Overexpression of Hiwi Promotes Growth of Human Breast Cancer Cells

Wang, Da-Wei,Wang, Zhao-Hui,Wang, Ling-Ling,Song, Yang,Zhang, Gui-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

The Piwi subfamily comprises two argonaute (Ago) family proteins, which are defined by the presence of PAZ and Piwi domains, with well known roles in RNA silencing. Hiwi, a human Piwi subfamily member, has been shown to play essential roles in stem cell self-renewal and gametogenesis. Recently, accumulating reports have indicated that abnormal hiwi expression is associated with poorer prognosis of multiple types of human cancers, including examples in the breast. However, little is known about details of the oncogenic role of hiwi in breast cancers. In present study, we confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines at both mRNA and protein levels. Thus both RT-qPCR and Western blot data revealed significantly higher hiwi in intratumor than peritumor specimens, overexpression being associated with tumor size, lymph node metastasis and histological grade. Hiwi overexpression was also identified in breast cancer cell lines, MDA-MB-231 and MCF-7, and gain-of-function and loss-of-function strategies were adopted to identify the role of hiwi in the MCF-7 cell growth. Results demonstrated that hiwi expression in MCF-7 cells was significantly up- or down-regulated by the two strategies. We next evaluated the influence of hiwi overexpression or knockdown on the growth of breast cancer cells. Both cell count and colony formation assays confirmed promoting roles of hiwi in MCF-7 cells, which could be inhibited by hiwi specific blockage by siRNAs. In summary, the present study confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines, and provided e vidence of promotion by hiwi of cell growth. The results imply an oncogenic role of hiwi in breast cancers.

Genetic diversity and population structure of the amylolytic yeast Saccharomycopsis fibuligera associated with Baijiu fermentation in China

Wang Ju-Wei,Han Pei-Jie,Han Da-Yong,Zhou Sen,Li Kuan,He Peng-Yu,Zhen Pan,Yu Hui-Xin,Liang Zhen-Rong,Wang Xue-Wei,Bai Feng-Yan 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

The amylolytic yeast Saccharomycopsis fibuligera is a predominant species in starters and the early fermentation stage of Chinese liquor (Baijiu). However, the genetic diversity of the species remains largely unknown. Here we sequenced the genomes of 97 S. fibuligera strains from different Chinese Baijiu companies. The genetic diversity and population structure of the strains were analyzed based on 1,133 orthologous genes and the whole genome single nucleotide polymorphisms (SNPs). Four main lineages were recognized. One lineage contains 60 Chinese strains which are exclusively homozygous with relatively small genome sizes (18.55–18.72 Mb) and low sequence diversity. The strains clustered in the other three lineages are heterozygous with larger genomes (21.85–23.72 Mb) and higher sequence diversity. The genomes of the homozygous strains showed nearly 100% coverage with the genome of the reference strain KPH12 and the sub-genome A of the hybrid strain KJJ81 at the above 98% sequence identity level. The genomes of the heterozygous strains showed nearly 80% coverage with both the sub-genome A and the whole genome of KJJ81, suggesting that the Chinese heterozygous strains are also hybrids with nearly 20% genomes from an unidentified source. Eighty-three genes were found to show significant copy number variation between different lineages. However, remarkable lineage specific variations in glucoamylase and α-amylase activities and growth profiles in different carbon sources and under different environmental conditions were not observed, though strains exhibiting relatively high glucoamylase activity were mainly found from the homozygous lineage.

Transiting Exoplanet Monitoring Project (TEMP). IV. Refined System Parameters, Transit Timing Variations, and Orbital Stability of the Transiting Planetary System HAT-P-25

Wang, Xian-Yu,Wang, Songhu,Hinse, Tobias C.,Li, Kai,Wang, Yong-Hao,Laughlin, Gregory,Liu, Hui-Gen,Zhang, Hui,Wu, Zhen-Yu,Zhou, Xu,Zhou, Ji-Lin,Hu, Shao-Ming,Wu, Dong-Hong,Peng, Xi-Yan,Chen, Yuan-Yuan Astronomical Society of the Pacific 2018 Publications of the Astronomical Society of the Pa Vol.130 No.988

Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine

Wang, Gui-Ping,Hui, Zhen,Li, Feng,Zhao, Mei-Rong,Zhang, Jin,Wang, Wei The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.3

Within their natural habitat, crops are often subjected to drought and heat stress, which suppress crop growth and decrease crop production. Causing overaccumulation of glycinebetaine (GB) has been used to enhance the crop yield under stress. Here, we investigated the response of wheat (Triticum aestivum L.) photosynthesis to drought, heat stress and their combination with a transgenic wheat line (T6) overaccumulating GB and its wild-type (WT) Shi4185. Drought stress (DS) was imposed by controlling irrigation until the relative water content (RWC) of the flag leaves decreased to between 78 and 82%. Heat stress (HS) was applied by exposing wheat plants to $40^{\circ}C$ for 4 h. A combination of drought and heat stress was applied by subjecting the drought-stressed plants to a heat stress as above. The results indicated that all stresses decreased photosynthesis, but the combination of drought and heat stress exacerbated the negative effects on photosynthesis more than exposure to drought or heat stress alone. Drought stress decreased the transpiration rate (Tr), stomatal conductance (Gs) and intercellular $CO_2$ concentration (Ci), while heat stress increased all of these; the deprivation of water was greater under drought stress than heat stress, but heat stress decreased the antioxidant enzyme activity to a greater extent. Overaccumulated GB could alleviate the decrease of photosynthesis caused by all stresses tested. These suggest that GB induces an increase of osmotic adjustments for drought tolerance, while its improvement of the antioxidative defense system including antioxidative enzymes and antioxidants may be more important for heat tolerance.

Integration Sites and Genotype Distributions of Human Papillomavirus in Cervical Intraepithelial Neoplasia

Wang, Li,Dai, Shu-Zhen,Chu, Hui-Jun,Cui, Hong-Fei,Xu, Xiao-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Objectives: To analyse HPV integration prevalence and genotype distributions in cervical intraepithelial neoplasia (CIN) in east part of China, furthermore to assess preferential sites for common HPV integrations and provide baseline information for cervical abnormality screening and prevention. Methods: Integration of HPV in 113 paraffin-embedded cervical intraepithelial neoplasia samples was assessed using Gencap technology in Key Laboratory of Biotechnologies in BGI-Shenzhen. Results: 64 samples were HPV-integrated and as the cervical lesions increased, the integration rate became higher significantly (P=0.002). Fifteen different HPV genotypes were detected, 14 high-risk (16, 18, 31, 33, 51, 52, 56, 58, 66, 68) and 1 low-risk (11). The most common genotypes were HPV-16, 58, 33, 52, 66, and 56. Thirteen patients had co-integration involving mainly HPV-16 and 58. The frequency of HPV gene disruption was higher in L1 and E1 genes than in other regions of the viral genomes. Conclusion: Some 56.6% of CIN lesions in Qingdao had HPV integrations, and 67.2% of HPV-integrated patients were HPV-16 and 58, more prone to be integrated in younger patients below 45 years old. There exist preferential sites for HPV-16 and HPV-58 integration, and they are more likely to be disrupted in the L1 and E1 loci.

Human Papillomavirus Vaccine Awareness, Acceptability, and Decision-Making Factors among Chinese College Students

Wang, Shao-Ming,Zhang, Shao-Kai,Pan, Xiong-Fei,Ren, Ze-Fang,Yang, Chun-Xia,Wang, Zeng-Zhen,Gao, Xiao-Hong,Li, Man,Zheng, Quan-Qing,Ma, Wei,Zhao, Fang-Hui,Qiao, You-Lin,Sivasubramaniam, Priya Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: College students are recommended as the target groups for catch-up human papillomavirus (HPV) vaccination. Systematical exploration of awareness, acceptability, and decision-making factors of HPV vaccination among Chinese college students has been limited. Materials and Methods: A multi-center survey was conducted in mainland China between November 2011 and May 2012. College students aged 18-22 years were stratified by their grade, gender, and major for sampling. Socio-demographic and HPV-related information such as knowledge, perceptions, acceptability, and attitudes were collected through a questionnaire. Results: A total of 3,497 undergraduates completed the questionnaire, among which 1,686 were males. The acceptability of the HPV vaccine was high (70.8%). Undergraduates from high-level universities, at lower grade, or with greater prior knowledge of HPV vaccines showed higher acceptability of HPV vaccination ($p_{trend}$ <0.001). Additionally, undergraduates with vaccination experience outside the National Expanded Program on Immunization (OR=1.29; 95%CI: 1.10-1.51) or fear of HPV-related diseases (OR=2.79; 95%CI: 2.28-3.41) were more willing to accept HPV vaccination. General knowledge of HPV vaccine was low among undergraduates, and safety was a major concern (71.05%). The majority of students wished to pay less than 300RMB for HPV vaccine and chose the Chinese Center for Disease Control and Prevention as the most appropriate venue for vaccination. Conclusions: Although most undergraduates demonstrate positive attitudes towards HPV vaccination, challenges pertaining to introduction exist in China. Corresponding proactive education and governmental subsidy to do so are urgently needed by this age-group population. Suggestions and potential strategies indicated may help shape the future HPV vaccination program in China.

Production of diacylglycerols by esterification of oleic acid with glycerol catalyzed by diatomite loaded SO4 2-/TiO2

Zhen Zhang,Hui Hua Huang,Xiang Ma,Guanghui Li,Yong Wang,Guo Sun,Yinglai Teng,Rian Yan,Ning Zhang,AiJun Li 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.53 No.-

A new and efficient technique is described for the production of diacylglycerols (DAGs) by the esterification of oleic acid with glycerol catalyzed by diatomite-loaded SO4 2/TiO2. DAGs show some potential health benefits compared to triacylglycerols, and also can be used to produce the novel industrial plasticizer epoxy fatty acid methyl ester in material science. Diatomite-loaded SO4 2/TiO2 catalyst was prepared and characterized, and the selected conditions for the synthesis of DAGs were determined to be: reaction time = 6.0 h, temperature = 210 C, catalyst loading = 0.1% of the oleic acid weight, and mass ratio of oleic acid to glycerol = 2:1. Under these conditions, DAGs yield reached 59.6% with a purity of 69.6% after a one-stage molecular distillation. Diatomite-loaded SO4 2/TiO2 as a solid catalyst could be recycled and reused with high catalytic efficiency. Under the same conditions, diatomite-loaded SO4 2/TiO2 showed a better catalytic performance than the commercial solid acid SO4 2/ZrO2-Al2O3. Based on this, a two-step reaction method for the production of DAGs was performed and provided a yield similar to the one-step method (58.3% vs. 59.6%), but with a shorter reaction time (4 h vs. 6 h). It is concluded that a two-step reaction method could be a better alternative to the one-step production of DAGs in the presence of diatomite-loaded SO4 2/TiO2.

Adsorption of Lead(II) from Aqueous Solution by Two Modified Porous Chelating Resins Based on (3-Mercaptopropyl) Trimethoxysilane

Zhen-Zheng Wang,Hong-Dong Duan,Xia Meng,Yi-Fan Zhang,Hui-Min Qi 한국고분자학회 2016 Macromolecular Research Vol.24 No.9

Two novel types of thiol-based chelating resins were fabricated by modifying poly(2-hydroxyethyl methacrylate- co-glycerol dimethacrylate) (P(HEMA-GDMA)) and poly(2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) (P(HEMA-EGDMA)) with (3-mercaptopropyl) trimethoxysilane(MPS), and used to adsorb lead(II) from aqueous solution. The effects of initial lead(II) concentrations, solution pH, coexisting ions on adsorption of lead(II) were investigated. After modifying, the product P(HEMA-GDMA)-SH has excellent adsorption capacity (118.56 mg/g) for lead(II) at initial lead(II) concentration of 200 mg/L. The resins could easily be regenerated and were reused 6 times without significant loss of adsorption capacity. Besides, the presence of coexisting ions did not markedly influence the adsorption capacities of the resins. The results indicate that the functional thiol-based resins are efficient adsorbents for the removal of toxic lead(II) ions in water treatment.

Aprepitant in the Prevention of Vomiting Induced by Moderately and Highly Emetogenic Chemotherapy

Wang, Shi-Yong,Yang, Zhen-Jun,Zhang, Zhe,Zhang, Hui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Chemotherapy is a major therapeutic approach for malignant neoplasms; however, due to the most common adverse events of nausea and vomiting, scheduled chemotherapeutic programs may be impeded or even interrupted, which severely impairs the efficacy. Aprepitants, 5-HT3 antagonists and dexamethasone are primary drugs used to prevent chemotherapy-induced nausea and vomiting (CINV). These drugs have excellent efficacy for control of acute vomiting but are relatively ineffective for delayed vomiting. Aprepitant may remedy this deficiency. Substance P was discovered in the 1930s and its association with vomiting was confirmed in the 1950s. This was followed by a period of non-peptide neurokinin-1 (NK-1) receptor antagonist synthesis and investigation in preclinical studies and clinical trials (phases I, II and III). The FDA granted permission for the clinical chemotherapeutic use of aprepitant in 2003. At present, the combined use of aprepitant, 5-HT3 antagonists and dexamethasone satisfactorily controls vomiting but not nausea. Therefore, new therapeutic approaches and drugs are still needed.

0BTimSim: An Efficient Simulator for Wireless Ad-hoc Networks

Hui Xia,Jia Yu,Guo-Dong Wang,Zhen-Kuan Pan 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4

With the growth of increasingly complex in wireless ad-hoc networks, performance modeling and evaluation play a crucial part in their design process to ensure successful deployment and exploitation in practice. Compared with the actual implementation (testbeds), simulation has become a valuable tool, which allows studying large scale systems that cannot be built practically. However, most simulators currently used for such networks are intended to evaluate individual routing protocol, which should be extracted from the source code and rewritten in accordance with the simulator’s requirements. Thus, in this paper, we design a new wireless ad-hoc network simulator, called Time Step-based Wireless Ad-hoc Network Simulator (TimSim). It is intended to facilitate the migration of simulation code to real devices via providing useful APIs with real device driver. We use a discrete event processor with time step-based feature as the simulating engine, and the data can be transmitted in bit-level. A unique feature of TimSim is its ability to support the simulation of multi-thread programming. Additionally, we import database access to store the simulation information. Extensive experiments have been conducted to evaluate the efficiency of TimSim. Furthermore, a case study on a real system has been presented to illustrate its practicability.