http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김송학,Susanne U. Mertens-Talcott,Bipin Vaidya,Vinicius Paula Venancio,조세영,송종암,Boon P. Chew,권조셉,김두운 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.12
Quantitative reverse transcription PCR (qRTPCR)is a sensitive method for the detection of foodborneviruses in fecal samples. However, the performance ofqRT-PCR depends on the efficiency of virus concentrationmethods. In this study, the effect of Concanavalin A (ConA)-immobilized on polyacrylate beads (Con A-PAB) onthe qRT-PCR performance, in terms of sensitivity andspecificity to detect foodborne viruses in human fecalspecimens was compared with commercial viral RNAextraction kit (VRNA). The detection of foodborne virusesby qRT-PCR was validated by viral genome sequencing. Both Con A-PAB and VRNA methods were equally sensitiveand specific for detecting hepatitis A virus in fecalspecimens. Even though both methods showed highspecificity (100% vs. 100%) for detecting human norovirus(HuNoV), Con A-PAB method exhibited higher sensitivity(100% vs. 42.9%) and accuracy (100% vs. 73.3%) comparedto VRNA method. In conclusion, the application ofCon A-PAB would improve the performance of qRT-PCRfor the detection of HuNoV in fecal samples.