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- 주제어 : Gut bacterial community (검색결과 4 건)
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안재형,홍인표,복증임,김병용,송재경,원항연 한국미생물학회 2012 The journal of microbiology Vol.50 No.5
The bacterial communities in the guts of the adults and larvae of the Asian honey bee Apis cerana and the European honey bee Apis mellifera were surveyed by pyrosequencing the 16S rRNA genes. Most of the gut bacterial 16S rRNA gene sequences were highly similar to the known honey bee-specific ones and affiliated with Pasteurellaceae or lactic acid bacteria (LAB). The numbers of operational taxonomic units (OTUs, defined at 97% similarity) were lower in the larval guts (6 or 9) than in the adult guts (18 or 20), and the frequencies of Pasteurellaceae-related OTUs were higher in the larval guts while those of LAB-related OTUs in the adult guts. The frequencies of Lactococcus, Bartonella, Spiroplasma,Enterobacteriaceae, and Flavobacteriaceae-related OTUs were much higher in A. cerana guts while Bifidobacterium and Lachnospiraceae-related OTUs were more abundant in A. mellfera guts. The bacterial community structures in the midguts and hindguts of the adult honey bees were not different for A. cerana, but significantly different for A. mellifera. The above results substantiated the previous observation that honey bee guts are dominated by several specific bacterial groups, and also showed that the relative abundances of OTUs could be markedly changed depending on the developmental stage, the location within the gut, and the honey bee species. The possibility of using the gut bacterial community as an indicator of honey bee health was discussed.
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M.T. Ranjith,ManiChellappan,E.R. Harish,D. Girija,P.A. Nazeemc 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), a polyphagous agricultural pest of global importance, harbour diverse bacterial communities in its gut. We analysed the composition and diversity of gut bacteria using Illumina Next-Generation Sequencing of 16S ribosomal RNA amplicons. The data set consisted of 864,813 high-quality paired end sequences with mean length of 150 base pairs. Highly diverse bacterial communities were present in the sample containing approximately 2303 operational taxonomic units (OTUs). A total of 17 bacterial phyla, 34 classes, 84 orders, 173 families, 334 genera, and 707 species were identified from the sequence analysis. Actinobacteria was the most dominant groups, followed by Proteobacteria and Firmicutes. The search on function of different gut inhabiting bacteria of H. armigera revealed their role in nutrition, detoxification of lethal insecticide molecules, and defensive action against pathogens. Insecticidal toxin producing bacterial species were also found associated with the H. armigera gut.
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( Vijayakumar M. Malathi ),( Ravi P. More ),( Rangasamy Anandham ),( Gandhi R. Gracy ),( Muthugounder Mohan ),( Thiruvengadam Venkatesan ),( Sandipan Samaddar ),( Sushil K. Jalali ),( Tongmin Sa ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6
Knowledge about the gut bacterial communities associated with insects is essential to understand their roles in the physiology of the host. In the present study, the gut bacterial communities of a laboratory-reared insecticide-susceptible (IS), and a field-collected insecticide-resistant (IR) population of a major rice pest, the brown planthopper Nilaparvata lugens, were evaluated. The deep-sequencing analysis of the V3 hypervariable region of the 16S rRNA gene was performed using Illumina and the sequence data were processed using QIIME. The toxicological bioassays showed that compared with the IS population, IR population exhibited 7.9-, 6.7-, 14.8-, and 18.7-fold resistance to acephate, imidacloprid, thiamethoxam, and buprofezin, respectively. The analysis of the alpha diversity indicated a higher bacterial diversity and richness associated with the IR population. The dominant phylum in the IS population was Proteobacteria (99.86%), whereas the IR population consisted of Firmicutes (46.06%), followed by Bacteroidetes (30.8%) and Proteobacteria (15.49%). Morganella, Weissella, and Enterococcus were among the genera shared between the two populations and might form the core bacteria associated with N. lugens. The taxonomic-tophenotypic mapping revealed the presence of ammonia oxidizers, nitrogen fixers, sulfur oxidizers and reducers, xylan degraders, and aromatic hydrocarbon degraders in the metagenome of N. lugens. Interestingly, the IR population was found to be enriched with bacteria involved in detoxification functions. The results obtained in this study provide a basis for future studies elucidating the roles of the gut bacteria in the insecticide resistance-associated symbiotic relationship and on the design of novel strategies for the management of N. lugens.
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Chen Chen,Zhang Jingtong,Tan Hao,Fu Zhanyu,Wang Xingya 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1
The gut microbiota is critical for energy and nutrient utilization and plays a role in host immunity in response to environmental changes. The beet armyworm Spodoptera exigua is a worldwide polyphagous agricultural pest and has frequently experienced potentially stressful temperature fluctuations under natural environmental condi tions. However, little is known about the effects of thermal stress on the gut microbiome of this moth pest. Therefore, we investigated the gut microbiome variations, composition and community structure of S. exigua among low-temperature (10 ◦ C), control (26 ◦ C) and high temperature (35 ◦ C) treatments using 16S amplicon sequencing. Overall, 1,192,707 high-quality reads and 762 operational taxonomic units (OTUs) were detected from 15 samples. A total of 289 genera belonging to 19 bacterial phyla were captured, with Firmicutes and Proteobacteria being the most prominent phyla. Alpha diversity metrics indicated no significant differences in the gut bacterial diversity of S. exigua among the three temperature treatments. Principal coordinates and hi erarchical cluster analysis revealed significant differences in the structure of gut microbiota between the lowtemperature treatment and the other two temperature treatments. In addition, PICRUSt2 analysis demon strated that the predicted metagenomes associated with the gut microbiome were amino carbohydrate transport and metabolism, acid transport and metabolism, inorganic ion transport and metabolism and cellular processes. Our study showed that thermal stress induced changes in the gut microbiome of the beet armyworm, which may contribute to better understanding the ecological adaptation of S. exigua under changing temperature trends and to evaluating the use of gut microorganisms as biocontrol agents for this pest.
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