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( Patchanee Prapas ),( Chomporn Chokboonmongkol ),( Karl Hans Zessin ),( Thomas Alter ),( Sarinya Pornaem ),( Nipa Chokesajjawatee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.11
We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n=16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
Prapas Patchanee,Pakpoom Tadee,Orapun Arjkumpa,David Love,Karoon Chanachai,Thomas Alter,Soawapak Hinjoy,Prasit Tharavichitkul 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.4
This study was conducted to determine the prevalence oflivestock-associated methicillin-resistant Staphylococcus aureus(LA-MRSA) in pigs, farm workers, and the environment innorthern Thailand, and to assess LA-MRSA isolatephenotypic characteristics. One hundred and four pig farmswere randomly selected from the 21,152 in Chiang Mai andLamphun provinces in 2012. Nasal and skin swab sampleswere collected from pigs and farm workers. Environmentalswabs (pig stable floor, faucet, and feeder) were also collected. MRSA was identified by conventional bacterial culturetechnique, with results confirmed by multiplex PCR and multilocus sequence typing (MLST). Herd prevalence of MRSA was9.61% (10 of 104 farms). Among pigs, workers, and farmenvironments, prevalence was 0.68% (two of 292 samples),2.53% (seven of 276 samples), and 1.28% (four of 312 samples),respectively. Thirteen MRSA isolates (seven from workers,four from environmental samples, and two from pigs) wereidentified as Staphylococcal chromosomal cassette mec IVsequences type 9. Antimicrobial sensitivity tests found 100% ofthe MRSA isolates resistant to clindamycin, oxytetracycline, andtetracycline, while 100% were susceptible to cloxacillin andvancomycin. All possessed a multidrug- resistant phenotype. This is the first evidence of an LA-MRSA interrelationship among pigs, workers, and the farm environment in Thailand.