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Kim, Jinsook,Song, Insil,Jo, Ara,Shin, Joo-Ho,Cho, Hana,Eoff, Robert L.,Guengerich, F. Peter,Choi, Jeong-Yun American Chemical Society 2014 Chemical research in toxicology Vol.27 No.10
<P/><P>DNA polymerase (pol) ι is the most error-prone among the Y-family polymerases that participate in translesion synthesis (TLS). Pol ι can bypass various DNA lesions, e.g., <I>N</I><SUP>2</SUP>-ethyl(Et)G, <I>O</I><SUP>6</SUP>-methyl(Me)G, 8-oxo-7,8-dihydroguanine (8-oxoG), and an abasic site, though frequently with low fidelity. We assessed the biochemical effects of six reported genetic variations of human pol ι on its TLS properties, using the recombinant pol ι (residues 1–445) proteins and DNA templates containing a G, <I>N</I><SUP>2</SUP>-EtG, <I>O</I><SUP>6</SUP>-MeG, 8-oxoG, or abasic site. The Δ1–25 variant, which is the <I>N</I>-terminal truncation of 25 residues resulting from an initiation codon variant (c.3G > A) and also is the formerly misassigned wild-type, exhibited considerably higher polymerase activity than wild-type with Mg<SUP>2+</SUP> (but not with Mn<SUP>2+</SUP>), coinciding with its steady-state kinetic data showing a ∼10-fold increase in <I>k</I><SUB>cat</SUB>/<I>K</I><SUB>m</SUB> for nucleotide incorporation opposite templates (only with Mg<SUP>2+</SUP>). The R96G variant, which lacks a R96 residue known to interact with the incoming nucleotide, lost much of its polymerase activity, consistent with the kinetic data displaying 5- to 72-fold decreases in <I>k</I><SUB>cat</SUB>/<I>K</I><SUB>m</SUB> for nucleotide incorporation opposite templates either with Mg<SUP>2+</SUP> or Mn<SUP>2+</SUP>, except for that opposite <I>N</I><SUP>2</SUP>-EtG with Mn<SUP>2+</SUP> (showing a 9-fold increase for dCTP incorporation). The Δ1–25 variant bound DNA 20- to 29-fold more tightly than wild-type (with Mg<SUP>2+</SUP>), but the R96G variant bound DNA 2-fold less tightly than wild-type. The DNA-binding affinity of wild-type, but not of the Δ1–25 variant, was ∼7-fold stronger with 0.15 mM Mn<SUP>2+</SUP> than with Mg<SUP>2+</SUP>. The results indicate that the R96G variation severely impairs most of the Mg<SUP>2+</SUP>- and Mn<SUP>2+</SUP>-dependent TLS abilities of pol ι, whereas the Δ1–25 variation selectively and substantially enhances the Mg<SUP>2+</SUP>-dependent TLS capability of pol ι, emphasizing the potential translational importance of these pol ι genetic variations, e.g., individual differences in TLS, mutation, and cancer susceptibility to genotoxic carcinogens.</P>
이희원(HeeWon Lee),정다혜(DaHye Jeong),주다운(DaWoon Joo),이진숙(JinSook Lee) 한국색채학회 2016 한국색채학회 학술대회 Vol.2016 No.5
본 연구에서는 학습공간에서의 주의집중력에 따른 색온도와 조도변화에 대하여 인체의 생리적인 반응을 알아보고자 하였다. 연구의 방법으로는 FAIR 주의집중력검사를 통하여 주의집중력이 낮은 그룹과 높은 그룹을 분류하였다. 학습공간으로 실물대 Mock-up을 조성하였으며, 조명환경의 실험변인은 직접조명을 사용하여 색온도 3000 K, 6000K, 조도는 60 ㏓, 600 ㏓이다. 10%-20% 국제표준전극 부착법을 이용하여 각 부위에 전극을 부착한 후 뇌파를 측정하였으며, 연구의 결과는 다음과 같다. 색온도 3000K 600 lx, 6000K 600 lx 의 조명환경은 주의집중력이 낮은 그룹과 높은 그룹 모두 주의집중할 수 있는 조명환경이었으며, 밝은 조명환경일수록 주의집중함을 알 수 있었다.