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- 저자 : ( Jianjie Gao ) (검색결과 3 건)
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Interpenetration Enhancing of Chitosan-PEGLM Double Network (DN) Hydrogel and Its Properties
Yiting Xu,Jianjie Xie,Hui Gao,Ying Cao,Min Chen,Yanling Liu,Birong Zeng,Feng-Chih Chang,Lizong Dai 한국고분자학회 2015 Macromolecular Research Vol.23 No.1
A series of polylactic acid-polyethylene glycol-polylactic acid diacrylate macromers (PEGLM) were synthesized,and a novel chitosan-PEGLM double network (DN) hydrogel was further successfully fabricated by thesequential interpenetrating technology with highly cross-linked chitosan as the rigid component and PEGLM as theflexible component. Their structures and components were characterized by 1H NMR, FTIR, and XRD. Their fracturemorphology was investigated by SEM. It was interesting to found that the compressive strength of hydrogel woundreach its maximum value when the chitosan content was 10%, no matter what the molecular weight of PEGLM componentwas. What’s more, we found that the compressive strength of 6KL7 could reach 1.07 MPa at gel state, whosePEG segment molecular weight was 6,000, and polymerization degree of PLA was 7. The effects of glutaraldehyderatio, polymerization degree of PLA, and molecular weight of PEG segment on the mechanical strength of DNhydrogels were also discussed in this article. To further strengthen DN hydrogels, the double network-linear (DNL)hydrogels were fabricated by introducing linear poly(vinyl alcohol) (PVA) into the DN hydrogels. The DN-Lhydrogels exhibited better mechanical properties, with the compressive strength up to 1.45 MPa. These hydrogelsmay have prospective applications in the fields of wound dressing, artificial cartilage and tissue engineering scaffoldmaterials which require high mechanical properties.
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Shuai Hao,Wuguo Tian,Jianjie Zhao,Yi Chen,Xiaohua Zhang,Bo Gao,Yujun He,Donglin Luo 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4
Purpose: Real-time detection and intervention can be used as potential measures to markedly decrease breast cancer mortality. Assessment of circulating tumor DNA (ctDNA) may offer great benefits for the management of breast cancer over time. However, the use of ctDNA to predict the effectiveness of neoadjuvant treatment and recurrence of breast cancer has rarely been studied. Methods: We prospectively recruited 31 breast cancer patients with 4 subtypes. Three time points were set in this study, including before any therapy (C1), during surgery (T), and six months after surgery (C2). We collected peripheral blood samples from all 31 patients at C1, tumor tissue from all 31 patients at T, and peripheral blood samples from 25 patients at C2. Targeted 727-gene panel sequencing was performed on ctDNA from all blood samples and tissue DNA from all tissue samples. Somatic mutations were detected and analyzed using a reference standard pipeline. Statistical analysis was performed to identify possible associations between ctDNA profiles and clinical outcomes. Results: In total, we detected 159, 271, and 70 somatic mutations in 30 C1 samples, 31 T samples, and 12 C2 samples, respectively. We identified specific genes, such as PIK3CA, TP53, and KMT2C, which were highly mutated in the tissue samples. Furthermore, mutated KMT2C observed in ctDNA of the C2 samples may be an indicator of breast cancer recurrence. Conclusion: Our study highlights the potential of ctDNA analysis at different timepoints for assessing tumor progression and treatment effectiveness, as well as prediction of breast cancer recurrence.
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( Bo Wang ),( Ying Yan ),( Jing Xu ),( Xiaoyan Fu ),( Hongjuan Han ),( Jianjie Gao ),( Zhenjun Li ),( Lijuan Wang ),( Yongsheng Tian ),( Rihe Peng ),( Quanhong Yao ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and 40°C. It exhibited K<sub>m</sub> and V<sub>max</sub> values of 0.565 mmol l<sup>-1</sup> and 1.51 μmol l<sup>-1</sup> min<sup>-1</sup> for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.
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