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파쇄화분 배지에서 성장한 Lactobacillus plantarum R 의 항균물질 생산에 관한 연구
김동신(D . S . Kim),최상훈(S . H . Choi),박상교(S . K . Park) 한국낙농식품응용생물학회 1998 한국유가공기술과학회지 Vol.16 No.1
N/A The object of this study was to confirm and purify antibacterial agents from the fermented cracked pollen with Lactobacillus plantarum R. The antibacterial activity according to the level of the concentration of the fermented cracked pollen showed the highest(22.7 ㎜) inhibition zone at 30% concentration. The cracked pollen neutralized with NaOH presented higher antibacterial activity than that of the non-neutralized one. The fermented cracked pollen added with the seven chemicals showed higher inhibition zone than that of the fermented cracked pollen without the chemicals. Lb. plantarum R produced more effective agents in the cracked pollen medium than the 11% total solid milk medium. When the fermented cracked pollen was treated with ammonium sulfate and centrifuged, the antibacterial activity remained in the supernatant. The extraction procedures for the antibacterial agents from the fermented cracked pollen using methanol-acetone and ethyl acetate showed a better effect with ethyl acetate. The fractions having the antibacterial activity from Sephadex G-50 gel chromatography were applied on the TLC and two bands were detected on the plate under UV light. Two bands didn`t showed any antibacterial activity but the area between two bands did.