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      • KCI등재

        Bioguided Fractionation, and Antioxidant, Antiproliferative, and Anti-Inflammatory Activity of Annona cacans Warm

        Carla Roberta Ferreira Volobuff,Milena Menezes Correˆa Pederiva,Roseane Soares Ramos Benites,Camila Juliane Lima,Eliana Janet Sanjinez Argandon˜a,Clau´dia Andrea Lima Cardoso,Zefa Valdevina Pereira,An 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.10

        This study evaluated to determine the phenolic and flavonoids contents, and antioxidant, anti-inflammatory, and antiproliferative activity of the hydromethanolic extracts of the leaves, pulp, and seeds of Annona cacans. The isolation and structural identification of the constituent acetogenin, phenolic acid, and flavonoids were also reported. Antioxidant capacity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ethylbenzothiazoline-6-sulfonic acid (ABTS), and β-carotene/linoleic acid methods. Cell proliferation was determined by spectrophotometric quantification of the cellular protein content using a sulforhodamine B assay. Anti-inflammatory activity was evaluated in paw edema model, to myeloperoxidase (MPO) activity induced by carrageenan in mice. Fractionation resulted in the isolation of one acetogenin (annoreticuin-9-one), two flavonoids (quercetin-3-O-β-glucoside-6-O-α-rhamnoside and kaempferol-3-O-β-glucoside), and one phenolic acid (p-coumaric acid). The pulp extract presented potent antioxidant activities by the DPPH (IC50 = 44.08 μg/mL) and ABTS (IC50 = 39.32 μg/mL) methods, as well as high contents of phenols (618.95 mg GA/g) and flavonoids (477.35 mg QE/g). The bioguided fractionation demonstrated that the ethyl acetate fraction of the pulp extract and annoreticuin-9-one showed potent antiproliferative activity against ovarian cancer (GI50 = 6.4 μg/mL). The anti-inflammatory activity demonstrated significant inhibition of edema compared to the control group in 2 and 4 h; in addition, the extracts inhibited the increase in MPO activity after 6 h, when compared to the DEX and control groups. For the first time, this study demonstrated antioxidant, anti-inflammatory, and antiproliferative activity, as well as compounds isolated, suggesting that A. cacans could also be potential sources for prevention of cancer and other diseases associated with oxidative stress.

      • KCI등재

        Numerical Analysis on Distortional Failure of Cold-Formed Steel Hat-Section Beams Under Non-uniform Bending

        Carla de Amorim Lana Dib,Guilherme Henrique dos Santos Ramos,Gregório Sandro Vieira 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.5

        Cold-formed steel (CFS) members stand out among steel structures notably due to their lightness, structural efficiency (high strength-to-weight ratio) and versatility, however, given their high width-to-thickness ratio, are highly susceptible to instability phenomena (buckling). The objective of this research is to analyze the structural behavior of cold-formed steel hat-section beams under non-uniform bending about the major and minor-axis, regarding the risks of distortional failure. Through the Generalized Beam Theory, using the computational program GBTUL, the geometries where the distortional failure is predominant were selected and through the computational program ABAQUS, a shell finite element model was developed to perform the buckling and post-buckling analysis on the selected elements. The results obtained from the developed model—which include critical buckling moments, failure moments, equilibrium paths, deformed configurations and collapse mechanisms—show that the developed model adequately simulates the distortional buckling and post-buckling behavior of the considered CFS beams and demonstrate how loading affects such behaviors.

      • SCOPUSKCI등재

        Ambient Air Waste Sorting Facilities Could Be a Source of Antibiotic Resistant Bacteria

        ( Ana Calheiros ),( Joana Santos ),( Carla Ramos ),( Marta Vasconcelos ),( Paulo Fernandes ) 한국미생물 · 생명공학회 2021 한국미생물·생명공학회지 Vol.49 No.3

        The antimicrobial resistance of Staphylococcus spp. and Gram negative strains present in air samples from waste sorting facilities was assessed. Phenotypic studies have revealed a high percentage of strains of Staphylococcus spp. resistant to methicillin. Genotypically and by RT-PCR, it was found that the mecA gene usually associated with methicillin resistance was present in 8% of the Staphylococcus strains isolated. About 30% of the Gram negative strains from the same samples also displayed resistance to meropenem and 79% of these were resistant to multiple antibiotics from different classes, namely cephalosporins and β- lactams. The results suggest that in professional activities with high levels of exposure to biological agents, the quantification and identification of the microbial flora in the work environment, with the determination of the presence of potential agents displaying multi-resistances is of relevance to the risk assessment. The personal protection of workers is particularly important relevance in these cases, since many of the strains that exhibit multi-resistance are potential opportunistic agents.

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