한국의 요꼬가와 흡충 (吸蟲) ( Metagonimus yokogawai ) 에 관한 연구 - Ⅱ. 경상남북도산 (慶尙南北道産) 은어 ( Plecnglossus altivelis ) 의 지리적 분포 및 피낭유충의 (被囊幼蟲) 감염상태 -

송철용,전상린 ( Chul Yong Song,Sang Rin Jeon ) 한국하천호수학회 1983 생태와 환경 Vol.16 No.3·4

The present survey was undertaken to observe the geographical distribution of metacercaria among the Plecoglossus altivelis, the most important second intermeiate host of Metagonimus yokogawai, which were collected from 1979 to 1981 in Gyeongsang Bug Do and Gyeonsang Nam Do of Korea. The results obtained were summerized as follows: 1. A total of 120 fishes collected from the coastal areas of the above 18 rivers was examined for the metacercarial and 79(71.2%) among them were infected with metacercariae of the species, M. yokogawai. The number of metacercariae in a fishes ranged from 0 to 1,983 and the mean of the metacercariae in a fishes was 1,908. 9. 2. It was observed that the infection rates showed 46.6% in Gyeongsang Bug Do. The infection rates differed from river to river showing 100% in Yeongdeog Oshib River, Hyeongsan Riveer and Daejong River, 72.7% in Songcheon River, 60.0% in Nagdong River and 33.1% in Nanjeong River in order. And no metacercarial infections in their fish host were found in the areas of Pyeonghae Namdae River, Chugsan River and Buheung River. 3. And it was also obseverd that the infection rate were 95.7% in Gyeongsang Nam Do. The higest rates (100%) of metacercarial infection were observed in Taewha River, Jwacheon River, Jindong River, Hoihwa River, Gohyeon River, Gucheon River and the other areas such as Dundeog River, Hoiya River and Weolnae River showed 66.7%. From the results of the surveys, it turned out that metacercarial infection of P. altivelis with M. yokogawai were found in the coastal areas throughout Gyeongsang Nam do and excepting a few areas in Gyeongsang Bug Do.

肝吸蟲幼蟲과 成蟲의 運動性 및 趨化性에 關한 硏究 : I. 肝吸蟲 脫囊幼蟲의 몇몇 動物 膽液內에서의 生存能力 I. Viability of Excysted Metacercariae in the Bile of Several Animals

宋哲鏞,李純炯 중앙대학교 의과대학 의과학연구소 1980 中央醫大誌 Vol.5 No.1

The present study was undertaken to observe the viability of excysted metacercariae of Clonorchis sinensis in vitro when the larvae were incubated in the bile (B­bile) of cattle, dog, pig and fowl at 37℃. The survival rate and 50% lethal time of the incubated larvae were investigated. The results obtained were as follows: 1. The survival rates decreased according to the chronology of incubation in all kinds of bile juice. At first, rapid decline of the survival rates in various animal bile appeared during 4­12 hours after the incubation; i.e., 9 hours in cattle bile, 6 hours in the dog, 4 hours in the pig, and 12 hours in the fowl. On the other hand, the survival rate of control in saline decreased gradually during the incubation. 2. The maximum survival times of excysted metacercariae in various animal bile were 18 hours in cattle, 15 hours in dog and fowl, 12 hours in pig, whereas the larvae survived 48 hours in saline solution. 3. The 50% lethal times calculated by the formula of Reed­Muench(1938) were as follows; 11 hours 18 minutes in cattle bile; 11 h. 54 min. in fowl; 7 h. 51 min. in dog; 7 h. 48 min. in pig; and 20 h. 18 min. in the saline.

조류의 CO2배출량 측정

송철용 중앙대학교 문리과대학 1959 생록원 Vol.2 No.-

스파르가눔의 cysteine Proteinase 에 대한 단세포군 항체의 제조 및 특성 분석

송철용,김동수,최경희 ( Chul Y . Song,Tong S . Kim,Kyung H . Choi ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

A soluble thiol-dependent proteinase with molecular weight of 28 kDa was isolated and partially purified from Sparganum using ion exchange chromatography on DEAE-Triacryl M and Sepharose 4B affinity chromatography. This enzyme had maximal activity at pH 5.5 and showed inhibitor susceptibilities similar to the vertebrate acidic cysteine proteinases. One hybridoma cell clone, MASPC38 (Ig Gl) producing monoclonal antibody against partially purified cysteine proteinase was established. No cross reactivity was observed with six other parasite antigens. This antibody may be useful to detect the specific cysteine proteinase and/or circulating antigen of this parasite.

Spirometra Plerocerocoid ( sparganum ) 의 분비 배설물에서 Cysteine Proteinase 정제 및 부분 특성

송철용,최동호 ( Chul Yong Song,Dong Ho Choi ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

Adult worms of Spirometra plerocercoids (spargana) were dissected out from the tissues of naturally infected snakes (natrix trigria lateralia). Fresh spargana were incubated in distilled water and their excretory/secretory (ES) products were collected. Proteinse activity of the ES products was assayed with a synthetic fluorescent substrate, carbobenzoxy-phenylalanyl-arginyl-7-amino-4-trifluoromethylcoumarin. By means of DEAE-Trisacryl M ion-exchange chromatography and thiolpropyl-sepharose affinity chromatography, the proteinase in the ESP resulted in a 15.6-fold purification. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified materials revealed a single 28 kDa band, consistent with the apparent native molecular weight (gel filtration chromatography) of approximately 28 kDa. The proteinase purified from the ES products were examined for various biochemical characteristics : 1) its inhibitor profile indicated it belonged to cysteine endopeptidases; 2) it exhibited identical pH curves with optimal at pH 5.5 and a 50% activity range from pH 3.7∼7.1; 3) it completely degraded collagen chains to three identical products; and 4) it showed only minor activity toward hemoglobin. These results indicate that the proteinase may be involved in the parasite nutrition acquisition and/or penetration and lysis of host tissues.

한국의 요꼬가와흡충(Metagonimys yokogawai)에 관한 연구 IV. 동남부 지방산 은어의 지리적 분포 및 요꼬가와흡충 피낭유충의 감염상태

송철용,이순형,전상린,Song, Cheol-Yong,Lee, Sun-Hyeong,Jeon, Sang-Rin 대한기생충학회 1985 The Korean Journal of Parasitology Vol.23 No.1

The present survey was undertaken to observe the geographical distibution of matacercaria among the sweetfish, Plecoglossus altivelis, the most important second intermediate host of Metagonimus yokegawai, which were collected during the period from 1983 to 1984 in south-eastern coastal areas of Korea. The results obtained were summarized as follow: 1. A total of 668 sweetfish collected from 70 chins (=streams) in the above coastal arcus was examined for the metacercariae, and 432 (64.7%) among them were infected with metacercariae of M. yokogawai. The mean number of metacercariae in a fish was in the range from 0 to 29,604 and the mean number of the metacercariae in a fish was 640.3. 2. It was observed that the mean infection rate showed 46.45 in Gangweon-Do (=province) . The infection rates varied from stream to stress showing 100% in Namdae stream(Gangnun-city) , Obsib stream (Samchok-gun) and Dongmak, 95.0% in Hosan stream, 90.0% in Gungchin stream, and Namdae stream (Yangyang-fp) ,80.0% in Hwasang stream,50.0% in Daebong stream,45.0% in Yonghwa stream, 30.0% in sangchsn stream and Sachsn stream, 20.0% in Munam stream and Okgyoe stream, 15.0% in Imweon stream, 10.0% in Namchsn stream. And no metacercarial infections in their fish host were found in the areas of Mulchi stream, Sangun stream, Gosamun stream, Jonchon stream and Gagok stream. 3. It was observed that the mean infection rate showed 50.9% in Kyangsangbuk-Do (=province). The infection rates also varied from stream to stream showing 100% in Songchon stream, Osib stream (Yongdok-gun) and Daejong stream,60.0% in Pyanghae stream and Gunmu stream,50.0% in Buhwung stream and stream, 25.0% in Nagok stream, 20.0% in Oangbi stream, 10.0% in Namhodong stream. And no metacercarial infections in their fish host were found in the areas of Namdae stream (Uljinup) and Chaksan stream. 4. It was observed that the mean infection rate showed 67.6% in Kyangsangnam-Do(=province). The infection rates varied from stream to stream showing 100% in Taehwa river, Hwiya river, Inchfn stream, Miryang river, Nam river, Hwagae stream, Koha stream, Sosang stream, and Tongchon stream,90.9% in Yancho stream,90.0% in Hoihwa stream,80.0% in Chindong stream, 66. YoA in Chingyo stream, 40.0% in Hoyam river. And no metacercarial infections in their fish host were found in the areas of Chinjon stream, Sanchan stream and Jukch6n stream. 5. It was observed that the mean infection rate showed 94.6"A in 0h511anam-Do(=province). The infection rates varied from stream to stream showing 100% in DongchEn stream, Isachon stream, Somjin river(GoksEng-gun) , Ssmjin river(Kurye-gun) , S6si stream, Gokchsn stream, Kohung stream, Kwansan stream, Youi stream, stream, Unj6n stream and Apnae stream, 92.3% in Tamjin river, 90.9% in Okkok stream, 84.6% in Songgen stream. And no metacercarial infections in their fish host were found in the area of Ypmgpl stream. 6. On the other hand, the infection rate showed 100% in Inchsn river of Chollabuk Do (=province) (=province) and Kangsn stream of Cheju-Do(=province) . 이 보고는 우리나라 동남부지방(강원도, 경상남.북도, 전라남.북도, 제주도)에 위치하고 있는 70개 하천에서 1983년과 84년 6월 하순부터 10월 초순 중에 채집한 은어(Plecoglossus altivelis)의 요꼬가와흡충(Metagonimus yokogawai) 피낭유충의 감염상태를 조사한 결과이다. 1. 조사지역 70개 하천에서 채집한 은어의 총수는 668개체였으며 이들 가운데 432개체에서 요꼬가와흡충 피낭유충이 검출되어 48.7%의 감염률을 보였다. 2. 강원도의 20개 하천(남천, 문암천, 불치천, 쌍천, 양양군의 남대천, 전천, 삼척군의 오십천, 궁촌천, 동막천, 용화천, 임원천, 호산천, 가곡천)에서 채집한 237개체의 은어를 조사한 결과는 110개체에서 피낭유충이 검출되어 그 감영률은 46.4%이었다. 3. 경상북도의 12개 하천(나곡천, 울진읍의 남대천, 왕비천, 척산천, 평해천, 군무천, 송천, 남호동천, 부홍천, 영덕읍의 오십천, 지경천, 대종천)에서 채집한 112개체의 은어를 조사한 결과는 57개체에서 피낭유충이 검출되어 그 감염률은 50.9%에 이르렀다. 4. 경상남도의 17개 하천(태화강, 회야강, 효암천, 이촌천, 밀양강, 진전천, 진동천, 산촌천, 연초천, 회화천, 남강, 진교천, 화계천, 고하천, 죽천천, 서상천, 동천)에서 채집한 139개체의 은어를 조사한 결과는 94개체에서 피낭유충이 검출되어 그 감염률은67.6%이었다. 5. 전라남도의 19개 하천(동천, 옥곡천, 이사천, 곡성천, 곡성군의 섬진강, 연곡천, 구례군의 섬진강, 서시천, 곡천, 고흥천, 성근천, 관산천, 여의천, 수문천, 해창천, 탐진강, 수양천, 어송천, 운전천, 앞내천)에서 채집한 167개체의 은어를 조사한 결과는 158개체에서 피낭유충이 검출되어 그 감염률은 94.6%를 보였다. 6. 전라북도의 인천강에서 채집한 1개체의 은어를 조사한 바 피낭유충이 검출되었다. 7. 그리고 제주도의 강전천에서 채집한 12개체의 은어를 조사한 바 모든 개체에서 피낭유충이 검출되어 100%의 감염률을 보였다.

韓國의 요꼬가와 吸蟲(Metagonimus yokogawai)에 關한 硏究 2 : 慶尙南北道産 은어( Plecnglossus altivelis)의 地理的 分布 및 被囊幼蟲의 感染狀態

宋哲鏞,田祥麟 한국하천호수학회 1983 생태와 환경 Vol.16 No.3·4

Partial Characterization of Cysteine Proteinase Purified from Excretory Secretory Prouducts of Spirometra Plerocercoids

송철용,최동호,Song, Chul-Yong,Choi, Dong-Ho 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

Adult worms of Spirometra plerocercoids (spargana) were dissected out from the tissues of naturally infected snakes (natrix trigria lateralia). Fresh spargana were incubated in distilled water and their excretory/secretory (ES) products were collected. Proteinse activity of the ES products was assayed with a synthetic fluorescent substrate, carbobenzoxy-phenylalanyl-arginyl-7-amino-4-trifluoromethylcoumarin. By means of DEAE-Trisacryl M ion-exchange chromatography and thiolpropyl-sepharose affinity chromatography, the proteinase in the ESP resulted in a 15.6-fold purification. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified materials revealed a single 28 kDa band, consistent with the apparent native molecular weight (gel filtration chromatography) of approximately 28 kDa. The proteinase purified from the ES products were examined for various biochemical characteristics : 1) its inhibitor profile indicated it belonged to cysteine endopeptidases; 2) it exhibited identical pH curves with optimal at pH 5.5 and a 50% activity range from pH 3.7~7.1; 3) it completely degraded collagen chains to three identical products; and 4) it showed only minor activity toward hemoglobin. These results indicate that the proteinase may be involved in the parasite nutrition acquisition and/or penetration and lysis of host tissues. 한국산 뱀(유혈목이 : Natrix tigrina lateralis) 에서 스파르가눔(sparganum) 충체를 수집하고, 그들의 분비 배설물을 ion-exchange chromatography와 affinity chromatogrphy를 실시하여 cysteine proteinase를 순수 정제하였다. 정제된 효소의 최적 pH는 5.5이었고, 최적 mol 농도는 50 mM(50 mM sodium acetate, pH 5.5)이었다. 정제된 효소는 thiol-dependent이고, $4^{\circ}C$와 $37^{\circ}C$에서 24시간 동안 95%의 안전성올 보였다. 효소의 활성도는 저분자 합성기질인 CBZ-phe-arg-AFC에 대하여 활성이 높았다. 정제된 효소는 cysteine proteinase 특이 억제인자인 E-64, NEM, iodoacetic acid에 의해 활성이 완전히 억제되었고, serine proteinase 특히 억제인자, aspartic proteinase 특이 억제인자 그리고 metallo proteinase 억제인자에 의해 활성이 억제되지 않았다. Ultrogel AcA 54 column chromatography로 정제된 cysteine proteinase의 분자량을 측정한 결과 28 kDa이었다. 정제된 효소는 collagen type I과 hemoglobin을 분해하였다. 이상의 결과에서 스파르가눔은 cysteine proteinase를 이용하여 숙주 체내이동, 조직 침투성 및 영양소 섭취에 관여하는 것으로 생각된다.

肝 卵圓細胞 增殖에 關한 實驗的 硏究 : 特히 膽管纖維症, 膽管異形增殖 및 膽管細胞癌 間의 相關 Relationship Among Cholangiofibrosis, Cholangiodysplasia and Cholangiocarcinoma

宋哲用,金勇一,李濟九 서울의대 1975 서울의대잡지 Vol.16 No.1

韓國人에서 分離된 淋菌의 藥劑耐性에 關한 硏究

宋哲鏞,金汶普 중앙대학교 기술과학연구소 1984 기술과학연구소 논문집 Vol.14 No.-

The measurement of the sensitivity of gonococci to antibiotics is great importance in the rational uses of chemotheraphy, evolution of new agents and epidemiological studies. Especially, penicillinase-producing Neisseria gonorrhoeae (PPNG) must be regarded as a serious threat in public health. In connection with recent increasing discoveries of PPNG strains, this study was proposed to investigate the isolation rates of N. gonorrhoeae to the high risk populations and antimicrobial susceptibility of current strains. The strains of N. gonorrhoeae tested Seoul, Incheon, Kyungki, Gangwon, Chung Buk, Chung Nam, Kyung Buk and Busan were collected April to September in 1982. Antibiotics used for the susceptibility test by the agar dilution method (Wilkinson 1980) were penicillin-G, ampicillin, tetracycline, chlorampenicol, cefuroxime, spectinomycin, kanamycin, sulfamethoxazole and trimethoprim. The result was summarized as follows: The isolation rates of N. gonorrhoeae of the prostitute girls were about 7.2% and determinated PPNG strains were about 20.2%. However, according to the areas, there were many differences about the determination rate of PPNG from 0% to 41.7%. PPNG strains were shown higher resistant to penicillin-G and ampicillin than non-PPNG strains. The minimal inhibitory concentration (MIC) range of penicillin-G was 0.25 ㎍/ml∼64 ㎍/ml and 19 Cultures (20.21%) were resistant (MIC≥4.0 ㎍/ml). MIC range of ampicillin was 0.25 ㎍/ml ∼ 32 ㎍/ml and 76 cultures (80.8%) had MIC≥4.0 ㎍/ml. Spectinomycin was susceptible to all strains of PPNG and non-PPNG which were in MIC≤16 ㎍/ml. MIC range of sulfamethoxazole was 16 ㎍/ml ∼ 512 ㎍/ml and MIC range of sulfamethoxazole plus trimethoprim was 8 ㎍/ml ∼ 64 ㎍/ml. MIC range of cefuroxime was 0.125 ㎍/ml ∼ 0.5 ㎍/ml and cefuroxime was susceptible to all strains of PPNG and non-PPNG which were in MIC≤0.5 ㎍/ml.