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RISS 인기검색어
- 검색키워드
- 주제어 : EqERV (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Identification and Expression Analyses of Equine Endogenous Retroviruses in Horses
Gim, Jeong-An,Kim, Heui-Soo Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.10
Endogenous retroviruses (ERVs) have been integrated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0.2%/Myr indicated that EqERV-S3 (75.4 million years ago; mya) on chromosome 10 is an old EqERV family and EqERV-P5 (1.2 Mya) on chromosome 12 is a young member. During the evolutionary diversification of horses, the EqERV-I family diverged 1.7 Mya to 38.7 Mya. Reverse transcription quantitative real-time PCR (RT-qPCR) amplification of EqERV pol genes showed greater expression in the cerebellum of the Jeju horse than the Thoroughbred horse. These results could contribute further dynamic studies for horse genome in relation to EqERV gene function.
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Identification and Expression Analyses of Equine Endogenous Retroviruses in Horses
김정안,김희수 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.10
Endogenous retroviruses (ERVs) have been inte-grated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0.2%/Myr indicated that EqERV-S3 (75.4 million years ago; mya) on chromosome 10 is an old EqERV family and EqERV-P5 (1.2 Mya) on chro-mosome 12 is a young member. During the evolutionary diversification of horses, the EqERV-I family diverged 1.7 Mya to 38.7 Mya. Reverse transcription quantitative real-time PCR (RT-qPCR) amplification of EqERV pol genes showed greater expression in the cerebellum of the Jeju horse than the Thoroughbred horse. These results could contribute further dynamic studies for horse genome in relation to EqERV gene function.
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Jo, Ara,Lee, Hee-Eun,Kim, Heui-Soo Elsevier 2019 Gene Vol.687 No.-
<P><B>Abstract</B></P> <P>Horses (<I>Equus caballus</I>) have been domesticated and bred to enhance speed, strength, and agility. Members of the <I>Equus caballus</I> Endogenous Retrovirus (EqERV) family affect several of these abilities in horses. EqERV elements have been integrated in the horse genome during evolution and generate repeat elements such as long terminal repeats (LTRs). LTR sequences are involved in retrovirus replication and play an essential function in post-transcriptional control mechanisms, such as by providing binding sites for microRNAs (miRNAs) or generating miRNA precursors. In this study, we identified a novel miRNA derived from EqERV-E1 LTR using various bioinformatics tools. To examine the relationship between EqERV-E1 LTR and similar elements, we used BLAST2seq and phylogenetic analysis. LTR sequences were located in the untranslated region (UTR) of mRNAs and also formed the stem-loop secondary structure. The sequence was registered in the DDBJ database as LTR derived miRNA under the accession number corresponding to LC383797 (referred to eca-miR-1804). Quantitative polymerase chain reaction (qPCR) to confirm the expression of eca-miR-1804 and the similar miR-1255a, showed an almost identical expression pattern in eight different equine tissues. Therefore, these data imply that the LTR could function as an miRNA, which is expressed in the examined equine tissues. In addition, the current study provides inputs for additional functional studies concerning the LTR of other EqERV families.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel miRNA derived from ERV-E1 LTR in <I>Equus caballus</I> was identified. </LI> <LI> LTR sequences are essential for post-transcriptional mechanisms related to miRNAs. </LI> <LI> LTR sequences were located in horse mRNA UTRs and formed stem-loop structures. </LI> <LI> Eca-miR-1804 and miR-1255a had similar expression patterns in eight equine tissues. </LI> <LI> LTR could function as miRNA and provide information about the biological functions of horse-specific miRNAs. </LI> </UL> </P>
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