말정자 수정능력 판정을 위한 생물학적 방법의 확립 = Establishment of a Biological Assay for the Fertilizing Ability of Equine Spermatozoa

다국어 초록 (Multilingual Abstract)

Present study was designed to establish a biological assay for the evaluation of the fertilizing capacity of equine spermatozoa. Equine spermatozoa were washed in mTAPL and preincubated for 4 h at an atmosphere of 5% CO₂ in air at 37℃. In homologous interactions, hamster spermatozoa showed 97.4% of penetration into the oocyte with fully decondensed 1∼8 sperm heads per oocyte. Seventy two percent of activation was obtained in heterologous interaction containing equine spermatozoa and zona-free hamster oocytes. However no sign of sperm decondensation was found although activation of oocyte chromosomes was evident. In view of results so far achieved, hamster oocytes may be less penetrable to equine spermatozoa than other sources of spermatozoa. However, the fertilizing ability of equine spermatozoa could be measured by the oocyte activation using zona-free hamster oocyte.