Application of Three-phase Hollow Fiber LPME using an Ionic Liquid as Supported Phase for Preconcentration of Malachite Green from Water Samples with HPLC Detection

Zou, Yanmin,Zhang, Zhen,Shao, Xiaoling,Chen, Yao,Wu, Xiangyang,Yang, Liuqing,Zhu, Jingjing,Zhang, Dongmei Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

A novel three-phase hollow fiber liquid phase microextraction was developed for the determination of malachite green (MG) in environmental waters, which selected [BMIM][$PF_6$] mixed with 1% trioctylphosphine oxide (TOPO) as supported phase. Several parameters (accepter phase pH, sample pH, supported phase membrane, volume of accepter phase, salinity, extraction time) that could affect extraction performance were investigated. Under the optimal extraction conditions, the established approach showed excellent characters as: high enrichment factor (212), wide linear range ($0.20-100{\mu}gL^{-1}$), low detection limit ($0.01{\mu}gL^{-1}$), good reproducibility (RSD, 8.9%, n=5) and satisfactory recovery (84.0-106.2%). The method was applied to detect MG at Yangtze River and pond waters in Zhenjiang, Jiangsu province, and 4 sites among 15 sampling sites were found MG with the concentration of $1.73-11.06{\mu}gL^{-1}$, which confirmed that the proposed environmentally friendly method was simple and effective for monitoring MG in aquatic system.

Study on suppression strategy of jet lag effect in melt electrowriting

Zhongfei Zou,Yu Wang,Zhen Shen,Nan Luo 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9

The melt electrowriting (MEW) has broad applications in regenerative medicine and micro-nano electronics. It is an efficient micro-nano scale additive manufacturing technology; however, the fiber jet lag effect of MEW limits the deposition precision and resolution of fiber shape. In this study, the principle of the jet lag effect is studied to overcome the defect of printed structure distortion and improve the ability to print complex structures. A mathematical model of trailing fiber trajectory is established. The study covers jet lag and liquid rope coiling analysis at different speeds. A strategy is adopted by introducing a buffer zone at the corner of the printing structure. The printing path is subdivided and optimized to suppress the influence of jet lag. The results show that the deposited fibers' corner radius is around 63.81±5.66 μm, which is significantly smaller than that of unoptimized groups. Finally, by utilizing the improved printing paths, the high-precision and complex structures are printed, which demonstrates the feasibility of optimizing the buffer zone for the MEW.

Application of Three-phase Hollow Fiber LPME using an Ionic Liquid as Supported Phase for Preconcentration of Malachite Green from Water Samples with HPLC Detection

Yanmin Zou,Zhen Zhang,Xiaoling Shao,Yao Chen,Xiangyang Wu,Liuqing Yang,Jingjing Zhu,Dongmei Zhang 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

A novel three-phase hollow fiber liquid phase microextraction was developed for the determination of malachite green (MG) in environmental waters, which selected [BMIM][PF6] mixed with 1% trioctylphosphine oxide (TOPO) as supported phase. Several parameters (accepter phase pH, sample pH, supported phase membrane, volume of accepter phase, salinity, extraction time) that could affect extraction performance were investigated. Under the optimal extraction conditions, the established approach showed excellent characters as: high enrichment factor (212), wide linear range (0.20-100 μg L-1), low detection limit (0.01 μg L-1), good reproducibility (RSD, 8.9%, n=5) and satisfactory recovery (84.0-106.2%). The method was applied to detect MG at Yangtze River and pond waters in Zhenjiang, Jiangsu province, and 4 sites among 15 sampling sites were found MG with the concentration of 1.73-11.06 μg L-1, which confirmed that the proposed environmentally friendly method was simple and effective for monitoring MG in aquatic system.

Meteorological driven factors of population growth in brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), in rice paddies

Xiao‐Zhen Li,Yi ZOU,Hai-Yan YANG,Hai-Jun XIAO,Jian-Guo WANG 한국곤충학회 2017 Entomological Research Vol.47 No.5

Growth of brown planthopper (BPH) (Nilaparvata lugens Stål) in rice paddies is mainly driven by meteorological factors under similar management practices. By analyzing field investigation and meteorological data collected from 2008 to 2013 in Nanchang, China, we show that BPH population densities and monthly growth rates (BGR) changed greatly from May to October, and these changes were closely associated with meteorological factors. Stepwise regression and path analysis indicated average speed of winds (AW) in June and lowest temperature (LT) in July were the first factors entering analysis, which interpreted 46.20% and 31.90% of their influences on BGR. While highest temperature (HT) in August and average temperature (AT) in September were the most important factors affecting BGR, but their direct path coefficients were all smaller than their corresponding indirect path coefficients. In October, relative humidity (RH), AW and number of raining days (RD) had significant effects on BGR. According to the sum of each meteorological factor entering stepwise regression analysis sequences, we found AW had the utmost effect on BPH growth, followed by AT and RH, but LT and RD least. The work demonstrate dynamic meteorological factors driving BPH growth and outbreak in rice paddies, which would facilitate the development of durable approaches for forecasting and controlling this destructive rice pest.

Knockdown of SMYD3 by RNA interference inhibits cervical carcinoma cell growth and invasion in vitro

Shu Zhen Wang,Xue Gang Luo,Jing Shen,Jia Ning Zou,Yun Hua Lu,Tao Xi 한국생화학분자생물학회 2008 BMB Reports Vol.41 No.4

Diatomaceous earth/zinc oxide micro-composite assisted antibiotics in fungal therapy

LiuHuifang,Qiao Zhen,Jang Yoon Ok,Kim Myoung Gyu,Zou Qingshuang,Lee Hyo Joo,Koo Bonhan,Kim Sung-Han,윤규식,Kim Hyun-Soo,Shin Yong 나노기술연구협의회 2021 Nano Convergence Vol.8 No.32

As the second wave of COVID-19 hits South Asia, an increasing deadly complication ‘fungal infections (such as Mycosis, Candida and Aspergillus) outbreak’ has been raised concern about the insufficient technologies and medicals for its diagnosis and therapy. Biosilica based nano-therapy can be used for therapeutic efficacy, yet their direct role as antibiotic agent with biocompatibility and stability remains unclear. Here, we report that a diatomaceous earth (DE) framework semiconductor composite conjugated DE and in-house synthesized zinc oxide (DE-ZnO), as an antibiotic agent for the enhancement of antibiotic efficacy and persistence. We found that the DE-ZnO composite had enhanced antibiotic activity against fungi ( A. fumigatus ) and Gram-negative bacteria ( E. coli, S. enterica ). The DE-ZnO composite provides enhancing large surface areas for enhancement of target pathogen binding affinity, as well as produces active ions including reactive oxygen species and metal ion for breaking the cellular network of fungi and Gram-negative bacteria. Additionally, the toxicity of DE-ZnO with 3 time less amount of dosage is 6 times lower than the commercial SiO 2 -ZnO. Finally, a synergistic effect of DE-ZnO and existing antifungal agents (Itraconazole and Amphotericin B) showed a better antifungal activity, which could be reduced the side effects due to the antifungal agents overdose, than a single antibiotic agent use. We envision that this DE-ZnO composite can be used to enhance antibiotic activity and its persistence, with less-toxicity, biocompatibility and high stability against fungi and Gram-negative bacteria which could be a valuable candidate in medical science and industrial engineering.

NPC-IGCT Phase Module Clamp Circuit LRC Parameters Design considering FRD snappy Recovery

Yang Ju Zou,Jia Xi Hu,Zhen Yu Ma,Jian Ping Liu,Run Qing Guo,Zhi Xue Zhang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In this paper, a method for neutral point clamping-integrated gate commutated thyristor (NPCIGCT) phase module clamp circuit parameters design considering FRD snappy recovery is present. Based on the snappy recovery theory, and analysis of snappy recovery factors, the paper has shown decreasing current commutating slope can attenuated snappy recovery affect effectively. Then, the paper has shown that it is reasonable for decreasing di/dt by increasing inductance of NPC-IGCT phase module clamp circuit, based on the circuit working principle. Then, the new clamp circuit parameters design method which combine multi-objective optimization solution mathematical module of the circuit, fast recovery diode (FRD) snappy recovery, devices overvoltage and loss is shown in paper. A design example and its test results have demonstrated both IGCT and FRD characteristics have been guaranteed and ensuring safety and reliability of the NPC - IGCT phase module.

Homobifunctional Imidoester Combined Black Phosphorus Nanosheets Used as Cofactors for Nucleic Acid Extraction

류혜방,Qingshuang Zou,김명규,Zhen Qiao,Dao Thi Thuy Nguyen,구본한,이효주,장윤옥,김준기,신용 한국바이오칩학회 2022 BioChip Journal Vol.16 No.1

Studies on nucleic acid-based diagnostics technology have been widely applied to clinical disease diagnosis. In particular, practical effective techniques for the isolation and purification of nucleic acids from pathogenic cells are crucial to ensure the accuracy of analysis. However, numerous organic reagents from the current commercial nucleic acid extraction kits have varied potential side effects in early and further specific diagnosis. Here, we present an easy-to-operate assay for nucleic acid extraction through an outstanding two-dimensional material—black phosphorus (BP), which has attracted significant interest in the diagnosis and treatment of diseases due to its high charge carrier mobility, strong optical absorption, and excellent bioactivity. With the characterization of BP, we proposed an optimized nucleic acid extraction system. A portablesafe single 808 nm near-infrared (NIR) laser was used for the BP thermal heating system for nucleic acid extraction without any larger thermal instrument. We found that the purity and quantity of nucleic acid extracted in the BP-based system were three times higher than in a commercial kit. We also modified the nanosized BP coated with homobifunctional imidoester (HI) to enhance the biomolecule binding with both biocompatibility and surface-crosslinking abilities. We believe that the BP-HI-laser system could be a good candidate for designing nucleic acid extraction for disease diagnosis systems.

Clinical Study of Tumor Angiogenesis and Perfusion Imaging Using Multi-slice Spiral Computed Tomography for Breast Cancer

Xu, Na,Lei, Zhen,Li, Xiao-Long,Zhang, Jun,Li, Chen,Feng, Guo-Quan,Li, Di-Nuo,Liu, Jing-Yi,Wei, Qiang,Bian, Ting-Ting,Zou, Tian-Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Objectives: To explore the correlation between multi-slice spiral CT (MSCT) perfusion parameters and the expression of vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase-2 (MMP-2) in breast cancer. Methods: Forty five breast cancer patients and 16 patients with benign breast tumor, both confirmed by pathology examination, were enrolled. All underwent MSCT perfusion imaging to obtain perfusion maps and data for parameters including blood flow (BF), blood volume (BV) and permeability surface (PS). Cancer patients did not receive treatment prior to surgery. The expression of VEGF and MMP-2 were examined with both immunohistochemistry and Western blotting. Results: The levels of VEGF and MMP-2 by immunohistochemistry were significantly higher in the breast cancer group (P < 0.01) than the benign tumor group. Relative OD values from Western blotting were also higher in cancer cases (P < 0.05). Similarly, the mean MSCT perfusion parameters (BF, BV, PS) were significantly higher in the breast cancer group (P < 0.01), BF and BV positively correlating with VEGF expression (r = 0.878 and 0.809 respectively, P < 0.01); PS and VEGF and MMP-2 expression were also positively correlated (r= 0.860, 0.786 respectively, P < 0.01). Conclusion: There is a correlation between breast cancer MSCT perfusion parameters and VEGF andMMP-2 expression, which might be useful for detection of breast lesions, qualitative diagnosis of breast cancer, and evaluation of breast cancer treatment.

LCL Resonant Compensation of Movable ICPT Systems with a Multi-load

Jie Hua,Hui-Zhen Wang,Yao Zhao,Ai-Long Zou 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6

Compared to LC resonance, LCL resonance has distinct advantages such as a large resonant capability, low voltage and current stresses of the power device, constant voltage or current output characteristics, and fault-tolerance capability. Thus, LCL resonant compensation is employed for a movable Inductive Contactless Power Transfer (ICPT) system with a multi-load in this paper, which achieves constant current output characteristics. Peculiarly, the primary side adopts a much larger compensation inductor than the primary leakage inductor to lower the reactive power, reduce the input current ripple, generate a large current in the primary side, and realize soft-switching. Furthermore, this paper proposes an approximate resonant point for large inductor-ratio LCL resonant compensation through fundamental wave analysis. In addition, the PWM control strategy is used for this system to achieve constant current output characteristics. Finally, an experimental platform is built, whose secondary E-Type coils can ride and move on a primary rail. Simulations and experiments are conducted to verify the effectiveness and accuracy of both the theory and the design method.