리그닌의 분해가 우수한 Streptomyces halstedii ssp. scabies SA1-27과 Streptomyces violaceusinger C1-6에서 생성되는 효소들에 관한 연구

김태전 ( Tai Jeon Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.2

This study was done to know a kind and change (transition) of enzymes produceed by Streptomyces halstedii ssp. scabies SA1-27 and Streptomyces violaceusinger C1-6 which showed good lignolytic activity and a good decolorization ratio of remazol brilliant blue R(RBBR) dye. These strains were isolated from soil and identified by the author. The basal medium containg 0.2% glucose was used to measure enzyme activity, Lignin peroxidase 1 (Lip 1) was measured by the methods of Choi, and Bourbonnais and Paice. Lignin peroxidase 2 (Lip 2) was measured by the methods of Ishida et al and Ramachandra et al using 2.4-dichlorophenol(2.4·DCP), manganese peroxidase(Mnp), veratryl alcohol oxidase (VAO), and laccase. They were measured by each of the methods of Choi and Paszczynski et al, and Bourbonnais and Paice, and De Jong et al. In the results, the kind of enzymes produced by Streptomyces halstedii ssp. scabies SA1-27 were Lip 1, Lip 2, VAO, and laccase, and their activities indicated the highest value as each 4.95 nmol/mg protein, 8.45(×100-3) unit, 10.25 nmol/mg protein, 9.20 nmol/mg protein on the sixth day of the culture and decreased gradually over time. The kind of enzymes produced by Streptomyces violaceusinger C1-6 were Lip 1, Lip 2, Mnp, VAO, and laccase, and their activities indicated the highest value as each 4.90 nmol/mg protein, 13.85(×100-3) unit, 3.10 nmol/mg protein, 11.30 nmol/mg protein, 4.45 nmol/mg protein on the sixth day of the culture and decreased gradually over time. Consequently, the author knew the fact that there were few differences in the kind and quantity of enzymes produced by the two Streptomyces strains, but all enzyme activities indicated the highest value on the sixth day of the culture and decreased gradually over time.

두 Streptonmyces Strains에 의한 다핵 방향족 탄화수소화합물의 분해

김태전 ( Tai Jeon Kim ),김승곤 ( Sung Kon Kim ),배형준 ( Hyung Joon Bae ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

Tle purpose of this study was to investigate the degradation abilities of polynuclear aromatic hydrocarbon (PAHs; anthracene, phenanthrene, benzo(a)pyrene) compounds using Streptomyces halstediiscabies SAI-36, Streptomyces avendulas SA2-14, and Streptomyces badius (ATCC 39117, con-trol group). Benzo(a)pyrene and anthracene showed a the degradation ability of 99.5% and 99.2%respectively by Streptomyces lavendulas SA2-14. Benzo(a)pyrene showed the degradation ability of 98.0% by Streptomyces halstedii scabies SAl-36. Anthracene showed the lowest degradation ability of 47.7% by Streptomyces badius(ATCC 39117). Benzo(a)pyrene and phenanthren showed the hi항lest degradation abilities of 71.7% and 78.8% respectively. The resu1ts suggested that two experimental strains denionstrated superior abilities above the control group in degradation of P AHs compounds and Streptomyces lavendulas SA2-14 was the superior strain between the two experimental strains. The results also proposed the possibility of the use of actinomycetes in the degradation of non-degradable materials. These resu1ts were consistent with previous research results that Streptomyces lavendulas SA2-14 was the best strain in degradation ability of lignin, decoloration abilities of different dyes, different enzyme production abilities and degradation abilities of phenol compounds. It is suggested that more basic research is needed for actinomy-cetes to be used as a biological degradation.

갑상선의 정상과 여포상 종양 조직의 은 핵소체 형성부위 (AgNOR)와 증식세포 핵 항원 (PCNA)에 관한 조사

김태전 ( Tai Jeon Kim ),한경희 ( Kyung Hee Han ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

The histologic or cytologic distinctions between follicular adenoma and carcinoma in thyroid tissue are sometimes difficult. For the diagnosis of follicular carcinoma, invasion of blood vesseles or capsules are considered defmitive criteria. However, the distinction of follicular adenoma from carcinoma may be difficult when these features are absent. Furthermore, in case with fme needle aspiration biopsy specimen, the distinction between follicular adenoma and carcinoma may also be difficult, as both are characterized by follicular form cells. The authors performed the sliver nucleolar organizer region(AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen(PCNA) on 5 samples of normal thyroid tissue, 8 follicular adenomas, and 10 carcinomas to evaluate whether these techniques can aid in these differential diagnosis. Significant differences were found in the mean AgNOR count between normal thyroid tissue (1.29 :t O.65) and follicular adenomas (2.88 :t 1.33) (P<O.α)()1) , but not between follicular adenomas and carcinomas(2.71 :t 1. 14). In the normal thyroid tissue, AgNORs were smooth and round ; in the follicular adenomas and carcinomas, however, they varied in size and shape. Compound AgNORs were commonly present in the tumors but were absent in normals. Antibody to PCNA did not stain normal thyroid tissue cells but stained many follicular adenomas and carcinoma cells. These results suggested that the AgNOR technique and PCNA immunohistochemistry could be used to distinguish normal thyroid tissue form follicular adenoma, but not distinguish follicular adenorna from carcinoma.

임상검체에서 분리된 Serratia sp. 2000-1에 의한 Protease의 생산 및 효소학적 성질

김태전(Tai Jeon Kim),김승곤(Sung Kon Kim),김상택(Sang Taek Kim) 대한의생명과학회 2000 Biomedical Science Letters Vol.6 No.3

본 논문은 환자들의 병소에서 채취된 가검물에 존재하는 미생물들 가운데 protease을 분비하는 균주들을 분리하고, 그 가운데 가장 효소생산력이 우수한 균주를 선정하여 가정용 Protease 세제생산에 활용 가능성을 알아보고자 그들이 생산하는 protease의 기초적 생산조건 및 부분적 효소학적 특성 등에 관한 실험에서 얻은 결과들을 보고하고자 작성되었다. Serratia sp. 2000-1로 동정된 본 실험 균주가 생산하는 protease의 기초적 생산조건과 부분적 효소학적 특성을 조사한 결과 효소생산을 위한 최적 배지에 탄소원과 질소원 그리고 금속염의 종류와 농도는 각각 Glucose 1.5%, C.S.P 2.0%, CaCl₂ 0.1%였다. 그리고 최적 배양온도는 30℃, 초발 pH는 8.0, 배양시간은 72시간 이었다. Protease의 정제는 ammonium sulfate precipitation, DEAE-cellulose, Sephadex G-200 column을 통하여 분리 정제하였으며 이때 최종 효소수율은 14.4%, 효소 비활성도는 약 29배 증가한 것으로 나타났다, 그리고 효소 작용의 최적온도와 pH는 35℃와 pH 8.0으로 나타났고, 효소 작용의 열과 pH에 대한 안정성은 40℃와 pH 6~10까지는 효소의 활성이 비교적 안정한 것으로 나타났다. 금속이온들 중 Mg²?, Ba²?, Ca²?, Mn²?은 효소활성을 촉진하나 Hg²?, Ag²?, Cu²?는 효소활성을 저해하는 것으로 나타났다. 그리고 효소활성 저해제들 중에는 SDS가 가장 강하게 효소활성을 저해하는 것으로 나타났다. The purpose of this study was to investigate the practical availability of protease production that can be used at home after isolating Serratia sp. 2000-1 which produced extracellular protease from clinical specimen. Basic production conditions and partial enzymatic characteristics of protease produced by Serratia sp. 2000-1 was as follows: The kind and concentration of carbohydrate, nitrogen and metal salts for optimal enzyme production condition were each identified as the concentration of 1.5% glucose, 2.0% CSP, and 0.1% CaCl₂, and the optimal temperature, time and initial pH for culture were each 30℃, 72 hours, and pH 8.0. The final enzymatic yeild that was purified by 3 steps with ammonium sulfate precipitation (45~80%), DEAE-cellulose column chromatography, and Sephadex G-200 gel chromatography was 14.4%, and enzyme inactivity rate increased approximately 29 folds. The optimal temperature and pH for purified protease activity were 35℃ and pH 7.0~8.0, and purified protease activity was relatively stable by 40℃ at pH 6~10 for 30 min, however heating at 60℃ for 30 min, it liminated detectable protease activity. The protease activity was activated by Mg²?, Ba²?, Ca²?, Mn²?, but inactiviaed by Hg²?, Ag?, Cu²?, and the protease activity was inhibited strongly by SDS among enzyme activity inhibitors. Further study is required to evaluate the practical availability of protease production that can be used at home by isolating Serratia sp. from more clinical specimen and examining protease more in details.

자궁경부에 전이된 반지세포암종 -1례 보고-

김태전 ( Tai Jeon Kim ),김성철 ( Sung Chul Kim ),한경희 ( Kyung Hee Han ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.2

This study is a report about a specific patient whose primary stomach adenocarcinoma metastasized to uterine cervix adenocarcinoma. A thirty-nine year old female patient was initially diagnosed as having metastatic adenocarcinoma in the supraclavicular lymph node. Upon further examination, she was diagnosed with stomach adenocarcinoma. 8 months later, a cervix punch biopsy was performed. The stains used for examination were H&E stain, PAS stain, Alcian blue stain, Mucicarmine stain, Papanicolaou’s (Pap.) stain, and as immunohistochemical stains, cytokeratin 7 and 20 were done. In the H&E stain, the tumor cells showed prominent and eccentric nuclei, thin nuclear membrane in abundant mucous cytoplasm, and cylinder shape. In the PAS stain, intracytoplasmic mucin vacuoles were stained with pink, and in Alcian blue and Mucicarmine stains, intracytoplasmic mucin vacuoles were stained with blue and red. As in the above results, she was diagnosed with undifferentiated adenocarcinoma. As found on the cytologic smear preparation of the uterine cervix stained by Papanicolaou``s stains, the background was relatively clear, the number of malignant cells was relatively low, and large and eccentric nuclei in abundant cytoplasm were observed. Upon observing the tissue preparation of the uterine cervix biopsy by H&E stain, a clear background, large and eccentric nuclei, and a signet ring cell types were observed, and the number of malignant cells were fewer than in the primary uterine cervix adenocarcinoma. The vacuoles in cytoplasm were observed. The nuclear membrane and chromatin were thick and very rough, and upon observation by cytokeratin 7 and 20 of immunohistochemical stain, the tumor cells indicated a positive rate of 70% and 20%, respectively. According to these results, also she was diagnosed with metastasized uterine cervix adenocarcinoma. In summary of the results of pathologic findings on stomach biopsy and cytologic, histopathologic, and immunohistochemical finding on uterine cervix biopsy, the adenocarcinoma of her uterine cervix could assert the adenocarcinoma of signet ring cell type that was metastasized from the primary undifferentiated adenocarcinoma in stomach.

유방암의 악성도에 따른 스테로이드호르몬 수용체와 종양단백질의 발현과의 상관성

김태전 ( Tai Jeon Kim ),문희주 ( Hi Joo Moon ),배형준 ( Hyung Joon Bae ),백운철 ( Oun Chul Back ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.1

This study was designed to compare and analyze the overall relationship between independent factors inducing breast cancer, the steroid hormone receptors(ER and PR), and oncoproteins(C-erbB-2, P-53 and Bcl-2). A total of 21 test samples underwent tissue studies with H&E staining to test histologic grading. ER, PR, P-53, C-erbB-2, Bcl-2, and Ki 67 were examined with immunohistochemistry. Although ER and PR showed no statistically significant difference in histologic grade, the induction of PR increased when induction of ER increased showing a significant relationship(P<0.05). C-erbB-2 oncoprotein showed no statistically significant relationship with histologic grade, nuclei mitosis index, lymphnode metastasis, menopause, and P-53 oncoprotein, whereas it showed a statistically significant relationship with PR, ER, and Bcl-2 oncoproteins(P<0.05). P-53 oncoprotein showed no statistically significant relationship with C-erbB-2 oncoprotein, histologic grade, lymphnode metastasis, and menopause, whereas it showed statistically significant relationships with ER, PR, Bcl-2, and nuclei mitosis index(P<0.05). Bcl-2 oncoprotein showed no statistically significant relationship with lymphnode metastasis, histologic grade, and menopause, whereas it showed statistically significant relationships with other pathological prognosis factors(ER, PR, P-53, C-erbB-2 and nuclei mitosis index)(P<0.05). In conclusion, ER, PR, and oncoproteins(C-erbB-2, P-53 and Bcl-2) showed no significant difference with histologic grade. However ER, PR, and oncoproteins showed significant relationships with each other. The induction rate of P-53 oncoprotein was low when that of Bcl-2 oncoprotein was high while Bcl-2 oncoprotein and P-53 oncoprotein showed a reciprocal relationship with each other.

갑상선에서 세침흡입생검에 의한 세포학적 진단분석

김태전 ( Tai Jeon Kim ) 대한임상검사과학회 1994 대한임상검사과학회지(KJCLS) Vol.26 No.1

골조직의 신속한 진단을 위한 탈회방법의 비교 연구

김성철 ( Sung Chul Kim ),백운철 ( Oun Chul Back ),김태전 ( Tai Jeon Kim ),배형준 ( Hyung Joon Bae ),강희규 ( Hee Gyoo Kang ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1

These studies were done to know decalcification methods to reduce the time of decalcification for quick bone tissue diagnosis. When bone tissue was decalcified with 10 % formic acid at room temperature, decalcification and hematoxylin & eosin (H&E) stains were complete and satisfactory after 12 hours, but some of the tissue sections fell off during staining. In this way, decalcification, H&E stains were complete and satisfactory after 24 hours, 36 hours and 48 hours, tissue sections didn``t fall off during staining. When bone tissue was decalcified with 10 % formic acid in a 60 ℃ paraffin oven, decalcification and H&E stains were complete and satisfactory after 6 hours, but some tissue sections fell off during staining. In this way, decalcification and tissue sections were complete, with no falling off during staining after 8 hours, 10 hours, 12 hours, 14 hours, 24 hours, or H&E stains were satisfactory from 8 hours to 12 hours, but H&E stains appeared to reddish nucleus after 14 hours and 24 hours. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at DECAL machine frequencies of 15 Hz and 45 Hz, and for 6 hours, 12 hours and 24 hours at a DECAL machine frequency of 90 Hz. Decalcification and H&E stains were complete and satisfactory after 6 hours at the 15 Hz and 45 Hz DECAL settings. Some of the tissue sections fell off during staining at the 15 Hz DECAL machine setting. At the 90 Hz setting, decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 4 hours. In this way, decalcification, H&E stains, and tissue section were complete and satisfactory with no falling off during staining after 12 hours, 24 hours at all machine settings. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at 37 ℃ 3 hours, 6 hours and 12 hours at 45 ℃ and 1 hours, 5 hours and 10 hours at 60 ℃ with the RHS-1 machine setting at 60Hz. At the temperatures of 37 ℃, 45 ℃, and 60 ℃ decalcification, H&E stains, and tissue sections were complete and satisfactory, with no falling off during staining except for after 6 hours at 37 ℃. 3 hours, 1 hours, or decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 12 hours and 24 hours at 37 ℃, 6 hours and 12 hours at 45 ℃, and 5 hours at 60 ℃. But H&E stains appeared to reddish nucleus after 10 hours at 60 ℃. From the above reults, the authors were able to deduce that decalcification is accelerated by heat and frequency. We therefore think that it is necessary for machines which are similar to the RHS-1 machine to be maintained at the temperature evenly with agitation effect for quick decalcification.

염색체의 분석방볍과 세포유전학적인 검토

최수경 ( Soo Kyung Choi ),김태전 ( Tai Jeon Kim ) 대한임상검사과학회 1985 대한임상검사과학회지(KJCLS) Vol.17 No.1

Presented in this paper is the cytogenetic study in patients for suspected chromosomal abnormalities. karyotypes were obtained using short-term peripheral blood culture and conventional staining and G-banding methods for correct analysis of structural abnormalities. As a res ults, we confirmed structural abnomalities of chromsome by the G-banding study. it was not possible by the conventional staining. Therefore the obtained successful results by trypsin-Giemsa banding were preferable to the other methods.

石村湖의 營養醒類및 水質의 動態에 관한 調査硏究

崔漢榮(Han Young Choi),金泰田(Tai Jeon Kim),朴聖培(Sung Bae Park) 한국환경보건학회 1988 한국환경보건학회지 Vol.14 No.1

This study was performed to investigate a seasonal and vertical variation of the nutrients, water temperature, pH, DO, BOO, COO in Sukchon lake from May 1985 to Jan. 1986. ’rhe results were as follows. 1. Vertlcal descent of water temperature was the remarkable situation on Jul. and difference between surface layer and bottom layer was 3.50 C. 2. The value of pH was average 8.0 in both lake. The value of pH in the winter was average 7.6, and Summer was average 8.3. 3. ’rhe contents of dissolved oxygen was the hi뱅est value (1 3.0rngj~) in surface layer on Jan. and the lowest v외ue (8.1rngjQ) in bottom layer on Ul. 4. ’rhe contents of P04-P, NHa-N~ NOa-N and T-N were 0.03-o.17mgjQ, O .l 2 -o .34mgj~ ,0.36.91rngjQ and 0.76-1.37mgjQ, resctively.