NANOBIOTECHNOLOGY: AN INTERFACE BETWEEN NANOTECHNOLOGY AND BIOTECHNOLOGY

RASHID AMIN,SIEUN HWANG,박성하 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.2

Nanotechnology is one of the most important emerging fields of science in this century. It deals with designing, construction, investigation, and utilization of systems at the nanoscale. Another interesting research discipline of current day is biotechnology, which gives us a way to understand biological system and to utilize our knowledge for industrial manufacturing. Nanobiotechnology lies at the interface of these two research fields. It exploits nanotechnology and biotechnology to analyze and create nanobiosystems to meet a wide variety of challenges and develops a wide range of applications.

Biological green synthesis of gold and silver nanoparticles

Sur, Ujjal Kumar Techno-Press 2014 Advances in nano research Vol.2 No.3

Nanomaterials synthesized by natural bioresources such as microorganisms, animals and plants in nature can also be synthesized in laboratories even on large scale. This is considered as an attractive prospect for eco-friendly or so-called green synthesis. Development of eco-friendly synthesis of biocompatible nanoparticles and their potential biomedical applications introduces the concept of nanobiotechnology. The lower cost and lesser side effects as compare to chemical methods of synthesis are the main advantages of biosynthesis. This review article demonstrates the role of various biological resources e.g. bacteria, fungi, actinomycetes, plant leaves, fruits and honey as well as animal tissues for the synthesis of nanoparticles mainly gold and silver with an overview of their potential applications.

Green synthesis of fluorescent carbon nanoparticles from Lychee (Litchi chinensis) plant

Vidhi Chaudhary,Anil Kumar Bhowmick 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.8

Fluorescent carbon nanoparticles (CNPs) were prepared from waste lychee peel by hydrothermal process using green chemistry. These CNPs were characterized by UV-vis absorption, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction. Their morphology was analyzed by field emission scanning electron microscopy. Spherical carbon nanoparticles with a particle size ranging from 40-70 nm were observed. FTIR data indicated that these CNPs were functionalized with hydroxyl and carboxylic or carbonyl group. The maximum fluorescence intensity for these CNPs was observed on the excitation wavelength at 365 nm with emission maxima centered at 450 nm. These particles exhibited excitation wavelength dependent fluorescence emission spectra. CNPs were found to be highly fluorescent and exhibited high water solubility. The band gap was estimated to be 3.8 eV. Therefore, as prepared CNPs would be useful in bioimaging, biolabeling and in the other applications of nanobiotechnology.

Designer nanoparticle: nanobiotechnology tool for cell biology

Thimiri Govinda Raj Deepak B.,Khan Niamat Ali 나노기술연구협의회 2016 Nano Convergence Vol.3 No.22

This article discusses the use of nanotechnology for subcellular compartment isolation and its application towards subcellular omics. This technology review significantly contributes to our understanding on use of nanotechnology for subcellular systems biology. Here we elaborate nanobiotechnology approach of using superparamagnetic nanoparticles (SPMNPs) optimized with different surface coatings for subcellular organelle isolation. Using pulse-chase approach, we review that SPMNPs interacted differently with the cell depending on its surface functionalization. The article focuses on the use of functionalized-SPMNPs as a nanobiotechnology tool to isolate high quality (both purity and yield) plasma membranes and endosomes or lysosomes. Such nanobiotechnology tool can be applied in generating subcellular compartment inventories. As a future perspective, this strategy could be applied in areas such as immunology, cancer and stem cell research.

Nanobiotechnology for diagnosis and vaccine against Viral Disease X in animal and human

Daesub Song 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

ADVANCES IN F0F1-ATP SYNTHASE BIOLOGICAL PROTEIN NANOMOTOR: FROM MECHANISMS AND STRATEGIES TO POTENTIAL APPLICATIONS

A. R. KHATAEE,H. R. KHATAEE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2009 NANO Vol.4 No.2

Movement and shape changes are fundamental aspects of all living organisms. This biological motility results from the biological nanomotors, in particular protein nanomotors. Cells contain a variety of protein nanomotors that rotate (e.g., F0F1-ATP synthase or bacterial flagellar motors) or move in a linear fashion (e.g., the kinesin, myosin and dynein motors). F0F1-ATP synthase is one of the ideal nanomotors or energy providing systems for micro/nanomachines because of its small size, smart and perfect structure, and ultra-high energy transfer efficiency. Therefore, in this paper, we have reviewed the structure, mechanism, and potential applications of the F0F1-ATP synthase nanomotor. In all organisms, the F0F1-ATP synthase consists of two distinct nanomotors, F0 and F1. The F0 moiety is embedded in the membrane and is a detergent soluble unit while the F1 moiety protrudes from the membrane and is a water soluble unit. F0F1-ATP synthase operates as two stepper motor/generators coupled by a common shaft and an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360° rotary motion of subunits. F0F1-ATP synthase nanomotor may enable the creation of a new class of sensors, mechanical force transducers, actuators, and nanomechanical devices. Thus, the F0F1-ATP synthase nanomotor field has expanded into a wide variety of science.

Multiscale Biofluidic and Nanobiotechnology Approaches for Treating Sepsis in Extracorporeal Circuits

강주헌 한국바이오칩학회 2020 BioChip Journal Vol.14 No.1

Infectious diseases and their pandemics periodically attract public interests due to difficulty in treating the patients and the consequent high mortality. Sepsis caused by an imbalanced systemic inflammatory response to infection often leads to organ failure and death. The current therapeutic intervention mainly includes “the sepsis bundles,” antibiotics (antibacterial, antiviral, and antifungal), intravenous fluids for resuscitation, and surgery, which have significantly improved the clinical outcomes in past decades; however, the patients with fulminant sepsis are still in desperate need of alternative therapeutic approaches. One of the potential supportive therapies, extracorporeal blood treatment, has emerged and been developed for improving the current therapeutic efficacy. Here, I overview how the treatment of infectious diseases has been assisted with the extracorporeal adjuvant therapy and the potential utility of various nanobiotechnology and microfluidic approaches for developing new auxiliary therapeutic methods.

특허정보를 활용한 나노바이오 융합기술의 특성 분석

노현정 ( Hyun Joung No ),임효정 ( Hyojeong Lim ) 한국지식재산연구원 2009 지식재산연구 Vol.4 No.3

서로 다른 산업이나 기술 경계간의 접점에서 혁신이 발생할 때 융합이라는 현상이 나타나는데, 이는 같은 종류의 산업이나 기술들 사이에서 발생하는 혁신보다 훨씬 광범위하게 영향을 끼치며 기술의 발전을 이끌어간다. 기술융합이 생명공학이나 정보기술과 같은 지식집약적 기술 분야에서 두드러지게 나타나고 있으나 데이터 부족 등으로 이에 대한 연구가 제한적일 수밖에 없다. 본 연구에서는 미국 특허 정보를 이용하여 나노바이오 융합기술의 특성을 분석하기 위해 기술의 발전 현황을 관측할 수 있는 여섯 가지 특허 지표를 제시하였다. 그리고 특허의 인용 및 피인용 정보를 활용하여 기술 지식의 융합 및 확산 수준, 기술의 영향력, 잠재력, 축척 정도에 관한 탐색 결과를 얻었다. 그 결과 활발한 특허활동과 큰 잠재적 기술 규모를 보여준 미국특허분류 435(molecular biology and micro bilology), 436(analytical and immunological testing), 424(drug, bio-affecting and body treating composition) 기술 분야는 기술의 확산정도를 측정한 기술확산 지수에서도 높은 값을 보여주어 다양한 기술 분야로의 파급효과 또한 높은 기술임을 보여주었다. 또한 미국특허분류 528(synthetic resins or natural rubbers), 600(surgery), 436(analytical and immunological testing)은 선행기술의 융합수준을 계측하는 기술융합지수에서 높은 값을 보여 이들 기술이 다양한 기술 분야의 지식이 융합된 나노바이오 융합기술임을 보여주고 있다. 이러한 결과들을 바탕으로 현 나노바이오 융합기술의 기술동향이나 공백기술, 원천 특허기술 파악 등을 도출하고 로드맵 기획의 근간으로 사용할 수 있을 것이다. When innovation occurs with the collaboration among different technological/ industrial fields, it is called technology fusion. This phenomena influences broader areas and leads to the development of technologies. Technology fusion appears more frequently in knowledge-intensive fields. Despite its attractiveness, there is limited research in technology fusion due to insufficient data in this field. In this paper, we, utilizing nanobiotechnology, suggest six patent indexes that measure the degree of technological development to analyze the characteristics of fusion technology. By using backward and forward citation information of US patent, we obtain degree of technological diffusion and fusion, technology impact, technology potential, technology cumulativeness, and technology potential. The fields of USPC 435(molecular biology and microbiology), 436(analytical and immunological testing), and 424(drug, bio-affecting and body treating composition) that show the high level of patent activities and technology potential also exhibit the high value of technological diffusion index. This suggests that these technology fields have higher diffusion effects into diverse fields of technologies as well. The values of technology fusion index for 528(synthetic resins or natural rubbers), 600(surgery), and 436(analytical and immunological testing) are high, which implies that these technology fields are developed with diverse fields of technologies. These outcomes can help us to predict the technological trend, new technology opportunities, and the source technologies of nanobiotechnology as well as serve as a basis for technology roadmapping.

Extracellular synthesis of silver nanoparticle by Pseudomonas hibiscicola - Mechanistic approach

Punjabi, Kapil,Mehta, Shraddha,Yedurkar, Snehal,Jain, Rajesh,Mukherjee, Sandeepan,Kale, Avinash,Deshpande, Sunita Techno-Press 2018 Advances in nano research Vol.6 No.1

Biosynthesis of nanoparticles has acquired particular attention due to its economic feasibility, low toxicity and simplicity of the process. Extracellular synthesis of nanoparticles by bacteria and fungi has been stated to be brought about by enzymes and other reducing agents that may be secreted in the culture medium. The present study was carried out to determine the underlying mechanisms of extracellular silver nanoparticle synthesis by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Synthesized nanoparticles were characterized by spectroscopy and electron microscopic techniques. Protein profiling studies were done using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (1D-SDS PAGE) and subjected to identification by Mass Spectrometry. Characterization studies revealed synthesis of 50 nm nanoparticles of well-defined morphology. Total protein content and SDS PAGE analysis revealed a reduction of total protein content in test (nanoparticles solution) samples when compared to controls (broth supernatant). 45.45% of the proteins involved in the process of nanoparticle synthesis were identified to be oxidoreductases and are thought to be involved in either reduction of metal ions or capping of synthesized nanoparticles.

인공혈액의 개발에 관한 연구 진전과 전망

성용길,안세정 한국생체재료학회 2012 생체재료학회지 Vol.16 No.4

For the solution of necessary artificial blood and its substitutes to human being, recent advances on the development of artificial blood have been reviewed from the analysis of literatures published during 26 years from 1986 to 2011. The trends of investigative development for artificial blood, the statistical analysis of the papers published on blood substitutes, and the national distribution of the papers published on artificial blood have been also summarized and discussed precisely. The approach for the application of the stem cell technology to produce the artificial blood has been suggested on the basis of the experienced knowledges of several individual institutions on the world. The perspectives of new methods to produce the artificial blood and its substitutes are bring to be expected and pointed out from the stem cell nanobiotechnology in near future. Key words: artificial blood, blood substitutes, stem cell,