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冠芽를 利用한 파인애플 '大農 5號'의 器內增殖에 關한 硏究
姜聖根,韓海龍 濟州大學校 亞熱帶農業硏究所 1988 亞熱帶農業硏究 Vol.5 No.-
파인애플 ‘大農 5號 ’의 冠芽를 器內培養하여 大量繁殖을 試圖할 境遇, NAA와 BA가 新梢生育에 미치는 影響과 摘合한 培地의 種類, 그리고 培養條件을 究明하고, 木圃 栽植時 培養苗와 吸芽苗의 生育을 比較하기 위하여 實施한 試驗結果를 要約하면 다음과 같다. 1. 新梢의 生育은 1/2 MS 培地에 蔗糖 4.0%, BA 1.0㎎/ℓ을 添加한 後 培養容器에 20%의 培地를 注入하여 액체진탕배양하는 것이 效果的이었다. 2. 不定芽를 繼代培養한 結果 chimera, albino 矮性形態의 變異個體가 5代째 6.1%, 6代째 15.9%가 發生되었다. 3. 뿌리의 發根에는 1/2 MS 培地에 活性炭 1.0g/ℓ을 添加한 後 1,500 ㏓의 光度條件에서 固體培養하는 것이 效果的이었다. 4. 揷木床에서의 發根은 器內培養時 100%에 比하여 IBA 2.0㎎/ℓ處理區에서 99.4%, NAA 2.0㎎/ℓ處理區에서 98.6%였으며, 그 다음이 無處理 89.7%, Auxibaron 56.6%, Rooton 54.7%, IBA 10.0㎎/ℓ50.5%, NAA 10.0㎎/ℓ46.2% 順이었다. 5. 器內培養苗는 吸芽苗보다 初期生育은 低調하였으나 時日이 經過함에 따라서 生育이 促進되었고, 栽植 後 360日째에는 草長 7.3㎝, 葉數 6.3個 程度가 增加되어 栽培期間 短縮이 期待되었다. The experiments were conducted to find out the effects of NAA and BA, to determine the optimal medium and the culture conditions for masspropagation of pineapple (Ananas comosus (L.)MERR) Daenong #5 (Smooth Cayenne ×Yellow Moritious), by using the crown explant, and to compare the degree of growth between plantlets obtained by shoot tip culture and the suckers. The results obtained were summarized as follows; 1. Multiple shoots were obtained by agitating half strength salts of Murashige and Skoog liquid medium containing 4.0% of sucrose and 1.0㎎/ℓof BA. 2. The varigated adventitious buds such as chimera, albino, and dwarf type were responsible for 6.1 and 15.9% at the 5th and the 6th subculture, respectively. 3. In vitro rooting was better in the half strength salts of MS solid medium containing 1.0g/ℓof the activated charcoal than in the liquid medium under the light condition of approximately 1,500 lux of light with a photoperiod of 16 hours. 4. In vitro rooting was easier than in vivo, and rooting percentages by each chemicals used were 99.4 in 2.0㎎/ℓIBA, 98.6 in 2.0㎎/ℓNAA, 56.6 in Auxibaron, 54.7 in Rooton, 50.5 in 10.0㎎/ℓIBA, 46.2 in 10.0㎎/ℓNAA and 89.7 in the control. 5. In general, the plantlets produced by in vitro culture showed poorer growth than the suckers at the early stage of growth after transplanted into soil mix, while in vitro cultured plantlets showed much better growth than the suckers at the late stage. The plantlets from in vitro culture were 7.3㎝ higher and produced 6.3 leaves more than did the suckers 360 days after transplanting.