesh) were investigated and analyzed by comparison with commercial α-cellulose as a reference sample. The total dietary fiber content of powdered sample was very high as much as 87.5%, and the insoluble dietary and soluble dietary fiber content ratios were 77.6 and 22.4%, respectively. The FT-IR spectrum of powdered sample showed a typical polysaccharide property and exhibited a x-ray diffraction pattern for cellulose III and IV like structure. SW (8.24±0.15 mL/g), WHC(6.40±0.19 g water/g solid) and OHC(10.32±0.29 g oil/g solid) of freeze dried aloe cellulose were about 3.3, 1.4 and 2 times higher than those of commercial α-cellulose, respectively. Aloe cellulose (~2%, w/v) alone had no foam capacity while improved the foam stability of protein solution (1% albumin+0.5% CaCl2) by factor of 300%. Emulsion capacity of 2%(w/v) aloe cellulose was about 70% level of 0.5%(w/v) xanthan gum, but its emulsion stability was about 1.2 times higher than that of xanthan gum. Also, aloe cellulose containing CMC (carboxyl methyl cellulose) of 0.3%(w/v) showed a very good dispersity. Aloe cellulose dispersion of above 1%(w/v) exhibited higher pseudoplasticity and concentration dependence than those of α-cellulose dispersion, indicating the viscosity properties for new potential usage such as an excellent thickening agent.
알로에 베라 유래 식이섬유의 In Vitro 및 In Vivo 생리기능 특성
백진홍,차태양,허진철,이상한,이신영 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.2
A fiber fraction (Aloe cellulose), the by-product obtained from Aloe vera gel processing was freeze dried and investigated for in vitro glucose/ bile acid retarding effects of powdered sample (100 mesh) comparing with commercial α-cellulose as a reference sample. We also examined the effectiveness of physiological functionality such as the antiobesity and anti-constipation on Sprague-Dawley (SD) rat. The Aloe cellulose powders during in vitro dialysis experiment for 2 hours exhibited the glucose and bile acid retarding index of 20.32-35.2% and 53.13-28.30%, respectively. Especially, freeze dried aloe cellulose showed the 2.5 and 1.2-6 times higher effect on in vitro glucose and bile acid retardation than those of α-cellulose. These relatively good retarding effects on glucose and bile acid diffusion suggest a potential of preventing from diabetes and arteriosclerosis of some extent. Also, the results from animal experiments on SD rats fed a high-fat diet for 4 weeks suggested that Aloe cellulose might be used as a novel dietary fiber showing an effective anti-obesity and anti-constipation effect.
백진홍 ( Jin Hong Beak ),강은원 ( Eun Won Kang ),김성아 ( Sung A Kim ),차지민 ( Ji Min Cha ),이신영 ( Shin Young Lee ) 한국산업식품공학회 2013 산업 식품공학 Vol.17 No.4
Two fractions (permeate and retentate) from fiber free Aloe vera gel were prepared using an ultra-filtration (UF) system with a tubular ceramic membrane (MWCO of 50 kDa), and their dilute solution properties were investigated. The intrinsic viscosities of fiber free Aloe vera gel and the retentate and permeate fractions that were prepared by ultra-filtration were 18.48, 37.74, and 2.01 dL/g, respectively. UF aloe retentate as a polysaccharide rich fraction exhibited polyelectrolyte behavior showing a sharp decrease of intrinsic viscosity by the addition of NaCl. However, as its NaCl concentration increasing to 1 M, its intrinsic viscosity slightly increased due to conformational change caused by the high concentration of NaCl. On the one hand, the intrinsic viscosities of the dilute UF-retentate solution were exponentially decreased with increasing temperature, and the temperature dependency of its intrinsic viscosity showed a sharp discontinuity at 30℃ instead of a linear Arrhenius behaviour, indicating a variation of flow mechanism or network rearrangement of a molecular chain. The chain stiffness of polysaccharide rich fraction at bellow and above 0.5 M of NaCl addition was 0.048 and 0.515, respectively, showing different stiffenings and significant conformational change. The coil overlap parameter and critical concentration of UF-retentate were 3.0 and 0.08 g/dL, respectively.
알로에 베라 겔 가공부산물로서의 섬유질 분획의 성분 및 물리화학적 특성
백진홍 ( Jin Hong Baek ),이신영 ( Shin Young Lee ) 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.2
The fibrous material fraction as a by-product from the commercial aloe vera gel processing was obtained and freeze dried. The physicochemical characteristics such as the proximate composition, crystalline/surface structures and several physical functionalities including the water holding capacity (WHC), swelling capacity (SW), oil holding capacity (OHC), emulsion/foam properties and viscosity properties of this powdered sample (100 mesh) were investigated and analyzed by comparison with commercial α-cellulose as a reference sample. The total dietary fiber content of powdered sample was very high as much as 87.5%, and the insoluble dietary and soluble dietary fiber content ratios were 77.6 and 22.4%, respectively. The FT-IR spectrum of powdered sample showed a typical polysaccharide property and exhibited a x-ray diffraction pattern for cellulose Ⅲ and IV like structure. SW (8.24±0.15mL/g), WHC(6.40±0.19g water/g solid) and OHC(10.32±0.29g oil/g solid) of freeze dried aloe cellulose were about 3.3, 1.4 and 2 times higher than those of commercial α-cellulose, respectively. Aloe cellulose (~2%, w/v) alone had no foam capacity while improved the foam stability of protein solution (1% albumin+0.5% CaCl2) by factor of 300%. Emulsion capacity of 2%(w/v) aloe cellulose was about 70% level of 0.5%(w/v) xanthan gum, but its emulsion stability was about 1.2 times higher than that of xanthan gum. Also, aloe cellulose containing CMC (carboxyl methyl cellulose) of 0.3%(w/v) showed a very good dispersity. Aloe cellulose dispersion of above 1%(w/v) exhibited higher pseudoplasticity and concentration dependence than those of α-cellulose dispersion, indicating the viscosity properties for new potential usage such as an excellent thickening agent.