Label-free detection of bacterial RNA using polydiacetylene-based biochip

Park, Moo-Kyung,Kim, Kyung-Woo,Ahn, Dong June,Oh, Min-Kyu Elsevier 2012 Biosensors & Bioelectronics Vol.35 No.1

<P><B>Abstract</B></P> <P>We developed a simple and effective polydiacetylene-based, label-free multiplex DNA chip for the detection of various pathogenic microorganisms. A novel immobilization method of PDA vesicles on glass slides was exploited using α-cyclodextrin (α-CD). The surface topography of the efficiently immobilized PDA vesicles was confirmed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Then, oligonucleotides complementary to rRNAs of three pathogenic bacteria were conjugated to the PDA vesicles. Finally, crude lysate of pathogenic bacteria was applied to the PDA biochip. The pathogenic bacteria were specifically detected by DNA–RNA hybridization in an hour. The new PDA sensor was effective in detecting multiple pathogenic bacteria easily and accurately without rigorous purification, amplification, and labeling of their genetic components.</P> <P><B>Highlights</B></P> <P>▸ We constructed a label-free polydiacetylene-based biochip to detect bacterial rRNA. ▸ The polydiacetylene vesicles were immobilized on the slide using α-cyclodextrin. ▸ Crude bacterial lysates were applied to DNA probe-linked polydiacetylene chip. ▸ Multiplex detection of pathogenic bacterial rRNA was demonstrated in an hour.</P>

Polydiacetylene을 이용한 체온 측정 물질의 제조

김희선 ( Huiseon Kim ),허은진 ( Eunjin Heo ),신민재 ( Min Jae Shin ) 한국공업화학회 2021 공업화학 Vol.32 No.2

Considering the current COVID 19 pandemic, herein, we developed a material that can be used to fabricate a device for checking the body temperature of a person who has been exposed to influenza or corona virus. This material was formed by mixing pluronic F127 (F127) with a polydiacetylene (PDA) vesicle, which was formed with 10,12-pentacosadiynoic acid. The color of the system started to change from blue to light purple at 37 ℃, finally turning reddish at 40 ℃. Thus, the developed material can be used to detect changes in body temperature, and thus, detect signs of fever. The mixing ratio of the PDA vesicle and F127 was an important factor for controlling the temperature at which the color change started. The results showed that the color change accompanied by the separation of the PDA vesicle with F127. We believe that this phenomenon plays an important role in reducing the conjugation length in the double and triple bond of PDA.

Polydiacetylene-embedded microbeads for colorimetric and volumetric sensing of hydrocarbons.

Hong, Jaesung,Park, Dong-Hoon,Baek, Sunghoon,Song, Simon,Lee, Chan Woo,Kim, Jong-Man American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.15

<P>Rational design of a hydrocarbon sensor that enables visual differentiation of saturated aliphatic hydrocarbons (SAHCs) is very difficult owing to the lack of useful functional groups that can interact with the sensor system. Here, we report a microbead embedded with polydiacetylene that undergoes faster swelling and faster blue-to-red color change in response to the hydrocarbons of shorter alkyl chains. Accordingly, visual differentiation among n-pentane, n-heptane, n-nonane, and n-undecane was readily achieved. By taking advantage of the collective effect, construction of a sensor system with amplified response was possible. Combination of microfluidic technology (for bead preparation), PDMS (swellable polymeric matrix), and polydiacetylene (colorimetric material) was key to enabling this unique hydrocarbon sensor.</P>

Polydiacetylene이 함유된 PU 나노섬유와 PU 필름의 세균감지 반응속도 비교

김명옥,이정순 한국감성과학회 2019 춘계학술대회 Vol.2019 No.-

In this study, PU nanofibers were prepared by varying the content of PDA using polydiacetylene (PDA), which has colorimetric properties, and the rate of bacterial sensing reaction of the prepared nanofibers was evaluated. In order to compare the detection speed of bacteria in nanofibers, PDA containing films under the same conditions were prepared and compared. From the results, it can be concluded that PDA/PU nanofibers effectively detect gram positive and negative bacteria through color change and have a significantly higher reaction rate than PDA/PU films.

Polydiacetylene immobilized surface-enhanced Raman scattering-encoded beads for label-free detection

전봉현 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

An understanding of protein-protein interactions can be obtained from multiplex protein detection methods, and spectrally coded beads can provide efficient means for this type of detection. In this study, we report that polydiacetylene- coated surface-enhanced Raman scattering- encoded beads (PDA-SERS beads) which can provide an encoding capacity owing to their SERS properties as well as for label-free detection from the PDA layer. We also introduced ligands to the PDA-SERS beads which enabled the detection of target proteins by PDA fluorescence. The fluorescence intensities of biotin-conjugated PDA-SERS beads were increased with an increase in the concentration of streptavidin by using PDA-SERS beads immobilized with biotin. We could detect 2 x 10-8 M of streptavidin by measuring the fluorescence intensity without an additional labeling step.

Patterned polydiacetylene-embedded polystyrene nanofibers based on electrohydrodynamic jet printing

송치호,안희준 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

Electrohydrodynamic (EHD) jet printing is a direct-writing technique which ejects ink through a fine nozzle using an electric field, which has the advantages of high-resolution, rapid printing speed and a wide range of ink selectivity. In this article, the EHD jet printing system is utilized to print patterns of polystyrene (PS) nanofibers. The effect of parameters such as ink concentration, working distance, applied voltage, and stage speed on the diameter of the printed nanofibers was investigated. The EHD jet printing technology is further utilized to print various patterns of polydiacetylene (PDA)-embedded PS nanofibers. The EHD jet printing based nanofiber printing is advantageous over conventional electrospining based approaches in terms of patterned PDA images. In addition, an advanced EHD jet printing system which is adopted for aligned nanofiber printing will expand the application of nanofibers from bio and chemical sensors to tissue engineering and electronics.

Chromatic detection of Cs ions using polydiacetylene-based vesicles containing crown-ether-like ethylene glycol units

Gwon, Young Jin,Kim, Choongho,Lee, Taek Seung Elsevier 2019 Sensors and actuators. B Chemical Vol.281 No.-

<P><B>Abstract</B></P> <P>Radioactive <SUP>137</SUP>Cs, which have a half-life of about 30.17 years, are a major source of nuclear pollution and should be detected and removed. For this purpose, a colorimetric, polydiacetylene (PDA) vesicle-based sensor was synthesized to detect Cs ions. The polymer vesicles consisted of commonly used 10,12-pentacosadiynoic acid (PCDA) and its derivatives. One of the derivatives that contained tetraethylene glycol (TEG) units at the end of the PCDA side chain, and thus it acted as a chelating agent that surrounded the Cs ion like a crown ether. As a result, the main chain of the PDA was distorted by the chelation of TEG and Cs ions, resulting in a blue-red color change. To provide selectivity to the PDA vesicles, another derivative was incorporated to the PDA vesicles with controlled amount, which was not responsive to alkali metal ions. The changes in the colorimetric response of the vesicles were not observed in the presence of other alkali metal ions such as Na, K, and Rb ions, indicating that the TEG units fitted well in size with the ionic radius of the Cs ion, resulting in high selectivity. Solid-state PDA film was also fabricated to provide selective detection of Cs ions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Colorimetric detection of Cs ions using polydiacetylene-based vesicles is proposed. </LI> <LI> Various lengths of EG groups were synthesized at the end of PDA to provide selective Cs ion responsivity. </LI> <LI> The colorimetric response value was used to quantify the degree of color change of PCDA upon exposure to Cs ions. </LI> </UL> </P>

Unique Effects of Cyclodextrins on the Formation and Colorimetric Transition of Polydiacetylene Vesicles

Kim, Jong-Man,Lee, Joo-Seob,Lee, Ji-Seok,Woo, Sang-Yoon,Ahn, Dong June WILEY-VCH Verlag 2005 Macromolecular Chemistry and Physics Vol.206 No.22

<P>Summary: The effects of cyclodextrins (CDs) on the formation and color changes of polymerized 10,12-pentacosadiynoic acid (PCDA) vesicles have been investigated. The strategy used in this study focuses on three different stages in which the CDs can play a role. Among the cyclodextrins investigated, α-CD had most prominent effect on the perturbation of the ordered structures of the PCDA vesicles. Solid precipitates, formed by the PCDA vesicles in the presence of α-CD were shown to be PCDA - α-CD inclusion complexes by using DSC, FTIR and <SUP>1</SUP>H NMR analysis. The results of studies using 4-nitrophenol as an inhibitor and the consideration of inner and outer diameters of cyclodextrins have led to the conclusion that the color induced by the cyclodextrins is due to the formation of inclusion complexes with the polydiacetylene.</P><P> <img src='wiley_img/10221352-2005-206-22-MACP200500331-gra001.gif' alt='wiley_img/10221352-2005-206-22-MACP200500331-gra001'> Graphic Photograph of the polydiacetylene liposomes after 1 h in the presence of 10 mM of the three different cyclodextrins in a HEPES buffer solution. </P>

The fabrication of aminoquinoline-functionalized polydiacetylene-based hybrid vesicles with partially reversible thermochromic property

Yue Gao,Zhiguo Zhao,Xiaojie Huang,Huan Xing,Guang Yang 한국고분자학회 2024 Macromolecular Research Vol.32 No.3

The development of smart materials with reversible thermochromic properties is of great importance in the fields of sensors, displays and anticounterfeitings. In this article, a diacetylene monomer (AQDA) containing aminoquinoline as a head group was synthesized. Together with fluorescein-substituted diacetylene (FDA) and 10,12-pentacosadiynoic acid (PCDA), hybrid PDA vesicles were obtained through self-assembly and topochemical polymerization process. The hybrid PDA vesicles (FDA, AQDA and PCDA with molar ratio of 7:2:1) display an partially reversible green-to-orange red colorimetric change in a wider temperature range from 20 to 100 °C focusing on the stronger hydrogen bonding and aromatic interactions. This work may provide fundamental understanding of the mechanism and strategies for the design of reversible thermochromic PDA materials.

Controlling self-assembling and color-transition of polydiacetylene/zinc(II) ion/zinc oxide nanocomposites by varying pH: Effects of surface charge and head group dissociation

Supakorn Seetha,Rungarune Saymung,Rakchart Traiphol,Nisanart Traiphol 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-

Polydiacetylene/zinc(II) ion/zinc oxide (PDA/Zn2+/ZnO) nanocomposite exhibits reversible thermochromismand dual colorimetric response to acid/base. This contribution presents our ongoingdevelopment of the PDA/Zn2+/ZnO nanocomposite for sensing applications by controlling ZnO surfacecharge and dissociation of PDA headgroup via pH adjustment. At pH >10, negative ZnO surface charge andPDA carboxylate headgroup significantly enhance molecular organization during the self-assemblingprocess. An increase of the nanocomposite amount after photopolymerization is observed. Oppositely,pH <6 results in irreversible-thermochromic nanocomposites. Additionally, the nanocompositesprepared at different pH change color at different concentrations of chemical stimuli. Molecularpacking, local interactions and PDA conformation are investigated.