최근 충북지역에서 분리된 Gram 양성 세균종과 항생물질의 감수성 양상

황석연,최원창 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

임상검체에서 분리되는 병원균을 주기적으로 분석하고 이에 대한 항균제 감수성의 변화 양상을 파악하는 것은 임상에서의 적절한 항균제의 선택이나 내성균의 감독과 조절을 위해 필수적이라 할 수 있다. 저자들은 충북대학병원에 1996년 1년간 내원 환자의 미생물 배양 검사에서 분리, 동정된 1,689 균주중 Gram양성인 542 균주의 분리 빈도 및 주요 균종에 대하여 항생제 감수성 양상을 조사하였다. Gram 양성 균종은 S. aureus, S. pneumoniae, S. epidermidis, S. hemolyticus, coagulase negative Staphylococcus (CNS), Enterococcus faecalis 순으로 분리되었다. 한편, 항생제 감수성 시험 결과 S. aureus의 항생제 감수성은 penicillin과 gentamicin에 대한 저항성이 현저히 높았으며, teicoplanin과 vancomycin(MlC50 0.5㎍/ml)에 감수성이 높은 것으로 나타났다. CNS의 경우도 S. aureus와 대동 소이한 결과를 보였으나, S. epidermidis의 경우에는 ciprofloxacin과 clindamycin에 감수성이 높은 것으로 나타났다. Enterococcus spp.는 vancomycin, penicillin, tetracycline 등에 저항성 이 매우 높았으며, 검사한 모든 항생제에 대하여 대부분의 감수성이 zig-zag pattern을 보였다. In order to control resistant strains and to properly select the antimicrobial agents, it is of quite importance to know current trends of bacterial species and changing patterns of antimicrobial resistance rates. The authors studied the results of 542 Gram-positive strains among 1,689 strains isolated at Chung-buk National University Hospital in 1996. The frequently isolated Gram-positive microorganisms were Staphylococcus aureus, Streptococcus, pneumoniae, Staphylococcus epidermidis and Enterococcus faecalis in descending order. S. aureus showed high resistance to penicillin, gentamicin, and susceptibility to teicoplanin and vancomycin. Coagulase negative Staphylococcus was highly resistant to all of the antibiotics used in this experiment except teicoplanin and vancomycin. Enterococcus were highly resistant to vancomycin, penicillin and tetracycline. MIC of Gram- positive oaganisms was appeared to be zig-zag pattern.

임상가검물과 파라핀 포매 조직에서 PCR법을 이용한 결핵균의 검출

김은중,최우순,황석연 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

PCR을 이용한 임상가검물 중에서 보편화된 객담 이외에 미량의 각종 체액과 임상에서 많이 실시하지 않는 파라핀 포매 조직에서의 결핵균 검출을 실험하여 그 활용 가능성을 규명하고자 하였다. 임상가검물인 체액 65예는 항산성 염색과 배양검사, PCR을 실시하였고, 파라핀 포매 조직 50예는 항산성 염색과 병리조직학적 진단, PCR을 실시하여 다음과 같은 결론을 얻었다. 본 실험 검체 중 임상가검물인 체액에서 항산성 염색 음성인 검체 중 12.1%,배양검사에서 음성인 검체 중 3.7%에서 PCR양성의 결과를 보였고, 파라핀 포매 조직에서는 항산성 염색 음성인 검체 중 20.0%에서 PCR양성의 결과를 얻어 PCR이 민감도와 특이도가 높음을 확인할 수 있었다. This study has been carried out to investigate the sensitivity of polymerase chain reaction (PCR) method over conventional acid-fast bacilli (AFB) staining and/culture methods for the detection of Mycobacterium tuberculosis from trace body fluid and paraffin-embedded tissues (PET) specimens. A total of 65 cases were employed for the AFB staining and culture test, and a total of 50 cases were subjected to PCR and histopathological analysis. Among the specimen showing negative reaction to AFB staining, 12.1% were positive to PCR and 3.7% of the specimen representing negative result to culture test showed positive reaction to PCR. In addition, 20.0% of the specimen with AFB negative showed positive reaction to PCR. From these results, it could be concluded that PCR method overwhelms AFB staining and culture tests in sensitivity and specificity to .M. specificity to tuberculosis detection.

랫드에서 미생탕의 4주 반복투여독성

김태명, 류재면, 서임권, 박동선, 조정희, 장용훈, 김윤배, 황석연 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

Effect of Korean Red Ginseng Crude Saponin on Blood Chemical Parameters of Guinea Pigs Exposed to TCDD

Hwang, Seock-Yeon,Youn, Nae-Young 대한의생명과학회 2003 Biomedical Science Letters Vol.9 No.1

This study was carried out to investigate the effect of crude ginseng saponin (CGS) on blood chemical parameters in adult female guinea pigs exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). A total of 80 guinea pigs (800±20g) were divided into 8 groups: group 1 (normal control group) was given vehicle (corn oil containing small amount of acetone and DMSO) and saline;group 2 (single TCDD-treated) received TCDD (1ug/kg, i.p.) and saline (i.p.);groups 3 and 4 were administered CGS at a daily i.p. doses of 10 and 20 mg/kg for 4 weeks, respectively; groups 5 and 6 were administered CGS (10 and 20mg/kg, respectively) for 5 weeks starting 1 week before TCDD-exposure; groups 7 and 8 were administered CGS(10 and 20 mg/kg, respectively)for 3 weeks from 1 week after TCDD-exposure, CGS was prepared by Diaion HP-20 adsorption chromatography. Body weights of G2 were significantly decreased from the 2nd week after TCDD-exposure(p<0.01). Body weights of the CGS-treated groups were also decreased by TCDD-exposure bot the weight loss was greatly retarded compared with that of G2. Increase in blood glucose, amylase, lipase, total cholesterol, triglyceride, AST and LDL-cholesterol levels by TCDD exposure was significantly attenuated by the CGS-treatment(p<0.05). From these results, we found that saponin, the main active ingredient of ginseng, played a protective role in TCDD-induced toxicity and ginseng protected female animals from dioxin-induced toxic manifestation.

Protective Effect of Panax ginseng against Dioxin-induced Testicular Toxicity in Guinea Pigs

Seock-Yeon Hwang,Jae-Joon Wee,Nae-Young Youn,Si-Kwan Kim 한국실험동물학회 2003 Laboratory Animal Research Vol.19 No.2

Preventive Effect of Protaetia brevitarsis Extract against Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Rats

Seock-Yeon Hwang,Yun-Bae Kim,Jong Suk Kim,Jae-Sam Hwang,Sang-Han Lee,Chi-Young Yun 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1

This study was carried out to investigate preventive effects of Protaetia brevitarsis extract (PBE) against the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced toxicity in 7-week-old Sprague-Dawley male rats. Forty rats were treated with PBE (50, 100 or 200 ㎎/㎏) for 2 weeks before intraperitoneal injection of TCDD (50 ㎍/㎏). TCDD induced hepatocelluar degeneration and necrosis leading to increases in serum aspartate amintransferase (AST) and alanine aminotransferase (ALT) levels. In biochemical analyses, thiobarbituric acid-reactive substances (TBARS), superoxide dismutase (SOD) and catalase in hepatic tissues were remarkably increased by TCDD treatment, suggestive of lipid peroxidation and the induction of antioxidant enzymes such as SOD and catalase. Not only increases in serum AST and ALT, but also induction of lipid peroxidation and antioxidant enzymes in hepatic tissues caused by TCDD were significantly attenuated by PBE treatment in a dose-dependent manner. Such hepatoprotective effects of PBE were confirmed by histopathological examinations, wherein only mild hepatocytic vacuolations were observed in the liver of rats treated with a high dose (200 ㎎/㎏) of PBE in comparison with severe hepatocytic degenerations administered with TCDD alone. From these results, it is suggested that PBE could be a promising candidate for the protection of liver injury, based on the preventive effects on the morphological and biochemical parameters of hepatocytic injury.

Effect of Yacon on Platelet Function in Hypercholesterolemic Rabbits

( Seock Yeon Hwang ),( Yong Lim ),( Dong Ju Son ),( Yun Bae Kim ),( Bang Yeon Hwang ),( Yeo Pyo Yun ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.4

Hypercholesterolemia indirectly increases the risk of arterial and venous thrombosis by enhancing the ability of platelets to aggregate. Yacon (Smallanthus sonchifolius) is composed of fructooligosaccharides, proteins, minerals and phenolic compounds, and has potential benefi ts for the management of diabetes. This study investigated whether the consumption of yacon in the diet inhibits platelet aggregation under hypercholesterolemic conditions. Male New Zealand white rabbits were fed one of fi ve dietary interventions: a normal control diet, 0.5% cholesterol diet, 0.5% cholesterol diet+a low dose of yacon (0.5 g/kg body weight given orally each day), 0.5% cholesterol diet+a high dose of yacon (2.5 g/kg body weight given orally each day), or a 0.5% cholesterol diet+lovastatin (2 mg/kg body weight given orally each day). After 8 weeks, blood was collected to measure the amount of collagen- and thrombin-induced platelets present. Yacon inhibited the platelet aggregation induced by low doses of agonists (0.5 μg/ml collagen and 0.02 units/ml thrombin) in a concentration-dependent manner. In addition, yacon concentration-dependently inhibited collagen-induced arachidonic acid liberation. Moreover, n-hexane, chloroform and ethyl acetate fractions showed a marked and selective inhibition of the platelet aggregation induced by collagen, again in a dose-dependent manner. These fractions, especially that of chloroform, signifi cantly suppressed platelet aggregation. The results of this study demonstrate that when yacon is added to a cholesterol-enriched diet, cholesterol-induced platelet aggregation returns to control levels. This may also be benefi cial in preventing atherosclerosis and reducing risk factors for coronary artery disease and stroke.

Protective Effect of Panax ginseng on TCDD-induced Liver Toxicity in Male Guinea Pigs

Hwang, Seock-Yeon,Wee, Jae-Joon,Kim, Si-Kwan,Youn, Nae-Young 대한의생명과학회 2003 Biomedical Science Letters Vol.9 No.1

This study was carried out to investigate the preventive and therapeutic effect of panax ginseng water extract (PG-WE) on the toxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), one of the most notorious toxic environmental pollutants belonging to the group of polyhalogenaed aromatic hydrocarbons. Normal control (NC) group guinea pigs (180∼200g)received vehicle and saline, and TCDD-treated (TT) group was given TCDD and saline. P100 and P200 group animals received PG-WE for 28 days since 1 week before TCDD exposure at daily doses of 100 mg/kg b.w. and/or 200 mg/kg b.w., resectively. C100 and C200 group received PG-WE for 14 days stating 1 week after TCDD-exposure. Toxicity was induced by a single intraperitoneal injection of TCDD (1 ug/kg b.w.). Abnormal increase in AST and ALT activities in TT group was significantly improved by the administration of PG-WE. Microscopically, there were mild to moderate swelling of hepatocytes, hyperchromatism of individual cells, acidophilic cytoplasm and cytoplasmic vacuolation of some hepatocytes, slight to moderate variations of staining density, occasional single cell necrosis, variable size and shape of some hepatocytes, small groups of degenerating hepatocytes surrounded by mononuclear cells, dilated sinusoids of centrilobular zone and some loss of lobular architecture in TT group liver. From these results, we could find the protective and therapeutic role of PG-WE in TCDD-induced liver toxicity by examining the blood chemical parameters and histopathological observation.

Dose-Dependent Toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Male Guinea Pigs

Seock-Yeon Hwang,Jin Bae Yang,Jae-Joon Wee,Jong-Soo Kyung,Yun-Bae Kim,Seung Bok Hong,Si-Kwan Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3

This study was carried out to determine the dose-dependent toxic response of male guinea pigs to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD). Male Hartley guinea pigs (200±20 g) received single intraperitoneal administration of 2,3,7,8-TCDD at doses of 0.1, 0.25, 0.5 or 1 ㎍/㎏. Body weight gain, organ weights, blood biochemistry and sperm quality (sperm head counts and motility pattern) were determined. Body weight, testicular weight, sperm head counts and ratio of sperm with straightforward progressing were significantly decreased by 2,3,7,8-TCDD in a dose-dependent manner. Blood biochemistry panels were worsened remarkably by 2,3,7,8-TCDD exposure. These results provide with information on the dose-responses of male guinea pigs to 2,3,7,8-TCDD, especially on the toxicity of testes, the most-sensitive target organ.

Panax ginseng Improves Senile Testicular Function in Rats

Hwang, Seock-Yeon,Sohn, Sang-Hyun,Wee, Jae-Joon,Yang, Jin-Bae,Kyung, Jong-Soo,Kwak, Yi-Seong,Kim, Sung-Won,Kim, Si-Kwan The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.4

We reported previously that the administration of Korean red ginseng water extract (KRG-WE) protected the guinea pig testis against damage induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (a potent endocrine disruptor). We also found that crude saponin from ginseng was the active ingredient responsible for this protection. Here, we examined the biological role of KRG-WE in an animal model of age-induced dysfunction of spermatogenesis. Twenty-four male Sprague-Dawley (six 2-month-old and eighteen 12-month-old) rats were used. The young and old control groups received only vehicle. The ginseng saponin (GS)- and KRG-WE-treated groups received GS (40 mg/kg body weight/day) and KRG-WE (200 mg/kg body weight/day), respectively, for 4 months. The number of cells, Sertoli cell index, Johnsen's score, and sex hormone levels decreased significantly with age. However, the administration of KRG-WE and GS markedly improved the number of germ cells, seminiferous tubular size, and Johnsen's score in the old rats. Ginseng produced a distinct testicular histological improvement in old rats. KRG-WE and GS elevated testosterone levels, while attenuating the aberrant increase in follicle stimulating hormone and luteinizing hormone levels. Sperm kinematics evaluated by a computer-assisted sperm analyzer demonstrated improvement in the percentage of motile sperm, progressive sperm motility, and curvilinear velocity associated with sperm quality, supporting the beneficial role of red ginseng in senile spermatogenesis. Overall, the total water extract had a more potent effect than the corresponding saponin fraction. In conclusion, Korean red ginseng rejuvenated age-induced testicular dysfunction. Additionally, the total water extract was more potent than the corresponding saponin fraction.