Pentacyclic triterpenes from the stem bark of <i>Combretum hartmannianum</i> Schweinf

Morgan, Abubaker M.A.,Mohamed, Azmey E.,Saophea, Chhon,Park, Sang Un,Kim, Young Ho Elsevier 2018 Biochemical systematics and ecology Vol.77 No.-

<P><B>Abstract</B></P> <P>Chemical investigation of the stem bark of <I>Combretum hartmannianum</I> Schweinf (Combretaceae) led to the isolation and identification of nine compounds with pentacyclic triterpene skeletons. These were identified as three ursane-type triterpenes, ursolic acid (<B>1</B>), pomolic acid (<B>2</B>), and corosolic acid (<B>3</B>); one oleanane-type triterpene, arjunic acid (<B>4</B>); and five oleanane-type triterpene glucosides, arjunglucoside I (<B>5</B>), trachelosperoside E-1 (<B>6</B>), combreglucoside (<B>7</B>), chebuloside II (<B>8</B>), and 2α,3β,6β,19α-tetrahydroxyoleanolic acid 28-<I>O</I>-β-<SMALL>D</SMALL>-glucopyranoside (<B>9</B>). The chemical structures of these compounds were elucidated from spectroscopic data and by comparison of these data with previously published results. This is the first report of triterpene compounds from a species of <I>C. hartmannianum</I> and the first report of compounds <B>2</B> and <B>9</B> from a member of the Combretaceae family.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study is the first comprehensive chemical investigation of <I>Combretum hartmannianum</I> Schweinf. </LI> <LI> Nine compounds were reported first herein as isolates from <I>C. hartmannianum</I>, and compounds 2 and 9 are reported for the first time from Combretaceae family. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hydrolyzable tannins from the fruits of <i>Terminalia chebula</i> Retz and their α-glucosidase inhibitory activities

Lee, Dong Young,Kim, Hyun Woo,Yang, Heejung,Sung, Sang Hyun Elsevier 2017 Phytochemistry Vol.137 No.-

<P><B>Abstract</B></P> <P>Nine hydrolyzable tannins, including three previously unknown and six artifacts, were isolated, together with thirty-nine known ones, from the fruits of <I>Terminalia chebula</I> Retz. (Combretaceae). They were identified as 1,2,3-tri-<I>O</I>-galloyl-6-<I>O</I>-cinnamoyl-<I>β</I>-<SMALL>D</SMALL>-glucose, 1,2,3,6-tetra-<I>O</I>-galloyl-4-<I>O</I>-cinnamoyl-<I>β</I>-<SMALL>D</SMALL>-glucose, 4-<I>O</I>-(2″,4″-di-<I>O</I>-galloyl-<I>α</I>-<SMALL>L</SMALL>-rhamnosyl)ellagic acid, 1′-<I>O</I>-methyl neochebulanin, dimethyl neochebulinate, 6′-<I>O</I>-methyl neochebulagate, dimethyl neochebulagate, dimethyl 4′-<I>epi</I>-neochebulagate, and methyl chebulagate by the spectroscopic interpretation. After evaluation for <I>α</I>-glucosidase inhibition of all isolated compounds, 1,2,3,6-tetra-<I>O</I>-galloyl-4-<I>O</I>-cinnamoyl-<I>β</I>-<SMALL>D</SMALL>-glucose and 4-<I>O</I>-(2″,4″-di-<I>O</I>-galloyl-<I>α</I>-<SMALL>L</SMALL>-rhamnosyl)ellagic acid showed significant inhibitory activities with IC<SUB>50</SUB> values of 2.9 and 6.4 μM, respectively. In addition, inhibition kinetic studies showed that both compounds have mixed-type inhibitory activities with the inhibition constants (Ki) of 1.9 and 4.0 μM, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Forty-eight hydrolyzable tannins including nine hitherto unknown were isolated from the fruits of <I>Terminalia chebula.</I> </LI> <LI> α-Glucosidase inhibitory activities of the all isolates were evaluated. </LI> <LI> Two at the nine above compounds showed significant inhibitory activities against α-glucosidase (IC<SUB>50</SUB> 2.9 and 6.4 μM). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Forty-eight hydrolyzable tannins, including three previous unknown and six artifacts were isolated from the fruits of <I>Terminalia chebula</I>. All isolates were evaluated for their <I>α</I>-glucosidase inhibitory activities. Of these, 1,2,3,6-tetra-<I>O</I>-galloyl-4-<I>O</I>-cinnamoyl-<I>β</I>-<SMALL>D</SMALL>-glucose and 4-<I>O</I>-(2″,4″-di-<I>O</I>-galloyl-<I>α</I>-<SMALL>L</SMALL>-rhamnosyl) ellagic acid shown above had the significant inhibitory activities against <I>α</I>-glucosidase.</P> <P>[DISPLAY OMISSION]</P>

Antimicrobial activity and toxicity of Quisqualis indica

Jahan, Fatima N.,Rahman, Mohammad S.,Hossain, Mahboob,Rashid, Mohammad A. Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1

The plant Quisqualis indica (Compositae) has ehnopharmcological reputation of being used as a healing agent in Bangladesh. In this study, preliminary screenings were conducted to look at the antimicrobial susceptibility and cytotoxicity of the plant extract. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract ranged from 8 - 15 mm, 8 - 18 mm, 12 - 20 mm and 10 - 16 mm, respectively at a concentration of 400 ${\mu}g$/disc. All the extractives were also subjected to brine shrimp lethality bioassay for primary cytotoxicity evaluation. Here, the carbon tetrachloride soluble materials demonstrated the highest cytotoxicity with $LC_{50}$ of 0.826 ${\mu}g$/ml, while n-hexane, chloroform and aqueous soluble partitionates of the methanolic extract revealed the $LC_{50}$ of 1.254, 3.866 and 5.366 ${\mu}g$/ml, respectively. This is the first report of the antimicrobial activity and cytotoxicity Q. indica.

Antimicrobial activity and toxicity of Quisqualis indica

Fatima N Jahan,Mohammad S Rahman,Mahboob Hossain,Mohammad A Rashid 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.1

The plant Quisqualis indica (Compositae) has ehnopharmcological reputation of being used as a healing agent in Bangladesh. In this study, preliminary screenings were conducted to look at the antimicrobial susceptibility and cytotoxicity of the plant extract. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract ranged from 8 - 15 mm, 8 - 18 mm, 12 - 20 mm and 10 - 16 mm, respectively at a concentration of 400 μg/disc. All the extractives were also subjected to brine shrimp lethality bioassay for primary cytotoxicity evaluation. Here, the carbon tetrachloride soluble materials demonstrated the highest cytotoxicity with LC50 of 0.826 μg/ml, while n-hexane, chloroform and aqueous soluble partitionates of the methanolic extract revealed the LC50 of 1.254, 3.866 and 5.366 μg/ml, respectively. This is the first report of the antimicrobial activity and cytotoxicity Q. indica. The plant Quisqualis indica (Compositae) has ehnopharmcological reputation of being used as a healing agent in Bangladesh. In this study, preliminary screenings were conducted to look at the antimicrobial susceptibility and cytotoxicity of the plant extract. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract ranged from 8 - 15 mm, 8 - 18 mm, 12 - 20 mm and 10 - 16 mm, respectively at a concentration of 400 μg/disc. All the extractives were also subjected to brine shrimp lethality bioassay for primary cytotoxicity evaluation. Here, the carbon tetrachloride soluble materials demonstrated the highest cytotoxicity with LC50 of 0.826 μg/ml, while n-hexane, chloroform and aqueous soluble partitionates of the methanolic extract revealed the LC50 of 1.254, 3.866 and 5.366 μg/ml, respectively. This is the first report of the antimicrobial activity and cytotoxicity Q. indica.

In vitro evaluation of the antioxidant and cytotoxic activities of constituents of the mangrove Lumnitzera racemosa Willd.

Nguyen Phuong Thao,Bui Thi Thuy Luyen,Chau Ngoc Diep,Bui Huu Tai,김은지,강희경,이상현,장해동,Nguyen The Cuong,Nguyen Van Thanh,Nguyen Xuan Cuong,Nguyen Hoai Nam,Chau Van Minh,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4

This study performed phytochemical and bioactiveassessments of the mangrove Lumnitzera racemosaWilld. leaves. Bioassay-guided fractionation of the methanolicextracts led to the identification of thirty-six compounds(1–36), their structures were elucidated using detailed NMRspectroscopic and MS analysis. The extracts, fractions, andthe isolated compounds were screened for potential antioxidantand cytotoxic activities. Antioxidant assays wereperformed using peroxyl radical-scavenging and reducingassays, whereas cytotoxicity was measured using MTTassays in HL-60 and Hel-299 cell lines. The methanolicextract, CH2Cl2 and n-BuOH fractions (10.0 lg/mL)exhibited potent antioxidant activity, with Trolox equivalent(TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and27.09 ± 0.37 (lM), respectively. In addition, the isolatedcompounds exerted cytotoxic effects in a dose-dependentmanner; compounds 1 and 14 exhibited the most potentcytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29and 0.60 ± 0.16 lM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured thetime-dependent changes in apoptotic markers including thecondensation and fragmentation of nuclear chromatin, andthe downregulation of p-ERK1/2, p-AKT, and c-Myc levels.