(재)대구디지털산업진흥원 소개

김유현(Yoo-Hyun Kim) 한국콘텐츠학회 2008 한국콘텐츠학회지 Vol.6 No.2

취학 전 어린이들의 요충 감염 실태

김유현 ( Yoo Hyun Kim ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.3

The prevalence rate of Enterobius vermicularis eggs was investigated among preschool children located in the area of Iksan, Jeonbuk, during the period from March to July, 2005. Of the 1,082 children examined 78(7.2%) were found to be positive for E. vermicularis egg by the adhesive cellotape anal swab method. The egg positive rate of male and female were 6.7% and 7.8%, respectively. The positive rates range from 5.1% to 8.7% among preschool children, and the highest egg positive rate was observed in the 7 year old age group. Through this survey, it was determined that the E. vermicularis infection is still prevalent among children in Jeonbuk, Korea.

관절염 치료에 사용되는 한약재들의 항 염증 활성과 기전에 관한 연구

김유현 ( Yoo-hyun Kim ),박호 ( Ho Park ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.3

대식세포에서 염증반응이 진행되면, interleukin-6 (IL-6),tumor necrosis factor-. (TNF-.) 등의 cytokine들이 발현되어inducible nitric oxide synthase (iNOS), prostaglandin E2(PGE2) 등의 염증유발인자가 생성된다. 오가피, 우슬, 두충 각각의 추출물이 어느 정도의 항 염증 효능을 보이며 어떤 pro-inflammatorycytokine의 발현을 억제하는지에 대한 연구를 진행하였다. 오가피, 우슬, 두충은 물 추출하고 동결 건조시켰다. 각각의 추출물의 구성 성분들이 잘 추출되었는지 확인하기 위하여 지표물질인 acanthoside D, 20-hydroxyecdysone, pinoresinol diglucoside를 HPLC로 분석하였다. 항 염증 효능을 확인하기 위하여 lipopolysaccharide (LPS)로 RAW 264.7 세포주를 자극하여 염증반응을 일으킨 상태에서 각각의 추출물을 농도 별로 처리하고 NOassay를 통해 항 염증 효능을 확인하였으며 real time PCR로 pro-inflammatory cytokine들의 발현량을 측정하였다. 결과적으로 각각의 추출물은 지표성분들이 검출되었으며 오가피와 우슬이 두충보다 NO assay에서 높은 활성을 보였다. Cytokine 발현량측정에서는 오가피와 우슬은 iNOS와 IL-6의 발현을 억제하였고, 우슬은 TNF-.의 발현을 억제하였다. 우리나라는 전통적으로 약재를 조합하여 처방하여 왔다. 본 연구는 관절염에 전통적으로 사용해 오던 약재들이 어떤 기전에 의하여 항 염증 반응을 보이는지확인하고 이들을 조합하여 사용하였을 때 어떤 근거에 의하여 시너지 효능을 보이는지 확인하였다. Acknowledgements: 이 논문은 2016년도 원광보건대학교 교내연구비 지원으로 이루어졌으며 지원에 감사드립니다. When inflammatory reaction is in progress, the macrophages release inflammatory cytokines, such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and product inflammatory mediators, including inducible nitric oxide synthase (iNOS) and prostaglandin E2 (PGE2). We conducted this study to evaluate the anti-inflammatory efficacy on each water extract of Acanthopanacis cortex, Achyranthes radix, and Eucommiae cortex, and to investigate whether they inhibit the expression of pro-inflammatory cytokine. Acanthopanacis cortex, Achyranthes radix, and Eucommiae cortex were extracted with water and freeze-dried. Acanthoside D, 20-hydroxyecdysone, and pinoresinol diglucoside as an index material were analyzed by high-performance liquid chromatography (HPLC) to ensure that the components of each extracts were extracted well. RAW 264.7 cell line, stimulated with lipopolysaccharide (LPS) to cause an inflammatory response, was treated with each water extract at various concentrations to determine the anti-inflammatory efficacy. Then, the anti-inflammatory efficacy was confirmed by a nitric oxide (NO) assay, and the mRNA expression levels of pro-inflammatory cytokines were measured by real time PCR. As a result, the indicator materials were detected from each extract, and Acanthopanacis cortex water extract (ACWE) and Achyranthes radix water extract (ARWE) were shown to have a high activity than Eucommiae cortex water extract (ECWE) in NO assay. In Korea, traditionally it prescribed a combination of medicinal herbs. This study confirmed the anti-inflammatory response of these medicinal plants in arthritis and its synergistic effect when used in combination with western medicine.

내가 꿈꾸는 교회

김유현(Yoo Hyun Kim) 한국여신학자협의회 2013 한국여성신학 Vol.- No.77

Trichrome 염색에 의한 일부 아동들의 장내 기생충 감염상

김유현(Yoo-Hyun Kim) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.2

익산시내 거주하는 일부 아동들의 장내 기생충 감염 상태를 조사 하기 위하여 4세부터 8세사이의 유치원생과 초등학교 1~2학년 학생 510명(남 276명, 여 234명)을 대상으로 formalin-ether 침전법과 trichrome 염색법으로 대변검사를 실시하였다. 장내 기생 윤충란 및 원충류와 포낭 양성자는 62명(12.2%)이었으며, 윤충란 양성자는 3명(0.6%), 원충 포낭 양성자는 59명(11.6%)이었다. 성별 양성율은 남자(11.2%)보다 여자(13.2%)의 양성율이 다소 높았으며, 년령별 양성율은 7세군이 17.5%로 가장 높았고 4세군은 9.5%로 양성율이 가장 낮았다. 3종의 윤충란과 6종의 원충 포낭을 검출 하였으며, 윤충란은 요꼬가와흡충란 0.4%, 요충란 0.2% 및 편충란 0.2%였고, 원충의 포낭은 왜소아메바 4.1%, 대장아메바 3.9%, 람블편충 2.0%, 이질아메바 1.4%, 옥도아메바 0.8% 및 메닐편모충 0.4%였다. 윤충란 양성자 3명 중 단일종 감염이 2명(66.7%), 2종 감염은 1명(33.3%)이었으며, 원충류 포낭 양성자 59명 중 단일종 감염이 54명(91.5%), 2종 감염은 5명(8.5%)이었다. 연령별 원충 포낭의 양성율은 7세군이 14.3%로 가장 높았으며 4세 군은 9.5%로 가장 낮았다. This study was performed to observe the present status of intestinal parasitic infections among the children in Iksan, Jeonbuk, Korea. During the period from February to June 1996, total 510 fecal samples(male 276, female 234) were collected and examined by formalin-ether sedimentation technique and trichrome stain for intestinal helminths and protozoan cysts. Of 510 samples examined, 62(12.2%) were positive for intestinal parasites. Helminth egg positives were 3(0.6%), and protozoan cyst positives were 59(11.6%). The female group showed higher positive rate(13.2%) than males(11.2%), and the highest positive rates were observed in 7 age group(17.5%), and lowest positive rates were 4 age group(9.5%). 3 species of helminths egg and 6 species of protozoan cyst were detected; Metagonimus yokogawai 0.4%, Enterobius vermicularis 0.2%, Trichuris trichiura 0.2%, Endolimax nana 4.1%, Entamoeba coli 3.9%, Giardia lamblia 2.0%, Entamoeba histolytica 1.4%, Iodamoeba butschilii 0.8%, and Chilomastix mesnili 0.4%, respectively. In 3 helminth egg positive cases, single infection was 2(66.7%), and double infection 1(33.3%), among 59 protozoan cyst positives, single infection was 54(91.5%), and double infection was 5(8.5%). The highest positive rate of protozoan cyst was 7 age group(14.3), and lowest positive rate was 4 age group(9.5%).

건강 검진자 중 B형 간염 바이러스의 표면항원과 항체의 양성률

김유현 ( Yoo-hyun Kim ),신용섭 ( Yong-sub Shin ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

Hepatitis B virus(HBV) infection is prevalent in all parts of the world, with the highest incidence in Southeast Asia and Africa. Acute HBV infection is fully resolved in 90% to 95% of infected adults, who remain immune for their lifetimes. It is estimated that more than 350 million people in the world are chronic carriers. HBV carriers are at risk for developing chronic liver disease, including hepatocellular carcinoma. The aim of this study was performed to survey of the current tendency of HBV infection in Korea. A total number of 1,818 serum samples(male 1,292, female 526) collected from the health promotion center in Wonkwang University, School of Medicine and Hospital had been tested to detect for hepatitis B surface antigen(HBsAg) and anti-HBs by enzyme-linked immunosorbent assay(ELISA) method during the period March, 2002 to May, 2003. The positive rate for HBsAg was 4.5%(82/1,818), and that of male was 5.0% higher than female 3.2%. The positive rate of HBsAg by age group was the highest positive rate group in 40-49 years(6.1%), and upper 60 years(2.2%) was lowest of all. The positive rate for anti-HBs was 66.7%(1,212/1,818), and positive rate of female(66.9%) and male(66.6%) showed similar results. The positive rate of anti-HBs by age group was the highest positive rate group in 30-39 years(69.3%), and upper 60 years(59.8%) was lowest rate of all.

전북 지역의 Cryptosporidium oocyst 분포에 대한 역학적 조사

김유현 ( Yoo Hyun Kim ) 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1

전리 방사선의 조사가 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

김유현 ( Yoo Hyun Kim ),이규식 ( Gyu Sik Lee ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Nitric oxide (NO) have been suggested to plays an important role in immunologic defense and influences the functioning of secretory tissues and cells. It also exhbits cytotoxicf cytostatic activity as one of major effectors of the cellular immunity system. In this study, we investigated the effects of ionized radiation on the NO production in embryonic mouse liver cell line, BNL CL.2 cells. High-dose(6 Gy) of radiation induced production of NO in a dose-dependent manner from BNL CL.2 cells treated with IFN- r. Sma11 amounts of lipopolysaccharide (LPS) synergized with IFN- r in the production of NO from irra이ated same cells. Immunoblots showed induction of inducible nitric oxide synthase (iNOS). NO production in irradiated BNL CL.2 cells by IFN- r or IFN- r plus LPS was not affected by treatment with superoxide dismutase (SOD), but apparent1y decreased by the treatment with cata1ase (CAT) or SOD plus CAT. π1엉eres띠ts sugg않t that radiationinduced production of NO in BNL CL.2 cells might be attributed to H202 fonned by ionizing radiation.

B형 간염 바이러스의 표면항원과 항체의 양성율 조사

김유현 ( Yoo Hyun Kim ),김교순 ( Kyo Soon Kim ),김명신 ( Myoung Shin Kim ),서정미 ( Jung Mi Seo ),양경희 ( Yang Kyoung Hee ),정미라 ( Mi Ra Chung ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

Worldwide viral hepatitis is still recognized as a major public health problem particularly in developing countries. The most common etiologies are acute viral hepatitis A and B, and approximately half of the world’s population lives in regions of high hepatitis B virus(HBV) endemicity. HBV infection is hyperendemic in Korea. A Total number of 4,751 serum(ma1e 2,945, female 1,806) collected from the outpatients in Dae Han General Hospital had been tested to detect for hepatitis B surface antigen(HBsAg) and anti-HBs by reverse passive hemagglutination (RPHA) and passive hemagglutination (PHA) method during the period August, 2000 to July, 2001. The positive rate for HBsAg was 4.86%(231/4,751), and that of male was 5.20% higher than female 4.32%. The positive rate of HBsAg by age group was the highest positive rate group in 30-39 years(6.34%), and under 9 years(0.97%) was lowest of alL πle positive rate for anti-HBs was 37.82%(1,797/4,751), and positive rate of female was 41.42% higher than male 35.62%. The positive rate of anti-HBs by age group was the highest positive rate group in 10-19 years(4 1.37%), and under 9 years(20.39%) was lowest rate of all.

초등학교 어린이들의 장내 기생 윤충류 감염 실태 조사

김유현 ( Yoo Hyun Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.2

Intestinal parasitic infections remain a serious public health problem globally and have usually been associated with human malnutrition. This study was performed to observe the present status of intestinal helminthes infections among the primary school children in Iksan, Korea, during the period from June to August, 2006. A total of 974 fecal samples (male 479, female 495) were collected and examined by formalin-ether sedimentation technique for intestinal helminthes eggs. Of the 974 samples examined, 2 (0.2%) were egg positive for intestinal helminthes, and only eggs of Clonorchis sinensis were observed in the 2 cases. These C. sinensis egg were found in grade 5 (male, 1 case) and grade 6 (female, 1 case), respectively. The egg positive rate of male and female were 0.2%, respectively. Through this survey, I found that the C. sinensis infection is prevalent among primary school children in Iksan, Jeonbuk, and a continuous health education for school children is recommended to prevent the potential infection of C. sinensis.