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Immune response and efficacy of pigeon pox virus vaccine and fowl pox virus vaccine in chickens
( Niraj Kanti Shil ),( Md Siddique Rahman ),( MT Hossain ),( MT Islam ),( MM Rahman ),( KBM Saiful Islam ),( Se Yeoun Cha ),( Hyung Kwan Jang ),( Hee Jong Song ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.4
The humoral immune response of chicken vaccinated with fowl and pigeon pox virus vaccines was determined with the protective potentiality of the two vaccines in field condition of Bangladesh. Different aged Fayoumi chicks were subjected for the study. To assess the relationship with better immune response among experimental groups, the average percentage of ``take reaction`` was examined and recorded to 97.77% in group A, 93.33% in group B and 100.0% in group C. The level of immune status induced by different vaccinated group was measured by passive hemaggluti?nation(PHA) microplate test method. The mean PHA titer levels after primary vaccination were 33.06±14.13 in group A, 32.0±14.81 in group B, and 33.0±13.66 in group C. Following booster vaccination, the mean PHA titer levels in prior of challenge were increased to 55.46±14.64 in groups A and C, and 46.93±16.52 in group B. The recorded PHA titer levels of each group at two weeks after challenge were significantly increased to 106.66±31.22, 93.86±33.04 and 110.93±29.29, respectively. The PHA titer levels after vaccination and challenge were significantly increased compared to pre?vaccination titer levels(P<0.01). Although the PHA titer levels among three groups administrated different vaccine combinations in prior of challenge were significantly varied(P<0.01), it was observed that all of the vaccinated chicks were highly protected against challenge infection.
MA Hannan,MA Hossain,MT Islam 경희대학교 융합한의과학연구소 2010 Oriental Pharmacy and Experimental Medicine Vol.10 No.2
The seed powder of Abrus precatorius L. has traditionally been used as oral contraceptive agent by the women in some rural areas in Bangladesh. The present study aimed to examine the antifertility activity of A. precatorius seed extracts in experimental female rats. Finely ground seeds were extracted with aqueous acetone followed by successive partitioning with n-hexane, ethyl acetate (EtOAc), methanol (MeOH) and water. Water suspended crude seed powder, organic fractions of acetone extract and a standard contraceptive drug (Nordette®28) were separately administered orally to the female rats for 30 days. n-Hexane, EtOAc and MeOH solubles at the doses of 2, 4 and 6mg/rat/day, respectively and crude seed powder at 100 mg/rat/day exhibited 100% antifertility activity with lowest levels of serum luteinizing hormone (LH), follicle stimulating hormone (FSH)and 17β-estradiol. Histological study of ovary and uterus of these rats exhibited reduced number of developing follicles and increased number of atretic follicles in the ovary, and fewer uterine glands with shrunken morphology, reduced endometrial height, poor vascularity and compact stroma in uterus. However, the activities of serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase and the body weight of the rats remained almost unaffected in all the seed extract treated rats compared to control. These results suggest that A. precatorius seed extracts reduced the levels of serum FSH, LH and 17β-estradiol probably by affecting hypothalamic-pituitary-gonadal axis. The reduced levels of these hormones might have affected the oestrous cycle, follicular development, and subsequently the establishment of pregnancy in treated rats.