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( Michal Sposob ),( Tae-hoon Kim ),( Yeo-myeong Yun ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-
The biological oxidation of sulfide (HS<sup>-</sup>) to elemental sulfur (S<sup>0</sup>) seems to be one of attractive and sustainable ways for HS<sup>-</sup>removal from organic wastes. Sulfide oxidizing bacteria (SOB) are responsible for biological oxidation of HS<sup>-</sup> under NO<sub>3</sub> <sup>-</sup> presence as an electron acceptor. The S<sup>0</sup> obtained from biological waste treatment processes is characterized by better fertilizer properties than the S<sup>0</sup> from physicochemical recovery. The objective of the present study was to evaluate the impact of N/S ratio (range: 0.35-1.30) and temperature (range: 25-10℃) on the S<sup>0</sup> accumulation in expanded granular sludge bed (EGSB) reactor. During the 150 days of experiment NO<sub>3</sub> <sup>-</sup> was almost completely removed (except N/S=1.30). The highest HS<sup>-</sup> removal was obtained at 25℃ at N/S=0.35 (98.0%), while the lowest occurred at mid-N/S ratios (N/S=0.6) at 10℃ that was equal to 76.9%. The imposed N/S ratio and temperature changes in reactor had a substantial impact on HS<sup>-</sup> oxidation products. By decreasing a temperature and increasing the N/S ratio, the accumulated elemental sulfur (S<sup>0</sup> <sub>acc</sub>) decreased while the other sulfur components like suspended elemental sulfur (S<sup>0</sup> <sub>ss</sub>) and SO<sub>4</sub> <sup>2-</sup> increased.
혐기성소화 공정에서의 황 환원균의 선택적 배양을 위한 연구
김태훈 ( Tae-hoon Kim ),( Michal Sposob ),임병서 ( Byung-seo Lim ),윤여명 ( Yeo-myeong Yun ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2019 No.-
The anaerobic sulfidogenic reactors are a promising option for the sulfur presence reduction in feedstock prior the regular anaerobic digestion. In sulfidogenic operation the sulfate reducing bacteria (SRB) reduce sulfate (SO<sub>4</sub> <sup>2-</sup>) to H2S that is released to the gas phase. In this process the organic carbon as electron donor is required as for methanogenesis. The objective of this study was to enrich SRB presence and stop the methanogenic activity in the methane generating granular sludge by testing 4 different enrichment strategies, that included: acid (0.1 N HCl), alkali (0.2 N KOH), heat and starvation. After 14 days of incubation, the highest SO<sub>4</sub> <sup>2-</sup> removal was achieved by starved granules reaching 92.0% and was followed by heat pretreated granules (76.0%), non-pretreated granules (64.2%) and alkali pretreated granules (36.6%). The lowest SO<sub>4</sub> <sup>2-</sup> removal was reached by acid pretreated granules (22.6%). In terms of COD removal, non-pretreated granules removed the highest COD part (93.0%), while acid, heat and starved granules reached lower COD removal that was 49.2, 42.0 and 37.2%, respectively. The lower COD removal, suggests the methanogenesis inhibition. Due to low COD removal and the highest SO<sub>4</sub> <sup>2-</sup> reduction, starvation was found to be the most effective strategy for selectively enhancing the SRB activity. This pretreatment method seems to be suitable prior the continuous operation in sulfidogenic reactors using the methanogenic seed sludge.