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In Vitro Inhibitory Effect of Licoricidin on Human Cytochrome P450s
( Sunju Kim ),( Heungchan O ),( Jeong Ah Kim ),( Seung Ho Lee ),( Sangkyu Lee ) 한국질량분석학회 2014 Mass spectrometry letters Vol.5 No.3
Licoricidin isolated from Glycyrrhiza uralensis is known to have anticancer, anti-nephritic, anti-Helicobacter pylori, and antibacterial effects. In this study, a cocktail probe assay and liquid chromatography-tandem mass spectrometry (LC-MS/ MS) were used to investigate the modulating effect of licoricidin on cytochrome P450 (CYP) enzymes in human liver microsomes. When licoricidin was incubated at 0-25 μM with CYP probes for 60 min at 37oC, it showed potent inhibitory effects on CYP2B6-catalyzed bupropion hydroxylation and CYP2C9-catalyzed diclofenac 4’-hydroxylation with half maximal inhibitory concentration (IC50) values of 3.4 and 4.0 μM, respectively. The inhibition mode of licoricidin was revealed as competitive, dose-dependent, and non-time-dependent, and following the pattern of Lineweaver-Burk plots. The inhibitory effect of licoricidin has been confirmed in human recombinant cDNA-expressed CYP2B6 and 2C9 with IC50 values of 4.5 and 0.73 μM, respectively. In conclusion, this study has shown the potent inhibitory effect of licoricidin on CYP2B6 and CYP2C9 activity could be important for predicting potential herb-drug interactions with substrates that mainly undergo CYP2B- and CYP2C9- mediated metabolism.
In Vitro Inhibitory Effect of Licoricidin on Human Cytochrome P450s
Kim, Sunju,O, Heungchan,Kim, Jeong Ah,Lee, Seung Ho,Lee, Sangkyu Korean Society for Mass Spectrometry 2014 Mass spectrometry letters Vol.5 No.3
Licoricidin isolated from Glycyrrhiza uralensis is known to have anticancer, anti-nephritic, anti-Helicobacter pylori, and antibacterial effects. In this study, a cocktail probe assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the modulating effect of licoricidin on cytochrome P450 (CYP) enzymes in human liver microsomes. When licoricidin was incubated at $0-25{\mu}m$ with CYP probes for 60 min at $37^{\circ}C$, it showed potent inhibitory effects on CYP2B6-catalyzed bupropion hydroxylation and CYP2C9-catalyzed diclofenac 4'-hydroxylation with half maximal inhibitory concentration ($IC_{50}$) values of 3.4 and $4.0{\mu}m$, respectively. The inhibition mode of licoricidin was revealed as competitive, dose-dependent, and non-time-dependent, and following the pattern of Lineweaver-Burk plots. The inhibitory effect of licoricidin has been confirmed in human recombinant cDNA-expressed CYP2B6 and 2C9 with $IC_{50}$ values of 4.5 and $0.73{\mu}m$, respectively. In conclusion, this study has shown the potent inhibitory effect of licoricidin on CYP2B6 and CYP2C9 activity could be important for predicting potential herb-drug interactions with substrates that mainly undergo CYP2B- and CYP2C9-mediated metabolism.
카카오톡 API를 이용한 Django 기반 챗봇 시스템
고흥찬,김민수,이솔비,이형우,Ko, Heungchan,Kim, Minsu,Lee, Solbi,Lee, Hyung-Woo 한국사물인터넷학회 2018 한국사물인터넷학회 논문지 Vol.4 No.1
본 논문은 대학생들이 소속 대학 내 주요 정보를 손쉽고 빠르게 검색할 수 있도록 KakaoTalk API를 활용하여 장고 프레임워크를 이용하여 챗봇 시스템을 개발하였다. 특정 정보만을 제공하는 기존 챗봇 시스템과는 달리 본 연구에서 개발한 챗봇은 대학 내 주요 정보 뿐만아니라 날씨, 유투브, 네이버 실시간 랭킹 검색 및 언어 번역 등 다양한 형태의 사용자 질의에 대해 자동으로 검색 결과를 제공한다. AWS Ubuntu 서버내에 Apache, Python 및 Django를 이용하여 모듈을 개발하였고 KakaoTalk API 및 BeautifulSoup 등을 이용하여 카카오톡 서버와 통신하여 사용자 질의에 대해 자동으로 응답하는 챗봇 시스템을 개발하였다. 본 연구에서 개발한 시스템에 대해서는 향후 대학 입시 정보 홍보 및 선거 홍보 시스템 등에 적용할 수 있을 것으로 기대된다. In this paper, we developed a chatbot system using the Django framework using the KakaoTalk API so that college students can easily search for important information in their university. Unlike existing chatbot systems that provide only specific information, the chatbot developed in this research automatically provides search results for various types of user queries such as weather, YouTube, Naver real-time ranking search and language translation as well as important information within their own university. We developed a module using Apache, Python and Django in AWS Ubuntu server and developed a chatbot system that automatically responds to user queries by communicating with KakaoTalk server using KakaoTalk API and BeautifulSoup. The system developed in this study is expected to be applicable to the future university entrance information promotion and election promotion system.