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A method using artificial neural networks to morphologically assess mouse blastocyst quality
Matos, Felipe Delestro,Rocha, Jose Celso,Nogueira, Marcelo Fabio Gouveia Korean Society of Animal Sciences and Technology 2014 한국축산학회지 Vol.56 No.4
Background: Morphologically classifying embryos is important for numerous laboratory techniques, which range from basic methods to methods for assisted reproduction. However, the standard method currently used for classification is subjective and depends on an embryologist's prior training. Thus, our work was aimed at developing software to classify morphological quality for blastocysts based on digital images. Methods: The developed methodology is suitable for the assistance of the embryologist on the task of analyzing blastocysts. The software uses artificial neural network techniques as a machine learning technique. These networks analyze both visual variables extracted from an image and biological features for an embryo. Results: After the training process the final accuracy of the system using this method was 95%. To aid the end-users in operating this system, we developed a graphical user interface that can be used to produce a quality assessment based on a previously trained artificial neural network. Conclusions: This process has a high potential for applicability because it can be adapted to additional species with greater economic appeal (human beings and cattle). Based on an objective assessment (without personal bias from the embryologist) and with high reproducibility between samples or different clinics and laboratories, this method will facilitate such classification in the future as an alternative practice for assessing embryo morphologies.
A method using artificial neural networks to morphologically assess mouse blastocyst quality
Felipe Delestro Matos,José Celso Rocha,Marcelo Fábio Gouveia Nogueira 한국축산학회 2014 한국축산학회지 Vol.56 No.5
Background: Morphologically classifying embryos is important for numerous laboratory techniques, which rangefrom basic methods to methods for assisted reproduction. However, the standard method currently used forclassification is subjective and depends on an embryologist’s prior training. Thus, our work was aimed atdeveloping software to classify morphological quality for blastocysts based on digital images. Methods: The developed methodology is suitable for the assistance of the embryologist on the task of analyzingblastocysts. The software uses artificial neural network techniques as a machine learning technique. These networks analyzeboth visual variables extracted from an image and biological features for an embryo. Results: After the training process the final accuracy of the system using this method was 95%. To aid theend-users in operating this system, we developed a graphical user interface that can be used to produce a qualityassessment based on a previously trained artificial neural network. Conclusions: This process has a high potential for applicability because it can be adapted to additional specieswith greater economic appeal (human beings and cattle). Based on an objective assessment (without personal biasfrom the embryologist) and with high reproducibility between samples or different clinics and laboratories, thismethod will facilitate such classification in the future as an alternative practice for assessing embryo morphologies.
( Felipe Delestro Matos ),( Jose Celso Rocha ),( Marcelo Fabio Gouveia Nogueira ) 한국동물자원과학회(구 한국축산학회) 2014 한국축산학회지 Vol.56 No.15
Background: Morphologically classifying embryos is important for numerous laboratory techniques, which range from basic methods to methods for assisted reproduction. However, the standard method currently used for classification is subjective and depends on an embryologist’s prior training. Thus, our work was aimed at developing software to classify morphological quality for blastocysts based on digital images. Methods: The developed methodology is suitable for the assistance of the embryologist on the task of analyzing blastocysts. The software uses artificial neural network techniques as a machine learning technique. These networks analyze both visual variables extracted from an image and biological features for an embryo. Results: After the training process the final accuracy of the system using this method was 95%. To aid the end-users in operating this system, we developed a graphical user interface that can be used to produce a quality assessment based on a previously trained artificial neural network. Conclusions: This process has a high potential for applicability because it can be adapted to additional species with greater economic appeal (human beings and cattle). Based on an objective assessment (without personal bias from the embryologist) and with high reproducibility between samples or different clinics and laboratories, this method will facilitate such classification in the future as an alternative practice for assessing embryo morphologies.