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Production of nematode free plantlets in Polianthes tuberosa using in vitro culture techniques
Kanchan B. M. Singh,Jayanthi Madhavan,Raghunath Sadhukhan,Shivani Chandra,Uma Rao,Pranab Kumar Mandal 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.5
Tuberose ( Polianthes tuberosa ) cultivation is tremendously aff ected due to Meloidogyne incognita infection. Histologicalstudy of in vitro nematode infected tuberose roots showed that the root infection initiated within 2 days post inoculation(DPI) and root gall formation occurred at 6 DPI indicating established infection on the roots. The life cycle of M. incognitain tuberose roots completed within 45 DPI evident by the formation of large number of eggs. Our study established in vitromethods like shoot tip culture and callus mediated regeneration of tubers collected from nematode infection fi elds to obtaincompletely nematode free plantlets. Tubers of diff erent varieties produced multiple shoot bud on Murashige and Skoog (MS)media containing 4 mg L −1 6-benzylaminopurine (BAP) and 0.1 mg L −1 α-naphthaleneacetic acid (NAA). Maximum numbersof plantlets were obtained for Calcutta Single (14.4 ± 2.0 per plant). Embryogenic callus was induced on MS medium supplementedwith altered concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), NAA and BAP from leaf, fl ower, root andtuber explant. The maximum callus induction was obtained on MS containing 1 mg L −1 2,4-D, 1 mg L −1 NAA and 0.5 mg L −1BAP (100%) and 1 mg L −1 2,4-D and 2.25 mg L −1 BAP (96.7%). Regeneration of tuber callus was achieved on MS with0.5 mg L −1 Kinetin (KIN) within 3 weeks. Plantlets were rooted on ½ MS with 0.5 mg L −1 Indole-3-butyric acid for 25 days. The in vitro regeneration protocol developed can thus be used for producing disease free plantlets for mass propagation.
TIETZE EXTENSION THEOREM FOR ORDERED FUZZY PRE-EXTREMALLY DISCONNECTED SPACES
Uma, M.K.,Roja, E.,Balasubramanian, G. The Youngnam Mathematical Society Korea 2008 East Asian mathematical journal Vol.24 No.3
In this paper, a new class of fuzzy topological spaces called ordered fuzzy pre-extremally disconnected spaces is introduced. Tietze extension theorem for ordered fuzzy pre-extremally disconnected spaces has been discussed as in [9] besides proving several other propositions and lemmas.
On L-fuzzy ω- basically disconnected spaces
M. Sudha,E. Roja,M. K. Uma 영남수학회 2011 East Asian mathematical journal Vol.27 No.3
In this paper L-fuzzy ω-closed and L-fuzzy ω-open sets are introduced. Also a new class of L-fuzzy topological space called L-fuzzy ω-basically disconnected space is introduced. Several characterizations and some interesting properties are also given.
ON SOMEWHAT PAIRWISE FUZZY CONTINUOUS FUNCTIONS
Uma, M.K.,Roja, E.,Balasubramanian, G. The Youngnam Mathematical Society Korea 2007 East Asian mathematical journal Vol.23 No.1
In this paper the concept of somewhat pairwise fuzzy continuous functions and somewhat pairwise fuzzy open functions are introduced. Some interesting properties of these functions are investigated besides giving some characterizations of these functions.
ON FUZZY ${\beta}-COMPACT^*$ SPACES AND FUZZY $\beta$-FILTERS
Uma, M.K.,Roja, E.,Balasubramanian, G. The Youngnam Mathematical Society Korea 2007 East Asian mathematical journal Vol.23 No.2
In this paper we introduce the concept of fuzzy ${\beta}-compact^*$ spaces. Besides giving some interesting properties of fuzzy ${\beta}-compact^*$ spaces we also give a characterization on fuzzy $\beta$-compact spaces by making use of newly introduced concept of fuzzy $\beta$-filters.
M. Muthusamy,S. Uma,S. Backiyarani,M. S. Saraswathi 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.5
Long non-coding RNAs (LncRNAs) are one of the many layers of transcription in higher plants. LncRNAs are responsive to biotic and abiotic stresses and regulate genes. In our study, we have identified 905 novel lncRNAs from 8471 drought-responsive, novel transcripts of RNASeq reads from two banana cultivars, a drought-tolerant cv. ‘Saba’ (ABB) and -susceptible cv. ‘Grand Naine’ (AAA). Of these 905 lncRNAs, 75 (8.3 %) transcripts were natural antisense RNAs (NATs) and 2 transcripts identified as precursors of microRNA-miR156 and miR166. Among the 905 identified lncRNAs, 216, 150 and 279, 164 lncRNAs were induced and reduced to drought stress, respectively, in tolerant and susceptible in comparison to their equivalent controls. The remaining 22 lncRNA of tolerant cultivars was not regulated by drought stress. Of the 882 drought-responsive lncRNAs, 44 new lncRNAs were identified as induced. Musa lncRNAs were unevenly distributed in 11 chromosomes of Musa acuminata and no lncRNAs were found in chromosome-9 of drought-tolerant cultivar. The average lengths of lncRNAs were 683 nucleotides (nt). Drought-responsive differential expression of lncRNAs was found between +8.11585- and -4.04311-fold. Around 7.9 % of the identified lncRNAs were decoys of 85 conserved microRNAs. These findings will lay a basic platform for effective strategic planning of developing drought-resilient crop varieties.
Tietze extension theorem for ordered fuzzy pre-extremally disconnected spaces
M. K. Uma,E. Roja,G. Balasubramanian 영남수학회 2008 East Asian mathematical journal Vol.24 No.3
In this paper, a new class of fuzzy topological spaces called ordered fuzzy pre-extremally disconnected spaces is introduced. Tietze extension theorem for ordered fuzzy pre-extremally disconnected spaces has been discussed as in [9] besides proving several other propositions and lemmas.
Development of new tool using formal psychological assessment for depression diagnosis
( M. Yohapriyaa ),( Dr. M. Uma ) 한국감성과학회 2021 추계학술대회 Vol.2021 No.0
Major Depressive Disorder (MDD) can include a variety of symptoms. It's critical to distinguish between different forms of MDEs in order to get the best therapy. The goal of this study is to offer a new tool for assessing depression in people with major depressive disorder in order to address some of the shortcomings of current rating scales. The proposed method examines all of the clinical characteristics of various MDEs and provides qualitative information, allowing individuals with the same score but distinct symptoms and degree of psychopathology to be distinguished. The proposed approach is build based on the framework called Formal Psychological Assessment (FPA). Psychological assessment is characterized as a lengthy process of gathering, analyzing, and processing data. FPA aims to enhance this process by establishing a formal framework within which to develop assessment instruments. FPA provides relationships between assessment tool items and a set of diagnostic criteria for a certain clinical condition. Three commonly used measures for self-evaluation of depression were chosen. The DSM-5, literature, and Seligman and Beck's theories have been used to construct diagnostic criteria for major depressive disorder. A Boolean matrix was created, using 69 questions in the rows and 15 chosen indications in the columns. A value of 1 in a cell of the matrix indicates that the associated item examines the specified characteristic. The proposed tool provides information regarding a patient's most likely diagnostic configuration.
Development of new tool using formal psychological assessment for depression diagnosis
( M. Yohapriyaa ),( M. Uma ) 한국감성과학회 2021 한국감성과학회 국제학술대회(ICES) Vol.2021 No.-
Major Depressive Disorder (MDD) can include a variety of symptoms. It's critical to distinguish between different forms of MDEs in order to get the best therapy. The goal of this study is to offer a new tool for assessing depression in people with major depressive disorder in order to address some of the shortcomings of current rating scales. The proposed method examines all of the clinical characteristics of various MDEs and provides qualitative information, allowing individuals with the same score but distinct symptoms and degree of psychopathology to be distinguished. The proposed approach is build based on the framework called Formal Psychological Assessment (FPA). Psychological assessment is characterized as a lengthy process of gathering, analyzing, and processing data. FPA aims to enhance this process by establishing a formal framework within which to develop assessment instruments. FPA provides relationships between assessment tool items and a set of diagnostic criteria for a certain clinical condition. Three commonly used measures for self-evaluation of depression were chosen. The DSM-5, literature, and Seligman and Beck's theories have been used to construct diagnostic criteria for major depressive disorder. A Boolean matrix was created, using 69 questions in the rows and 15 chosen indications in the columns. A value of 1 in a cell of the matrix indicates that the associated item examines the specified characteristic. The proposed tool provides information regarding a patient's most likely diagnostic configuration.
Slightly Fuzzy Continuous mappings
M. Sudha,E. Roja,M. K. Uma 영남수학회 2009 East Asian mathematical journal Vol.25 No.2
In this paper slightly fuzzy continuous mappings is introduced and some interesting properties and characterizations of slightly fuzzy continuous mappings are discussed.