http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
고태혁(T . H . Ko),이상호(S . H . Lee) 한국축산학회 1993 한국축산학회지 Vol.35 No.5
This study wars conducted to estahlish morphological anti biochemical changes occurring in the reproductive organs, testis, epididymis and their fluidal components in horse. Testis and epididymis were removed Irony Thoroughbred male aged 5∼6 year, and semen was collected with artificial vagina. The paraffin method and gel electrophoresis were used for the morphological and biochemical analysis, respectively. The size of testis was 29∼30 ㎝ in long axis and 26.5∼27.5 ㎝ in short axis and the weight was 210∼230 g which were in the range of normal measurements. The testis tised for this study demonstrated active spermatogenic activity in semiaiferous tubules showing various germ cells and other somatic components. Leyding, myoid, and Sertoli`s cells. The number of fractions in the caput and the corpus increased in comparison with that in the cauda epididymis. In particular changes in glycoproteins revealed by PAS staining suggest that glyeoproteins are rich in the cauda of epididymis, providing sufficient molecutes required for sperm maturation. Finally the similar glycoproteins were also found in the seminal plasma, indieating that they were originated form cauda region of epididymis. The results demonstrated that each luminal environments of epididymis that each luminal environments of epididymis possessed different protein components and that the glycoproteins might be closely related to the final stage of sperm maturation.
고태혁(T . H . Ko),이상호(S . H . Lee) 한국축산학회 1993 한국축산학회지 Vol.35 No.3
Present study was designed to establish a biological assay for the evaluation of the fertilizing capacity of equine spermatozoa. Equine spermatozoa were washed in mTAPL and preincubated for 4 h at an atmosphere of 5% CO₂ in air at 37℃. In homologous interactions, hamster spermatozoa showed 97.4% of penetration into the oocyte with fully decondensed 1∼8 sperm heads per oocyte. Seventy two percent of activation was obtained in heterologous interaction containing equine spermatozoa and zona-free hamster oocytes. However no sign of sperm decondensation was found although activation of oocyte chromosomes was evident. In view of results so far achieved, hamster oocytes may be less penetrable to equine spermatozoa than other sources of spermatozoa. However, the fertilizing ability of equine spermatozoa could be measured by the oocyte activation using zona-free hamster oocyte.