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- 주제어 : Surface sandwich assay (검색결과 4 건)
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Kim, Suhee,Lee, Sanghyuk,Lee, Hye Jin Elsevier 2018 Sensors and actuators. B Chemical Vol.273 No.-
<P><B>Abstract</B></P> <P>A Gold nanorod (NR) enhanced surface sandwich assay utilizing a novel pair of aptamers for the attomolar concentration of norovirus (NoV) capsid protein was developed in conjunction with surface plasmon resonance (SPR). A total of four different DNA aptamer sequences (aptamer I-IV) known to be specific for the NoV protein were examined using SPR for individual binding strength with the NoV protein and for the formation of surface sandwich with the NoV protein, meaning that the chosen aptamer pair possesses different binding epitope towards the NoV protein. One of the aptamer (aptamer II) sequences with the strongest binding constant was covalently tethered onto a chemically modified thin gold chip surface, while the other aptamer I was used for tethering onto the Au NR surface. The surface sandwich complex was formed via the sequential adsorption of NoV capsid protein and Au NR coated aptamer I onto the aptamer II surface. As low as a 70 aM concentration of the NoV protein in buffer solution could be detected, which is 10<SUP>5</SUP> times better than that of using the aptamer-aptamer sandwich platform without any gold NR particles. As a demonstration, the aptamer-NR coated aptamer sandwich assay was applied to analyze NoV capsid protein concentrations spiked in human serum solutions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly sensitive and selective surface sandwich bioassays for norovirus (NoV) capsid protein. </LI> <LI> A pair of DNA aptamer bioreceptors for enhancing the selectivity of NoV capsid protein sensing. </LI> <LI> Gold nanorod-DNA aptamer conjugates to improve the sensitivity for NoV capsid protein sensing. </LI> <LI> Direct analysis of NoV capsid protein concentrations in undiluted human serum samples. </LI> <LI> A lowest detectable concentration of 50 aM NoV capsid protein. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Diba, Farhana Sharmin,Kim, Suhee,Lee, Hye Jin Elsevier 2017 CATALYSIS TODAY - Vol.295 No.-
<P><B>Abstract</B></P> <P>An electrochemical immunosensor involving the formation of a surface sandwich complex on a gold nanoparticle (NP) modified screen printed carbon electrode (SPCE) is demonstrated for the femtomolar detection of amyloid-beta 1–42 peptide (Aβ) in both serum and plasma. Both bioreceptors forming the assay are highly selective antibodies for Aβ, namely antiAβ (12F4) and (1E11) which possess different binding sites for the Aβ peptide. In order to improve the sensing performance for complex biological fluidic matrix analysis, different mixed monolayers of thiol modified polyethylene glycol (PEG) and mercaptopropionic acid (MPA) were self-assembled onto the Au NP-SPCE followed by tethering antiAβ (12F4) to MPA using a heterobifunctional cross linker. Surface sandwich complexes of antiAβ (12F4)/Aβ/antiAβ (1E11)-ALP were then formed via sequential adsorption with the latter antiAβ (1E11) conjugated to alkaline phosphatase (ALP) enzyme. The reaction of surface immobilized ALP with the substrate, 4-amino phenyl phosphate (APP), generated voltammetric detection signals that linearly increased as a function of Aβ concentration. Differential pulse voltammetry was applied to establish a lowest detectable concentration of 100 fM of Aβ with a linear response range from 100 fM to 25 pM. Following optimization, the immunoassay platform was applied in diluted human serum and plasma samples to determine the native concentration of Aβ and the results were validated using a commercially available ELISA test.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A highly sensitive and sensitive sandwich assay for amyloid-beta 1–42 peptide (Aβ). </LI> <LI> Formation of antiAβ (12F4)/Aβ/antiAβ (1E11)-ALP surface sandwich complex. </LI> <LI> Use of a mixed monolayer for sensors surface to improve non-specific adsorption. </LI> <LI> Direct analysis of Aβ in diluted human serum and plasma samples. </LI> <LI> A lowest detectable concentration of 100 fM Aβ with dynamic range of 0.1–25 pM. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A new electrochemical-based surface sandwich assay constructed on Au nanoparticles deposited on a screen printed carbon electrode was developed for the femtomolar detection of unmodified amyloid-beta 1–42 (Aβ) in serum and plasma samples at native concentrations.</P> <P>[DISPLAY OMISSION]</P>
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근접장-분자반응 간의 중첩을 이용한 표면 플라스몬 공명 센서 감도 평가에 관한 연구
류연수,손태황,김동현,Ryu, Yeonsoo,Son, Taehwang,Kim, Donghyun 한국광학회 2013 한국광학회지 Vol.24 No.2
본 논문에서는 근접장-분자반응 간의 중첩을 이용한 표면 플라스몬 공명 (SPR) 바이오센서의 측정감도 평가방법을 연구하였다. 전달행렬 방법을 사용하여 다양한 형태의 중첩적분으로 정의된 광학자취 값을 계산하였고, 샌드위치 및 역샌드위치 면역글로뷸린 (IgG) 어세이에 대해서 실험적으로 측정된 수치와 비교하였다. 이론 및 실험적인 결과와의 비교를 통하여 접선 방향의 전기장을 사용한 광학자취의 경우 그 연속성으로 말미암아 가장 높은 상관계수를 얻을 수 있었으며 이때 광학자취와 측정감도 사이에 97% 이상의 높은 상관계수가 존재함을 보았다. 이러한 상관관계는 SPR 바이오센서의 측정 감도에 관한 메커니즘을 분명하게 설명하며, 분자 스케일 감도를 가지는 SPR 바이오센서 개발에 기여하게 될 것이다. In this study, we have investigated the correlation of far-field detection sensitivity of surface plasmon resonance (SPR) biosensors with optical signatures associated with the near-field overlap of biomolecules. The results confirm a direct relation between the far-field and near-field parameters, particularly for optical signatures defined in terms of lateral electric field components that are tangential to the interface and thus continuous across the interface. The overall correlation between near-field optical signatures and far-field resonance shift exceeded 97%. The results can be highly useful to evaluate detection sensitivity of SPR biosensors that take advantage of complex structures for localization of surface waves.
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알츠하이머 질병 진단을 위한 혈액 바이오마커 검출용 바이오칩 센서 개발
김수희 ( Suhee Kim ),이상혁 ( Sang Hyuk Lee ),이혜진 ( Hye Jin Lee ) 한국공업화학회 2017 공업화학 Vol.28 No.4
해마다 증가하는 알츠하이머 질병에 걸린 환자 수는 전체 노인 인구의 15%에 다다르고 있다. 인지장애를 유발하는 심각한 알츠하이머 질병을 조기에 진단하는 것은 중요한 일이지만 MRI, PET, 척수액 진단법과 같은 기존 진단법은 고비용뿐만 아니라 장시간의 진단으로 환자에게 부담을 줄 수 있다. 이러한 기존 알츠하이머 질병 진단법의 단점을 극복하기 위하여 소량의 환자 시료(예 : 혈액)만으로 신속하게 알츠하이머 질병을 조기에 진단할 수 있는 다양한 바이오센싱 기술을 개발하는 연구가 지속되고 있다. 본 미니총설에서는 알츠하이머 질병 진단에 유용하게 활용될 수 있는 혈액 바이오마커를 정성 및 정량적으로 검출할 수 있는 바이오칩 기반의 센서 기술과 이를 통한 조기진단 기술에 대해 간략하게 서술하고, 이와 관련한 최신 연구현황과 발전방향에 대해 논의하고자 한다. The number of patients suffering from Alzheimer`s disease is increasing year after year and almost approaching 15% of the total elderly population. Although it is critical to detect the early stage of Alzheimer`s disease, which is a serious illness causing cognitive deficits, various existing diagnosis methods such as MRI, PET and CSF analysis could be the burdens for patients due to their high costs and long time to diagnosis. In order to tackle some of challenging issues for such existing diagnosis methods, extensive efforts have been made on developing fast and convenient biochip sensing methodologies for the diagnosis of Alzheimer`s disease with a droplet of patient biofluids (e.g., blood). In this mini-review, we highlight some of the latest biochip sensing technologies that could qualitatively and quantitatively analyze blood biomarkers used for Alzheimer`s disease diagnostics and discuss briefly related research trends and future aspects.
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