http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Cell-Interactive Polymers for Tissue Engineering
Lee, Kuen Yong,Mooney, David J. The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2
Tissue engineering is one exciting approach to treat patients who need a new organ or tissue. A critical element in this approach is the polymer scaffold, as it provides a space for new tissue formation and mimics many roles of natural extra-cellular matrices. In this review, we describe several design parameters of polymer matrices that can significantly affect cellular behavior, as well as various polymers which are frequently used to date or potentially useful in many tissue engineering applications. Interactions between cells and polymer scaffolds, including specific receptor-ligand interactions, physical and degradation feature of the scaffolds, and delivery of soluble factors, should be considered in the design and tailoring of appropriate polymer matrices to be used in tissue engineering applications, as these interactions control the function and structure of engineered tissues.
Huebsch, Nathaniel,Kearney, Cathal J.,Zhao, Xuanhe,Kim, Jaeyun,Cezar, Christine A.,Suo, Zhigang,Mooney, David J. National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.27
<P>Biological systems are exquisitely sensitive to the location and timing of physiologic cues and drugs. This spatiotemporal sensitivity presents opportunities for developing new therapeutic approaches. Polymer-based delivery systems are used extensively for attaining localized, sustained release of bioactive molecules. However, these devices typically are designed to achieve a constant rate of release. We hypothesized that it would be possible to create digital drug release, which could be accelerated and then switched back off, on demand, by applying ultrasound to disrupt ionically cross-linked hydrogels. We demonstrated that ultrasound does not permanently damage these materials but enables nearly digital release of small molecules, proteins, and condensed oligonucleotides. Parallel in vitro studies demonstrated that the concept of applying temporally short, high-dose “bursts” of drug exposure could be applied to enhance the toxicity of mitoxantrone toward breast cancer cells. We thus used the hydrogel system in vivo to treat xenograft tumors with mitoxantrone, and found that daily ultrasound-stimulated drug release substantially reduced tumor growth compared with sustained drug release alone. This approach of digital drug release likely will be applicable to a broad variety of polymers and bioactive molecules, and is a potentially useful tool for studying how the timing of factor delivery controls cell fate in vivo<I>.</I></P>
Kim, Jaeyun,Li, Weiwei Aileen,Sands, Warren,Mooney, David J. American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.11
<P/><P>The <I>in vivo</I> enrichment of dendritic cells (DCs) in implanted macroporous scaffolds is an emerging strategy to modulate the adaptive immune system. The pore architecture is potentially one of the key factors in controlling enrichment of DCs. However, there have been few studies examining the effects of scaffold pore structure on <I>in vivo</I> DC enrichment. Here we present the effects of surface porosity, pore size, and pore volume of macroporous poly(lactide-<I>co</I>-glycolide) (PLG) scaffolds encapsulating granulocyte macrophage colony-stimulating factor (GM-CSF), an inflammatory chemoattractant, on the <I>in vivo</I> enrichment of DCs. Although <I>in vitro</I> cell seeding studies using PLG scaffolds without GM-CSF showed higher cell infiltration in scaffolds with higher surface porosity, <I>in vivo</I> results revealed higher DC enrichment in GM-CSF loaded PLG scaffolds with lower surface porosity despite a similar level of GM-CSF released. The diminished compressive modulus of high surface porosity scaffolds compared to low surface porosity scaffolds lead to the significant shrinkage of these scaffolds <I>in vivo,</I> suggesting that the mechanical strength of scaffolds was critical to maintain a porous structure <I>in vivo</I> for accumulating DCs. The pore volume was also found to be important in total number of recruited cells and DCs <I>in vivo.</I> Varying the pore size significantly impacted the total number of cells, but similar numbers of DCs were found as long as the pore size was above 10–32 μm. Collectively, these results suggested that one can modulate <I>in vivo</I> enrichment of DCs by altering the pore architecture and mechanical properties of PLG scaffolds.</P>
Shear-reversibly Crosslinked Alginate Hydrogels for Tissue Engineering
Park, Honghyun,Kang, Sun-Woong,Kim, Byung-Soo,Mooney, David J.,Lee, Kuen Yong WILEY-VCH Verlag 2009 Macromolecular bioscience Vol.9 No.9
<P>Injectable delivery vehicles in tissue engineering are often required for successful tissue formation in a minimally invasive manner. Shear-reversibly crosslinked hydrogels, which can recover gel structures from shear-induced breakdown, can be useful as an injectable, because gels can flow as a liquid when injected but re-gel once placed in the body. In this study, injectable and shear-reversible alginate hydrogels were prepared by combination crosslinking using cell-crosslinking and ionic crosslinking techniques. The addition of a small quantity of calcium ions decreased the number of cells that were required to form cell-crosslinked hydrogels without changing the shear reversibility of the system. The physical properties and gelation behavior of the gels were dependent on the concentration of both the cells and the calcium ions. We found that gels crosslinked by combination crosslinking methods were effective to engineer cartilage tissues in vivo. Using both ionic and cell-crosslinking methods to control the gelation behavior may allow the design of novel injectable systems that can be used to deliver cells and other therapeutics for minimally invasive therapy, including tissue engineering.</P><P> <img src='wiley_img/16165187-2009-9-9-MABI200800376-gra001.gif' alt='wiley_img/16165187-2009-9-9-MABI200800376-gra001'> </P> <B>Graphic Abstract</B> <P>Injectable alginate hydrogels with shear-reversible gelation behavior can be prepared by combination crosslinking using cell-crosslinking and ionic crosslinking techniques. The addition of a small quantity of calcium ions can decrease the number of cells which are required to form cell-crosslinked hydrogels without changing the shear reversibility. Gels are useful to engineer cartilage tissues in vivo. <img src='wiley_img/16165187-2009-9-9-MABI200800376-content.gif' alt='wiley_img/16165187-2009-9-9-MABI200800376-content'> </P>
Tissue Engineering of Smooth Muscle under a Mechanically Dynamic Condition
( Byung Soo Kim ),( Sung In Jeong ),( Seung Woo Cho ),( Janeta Nikolovski ),( David J. Mooney ),( Soo Hong Lee ),( O Ju Jeon ),( Tae Wan Kim ),( Sang Hyun Lim ),( Yoo Sun Hong ),( Cha Yong Choi ),( Yo 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.6