관절염 모델에서 가미청열사습탕(加味淸熱瀉濕湯)의 진통 및 소염 효과에 관한 연구

김일현 ( Il Hyun Kim ),이하일 ( Ha Il Lee ),이세원 ( Se Won Lee ),권영미 ( Young Mi Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2015 한방재활의학과학회지 Vol.25 No.1

Objectives This study was carried out to find the effects of Gami-cheongyulsaseub-tang (hereinafter referred to GCST) on the inhibition of zymosan-induced pain in rats and collagen II-induced arthritis (CIA) in DBA/1J mouse. Methods As an acute inflammatory pain model, peripheral inflammation was induced by intraplantar injection of zymosan into the right hind paw in rats and then the hyperalgesia and pain regulating factors in spinal cord were analyzed. As a chronic inflammation model, the mixture of collagen II and complete Freund``s adjuvant was treated into mice to establish rheumatoid arthritis and then body weight, thickness of hind paw, pathological change of spleen, immunological rheumatoid factor (IgG1, IgG2a, IgG2b, IgM and anti-collagen II), pro-inflammatory cytokines, and bone injury were analyzed. Results In the acute inflammatory pain model, GCST significantly inhibited the thermal and mechanical hyperalgesia and the pain regulating factors, including Fos, CD11b, PKA and PKC, in the spinal cord with a dose-dependent manner. In the chronic rheumatoid arthritis model, GCST administration decreased arthritic index and paw edema as compared with CIA control group. In particular, GCST reduced significantly the serum levels of total IgG2a, IgG2b, IgM, and specific anti-collagen II, but not total IgG1. GCST also resulted in the attenuation of bone injury and spleen enlargement/adhesion in CIA mice. Moreover, the secretion of pro-inflammatory cytokines TNF-α and IL-1β in CIA mice was significantly reduced by GCST in a dose-dependent manner. Conclusions Comparison of the results in this study showed that GCST had anti-nociceptive and immunomodulatory effects. These data imply that GCST can be used as an effective drug for not only rheumatoid arthritic pain but also other auto-immune diseases. (J Korean Med Rehab 2015;25(1):27-44)

해독금화산(解毒金花散)의 Methicillin-Resistant Staphylococcus aureus에 대한 항균효과

이하일 ( Ha Il Lee ),이수경 ( Su Kyung Lee ),권영미 ( Young Mi Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2015 한방재활의학과학회지 Vol.25 No.2

Objectives Methicillin-Resistant Staphylococcus aureus (MRSA) is a human pathogen and a major cause of hospital-acquired infections. New antibacterial agents that have not been compromised by bacterial resistance are needed to treat MRSA-related infections. In this study, we investigated the antimicrobial activity ofethanol extract of Haedokgeumhwa-san (HGH) which prescription is composed of korean medicine against MRSA. Methods The antibacterial activity of HGH extract was evaluated against MRSA strains by using the Disc diffusion method, broth microdilution method (minimal inhibitory concentration; MIC), checkerboard dilution test, and time-kill test; its mechanism of action was investigated by bacteriolysis, detergent or ATPase inhibitors. The checkerboard dilution test was used to examined synergistic effect of ampicillin, oxacillin, ciprofloxacin, vancomycin, gentamicin and norfloxacin in combination with HGH ethanol extract. A time-kill assay was performed a survival curve which was obtained by plotting viable colony counts depending on time on bacterial growth. Results The minimum inhibitory concentration (MIC) of ethanol extract (HGH) ranged from 1,000 to 2,000 μg/mL against all the tested bacterial strains, respectively. We are able to confirm that HGH extract has potentially strong antibacterial activity. In the checkerboard dilution test, fractional inhibitory concentration index of HGH in combination with antibiotics indicated synergy or partial synergism against S. aureus. A time-kill study showed that the growth of the tested bacteria was considerably inhibited after 8 hr of treatment with the combination of HGH with selected antibiotics. For measurement of cell membrane permeability, HGH 250~1,000 μg/mL along with concentration of Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (Tris) were used. In the other hand, N,N-dicyclohexylcarbodimide (DCCD) and Sodium azide (NaN3) was used as an inhibitor of ATPase. TX, Tris, DCCD and NaN3 cooperation against S. aureus showed synergistic action. Accordingly, antimicrobial activity of HGH was affected by cell membrane and inhibitor of ATPase. Conclusions These results suggest that Haedokgeumhwa-san extract has antibacterial activity, and that HGH extract offers a potential as a natural antibiotic against MRSA. (J Korean Med Rehab 2015;25(2):1-13)

가미한다열소탕(加味寒多熱少湯)이 고지방식이 비만생쥐의 체지방감소에 미치는 영향

이하일 ( Ha Il Lee ),이종하 ( Jong Ha Lee ),권영미 ( Young Mi Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2016 한방재활의학과학회지 Vol.26 No.1

Objectives In this study, it was investigated whether Gami-Handayeolso-Tang (HDYST) medication has anti-obesity effects in high fat diet (HFD)-fed obese mice. Methods The experimental animals were divided into five groups-normal diet-fed (ND), high fat diet-fed control (HFD), HFD+HDYST 150, HFD+HDYST 300, and HFD+orlistat as a positive drug. The obese markers such as body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, lipid contents, leptin, adiponectin, and GOT/GPT were measured. Also, white adipose tissue, liver weight, abdominal fat mass, hepatic lipid contents, and mRNA expression of obese-associating genes were examined in obese mice. Results In high fat diet-fed mice, HDYST administration significantly decreased body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, LDL-cholesterol, as well as leptin and GOT/GPT, compared to the HFD group in a dose-dependent manner. HDYST increased significantly the serum levels of HDL-cholesterol and adiponectin. It also reduced the accumulation of lipids, such as total lipid and triglycerides, in organs such as liver and abdominal adipose tissue. Moreover, HDYST administration significantly decreased the expression levels of fatty acid synthetic genes, such as sterol regulatory element- binding protein-1c (SREBP-1c), FAS and Stearoyl-Coenzyme A desaturase 1 (SCD-1), in the liver tissues, while it increased the messenger RAN (mRNA) levels of fatty acid catalytic genes, such as Peroxisome proliferator activated receptor alpha (PPAR-α), acyl-COA oxidase (ACO), and Carnitine palmitoyltransferase-1a (CPT-1a). Conclusions Based on the results above, HDYST reveals anti-obesity effects declining body fat accumulation through the regulation of fatty acid metabolism and leptin/ adiponectin serum levels. It therefore suggests that HDYST can be clinically useful for the treatment of obesity. (J Korean Med Rehab 2016;26(1):13-31)

가미청폐사간탕(加味淸肺瀉肝湯)이 고지방식이 비만생쥐의 체지방에 미치는 영향

금선오 ( Seon Oh Keum ),이하일 ( Ha Il Lee ),이종하 ( Jong Ha Lee ),윤용일 ( Yong Il Yoon ),권영미 ( Young Mi Kwon ),송용선 ( Yung Sun Song ) 한방비만학회 2015 한방비만학회지 Vol.15 No.2

Objectives: This study was designed to investigate the effect of Gami-cheongpyesagan-tang extract (GCST) on high fat diet-induced obesity in rats. Methods: The mice were divided into six groups; normal diet control, high fat diet control (HFD), HFD+GCST administrated group (100, 200, and 400 mg/kg) and olistat-admistrated group. Obesity was induced by high fat diet (45%) for 7 weeks in mice, and GCST was administrated orally every day for 7 weeks. The body weight, food intake, and serological markers such as total cholesterol, triglyceride, lipid contents, leptin, adiponectin and glutamic oxaloacetic transaminase/glutamic pyruvic transaminase were measured in mice. The mRNA expression of obese-associating genes such as sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase (FAS), stearoyl-CaP desaturase (SCD-1), peroxisome proliferator-activated receptor (PPAR)-α, COA oxidase (ACO), and carnitine palmitoyltransferase (CPT-1α) was analyzed by reverse transcription polymerase chain reaction. Results: The administration of GCST at 400 mg/kg, significantly reduced the increase of bodyweight and food intake as well as food efficiency compared to HFD group. GCST decreased the serum levels of triglyceride, total cholesterol, low-density lipoprotein-cholesterol, leptin in HFD control group and inhibited lipid accumulation in liver and adipose tissues, but did not increase high-density lipoprotein-cholesterol. In the liver tissues of GCST administrated HFD group, the mRNA levels of SREBP-1c, FAS and SCD-1 were decreased and the mRNA levels of PPAR-α, ACO, and CPT-1α were increased. Conclusions: These results indicate that GCST could improve high fat diet induced obesity through inhibiting the hyperlipidemia in fatty Liver. It suggest that GCST may be used clinically for declining the accumultion of body fat with hyperlipidemia.

육미지황탕가갈근(六味地黃湯加葛根)이 난소적출(卵巢摘出)로 유발(誘發)된흰쥐의 골다공증(骨多孔症)에 미치는 영향

이정우 ( Myung Kum Han ),권영달 ( Jae Kyun Won ),송용선 ( Chong Woo Lee ),원재균 ( Cheol Han Kim ),김철한 ( Young Dal Kwon ),권영미 ( Yung Sun Song ),권영미 ( Young Mi Kwon ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.4

Objectives :This study was performed to investigate the effects of Yukmijiwhang-tang (Liuweidihuang-tang) with Puerariae raidx(YMG) on the ovariectomized rat model of postmenopausal osteoporosis. Methods : We used 9 week aged Sprague-Dawley rat and divided into four group, sham operation group(Control, C), ovariectomized group(Negative Control, N.C), administrated YMG 1,250 mg/kg/day after ovariectomy group(T1), administrated YMG 2,500 mg/kg/day after ovariectomy group(T2). Then we observed changes in the body weight, body fat, serum metabolic products, bone density and so on. Results : 1. Body weight in negative control group showed significant increase in comparison with control group, but that in T1 and T2 group showed decrease in comparison with negative control group. 2. In the autopsy, negative control group showed uterus atrophy in comparison with control group. While T1 and T2 group showed decrease in uterus atrophy in comparison with negative control group. 3. In the total cholesterol, negative control group showed a significant increase in comparison with control group. While T1 and T2 group showed a significant decrease in comparison with negative control group. 4. In the body fat, negative control group showed a tendency to increase in comparison with control group. While T1 and T2 group showed a tendency to decrease in comparison with negative control group. 5. In bone density, normal control group showed a tendency to decrease bone density in comparison with control group. While T1 and T2 group showed a tendency to increase bone density in comparison with negative control group. Conclusions : YMG has shown to be capable of preventing and curing osteoporosis induced by ovariectomy osteoporosis rats

교통사고 환자 35예에 대한 한방의료 서비스 만족도 조사

박단서 ( Darn Seo Park ),필감흔 ( Chien Hsin Pi ),이정한 ( Jeong Han Lee ),공재철 ( Jae Cheol Kon ),백동기 ( Dong Gi Baek ),송용선 ( Yung Sun Song ),권영미 ( Young Mi Kwon ) 한방재활의학과학회 2008 한방재활의학과학회지 Vol.18 No.3

Objectives : This study aimed to understand the satisfaction degree of patients due to traffic accident(TA) in order to provide basic data which would give help not only to improve the quality of services, but also to strengthen the competitiveness of oriental medical hospitals on sequela of TA patients. Methods : The data were collected from inpatients and outpatients who answered a self-report questionnaire. Independent t-test, analysis of variance(ANOVA), X2-test, correlation analysis were used for data analysis. Results : According to the research on medical satisfaction degree, the overall satisfaction degree was 4.141. On each field, satisfaction degree related to the doctor service recorded the highest degree among others(4.474). Satisfaction degree related to the appropriateness of service was the second(4.091). On the other hand, satisfaction degree of using procedure was the lowest degree(3.857). On the difference of degrees of satisfaction by general characteristics, patients who were unemployed·houseworker group showed lower doctor service satisfaction degree than that of other groups. However, on the difference of degrees of satisfaction related to other general characteristics, there were no signigicant differences(p<0.05). Conclusions : The service distribution of oriental medical hospital was almost equal without concentrating on specified social classes.