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glucose Oxidase 효소전극 제조 화학적 방법에 의한 효소의 고정화
전재현,권형주,한미영,김두식 ( Jae Hyeon Juhn,Hyoung Joo Kwon,Mi Young Han,Doo Sik Kim ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1
Glucose oxidase was purified from Penicillium verruculosum, and immobilized by various chemical methods such as crosslinking of the enzyme with BSA by glutaral dehyde, covalent coupling with ω-aminohexyl agarose via glutaraldehyde as a bifunctional spacer group or covalent attachment of ω-aminohexyl agarose and the enzyme by amide bond. Construction of the enzyme electrode was carried out using the immobilized glucose oxidase, and the catalytic parameters as well as functional stability of the immobilized enzyme were monitored by oxygen electrode. Optimal pH of the glucose oxidase activity was shifted toward neutral side with more extended pH range as a result of immobilization and thermal stability of the catalytic function was also greatly improved compared to that of soluble enzyme. Practical application of the enzyme electrode for the determination of glucose concentration in biological fluids is demonstrated.
glucose Oxidase 효소전극 제조 : 화학적 방법에 의한 효소의 고정화 Immobilization of enzyme by chemical methods
김두식,한미영,전재현,권형주 생화학분자생물학회 1993 BMB Reports Vol.17 No.3
Glucose oxidase was purified from Penicillium verruculosum, and immobilized by various chemical methods such as crosslinking of the enzyme with BSA by glutaral dehyde, covalent coupling with ω-aminohexyl agarose via glutaraldehyde as a bifunctional spacer group or covalent attachment of ω-aminohexyl agarose and the enzyme by amide bond. Construction of the enzyme electrode was carried out using the immobilized glucose oxidase, and the catalytic parameters as well as functional stability of the immobilized enzyme were monitored by oxygen electrode. Optimal pH of the glucose oxidase activity was shifted toward neutral side with more extended pH range as a result of immobilization and thermal stability of the catalytic function was also greatly improved compared to that of soluble enzyme. Practical application of the enzyme electrode for the determination of glucose concentration in biological fluids is demonstrated.