High expression of keratin 6C is associated with poor prognosis and accelerates cancer proliferation and migration by modulating epithelial–mesenchymal transition in lung adenocarcinoma

Hai‑Bo Hu,Xiao‑Ping Yang,Pei‑Xia Zhou,Xin‑Ai Yang,Bin Yin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background Lung adenocarcinoma (LUAD) is a more frequent subtype of lung cancer and most cases are discovered in the late stages. The proliferation and metastasis of LUAD are pivotal for disease progression. Despite unremitting deeper understanding of LUAD biology, the mechanisms involved in the proliferation and metastasis of LUAD remain unclear. The objective of our article was to inquiry the expression and the function of keratin 6C (KRT6C) in LUAD cells. Methods First, the expression level and prognostic value of KRT6C in LUAD tissues were analyzed on the basis of the data acquired from TCGA database. Through qRT-PCR, the expression level of KRT6C on LUAD cell lines (A549, H1299, PC-9) and human normal lung cell line MRC-5 was tested. After that, CCK8 and colony formation assays was utilized to detect cell proliferation. In addition, to explore the influence of KRT6C on LUAD migration and invasion ability, scratch wound healing and transwell assays were utilized. Through western blotting, the protein expression levels of KRT6C, PCNA, E-cadherin, N-cadherin, Snail and Vimentin were detected. Results The outcomes revealed that KRT6C was highly expressed in LUAD tissues and cell lines. Besides, elevated level of KRT6C was related to worse prognosis in LUAD patients. Ablation of KRT6C restrained proliferation, migration and invasion of A549 cells. KRT6C deficiency augmented the expression of E-cadherin as well as reduced the expression of N-cadherin, Snail and Vimentin. Conclusion Above all, these consequences indicated that depletion of KRT6C suppressed A549 cell proliferation, migration and invasion, which might be achieved by regulating EMT. In general, KRT6C is identified as a potential therapeutic target for LUAD.

AIDS-Related Non-Hodgkin Lymphoma: Imaging Feature Analysis of 27 Cases and Correlation with Pathologic Findings

Yang, Jun,Wang, Peng,Lv, Zhi-Bin,Wei, Lian-Gui,Xu, Yun-Liang,Zhou, An,Xu, Dong-Hai,Ma, Da-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Some tumor types are related to HIV, including non-Hodgkin lymphoma (NHL). The morbidity and mortality of NHL has remained high, even after highly active antiretroviral therapy (HAART) was introduced. We collected cases of AIDS with NHL, and evaluated the imaging features and strategies for diagnosis. Materials and Methods: There were 27 patients with AIDS and tumors confirmed by pathology. There were 9 patients with Burkitt lymphoma, 16 with diffuse large B cell lymphomas (DLBCLs), and 2 with primary central nervous system (PCNS) lymphomas. All of the patients underwent a series of imaging studies. Three radiologists analyzed the images, and any disagreement was discussed until consensus was reached. Results: The radiologic manifestations of AIDS with NHL were mainly masses and lymphadenopathy, 3 patients having one mass and 12 two or more masses. 7 patients had lymphadenopathy in one site and 3patients had lymphadenopathy in two or more sites. Coarse mucosal folds, thickening of the gastrointestinal wall, and lumen narrowing were typical manifestations of NHL within the gastrointestinal tract. There were 4 patients with masses and 5 with lymphadenopathy inthe 9 with Burkitt lymphoma, and 11 patients with masses 5 with lymphadenopathy in the 16 with DLBCLs. Conclusion: NHL is a malignancy that usually occurs in patients with AIDS. Imaging is an important method by which to evaluate lesions, masses, and lymphadenopathy. Fine needle aspiration biopsy and stereotaxis biopsy are useful methods by which to diagnose NHL.

Effect of L-carnitine on sperm quality during liquid storage of boar semen

Yang Kang,Wang Na,Guo Hai-Tao,Wang Jing-Ran,Sun Huan-Huan,Sun Liang-Zhen,Yue Shun-Li,Zhou Jia-Bo 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.

Preoperative Serum CEA and CA19-9 in Gastric Cancer - a Single Tertiary Hospital Study of 1,075 Cases

Zhou, Yang-Chun,Zhao, Hai-Jian,Shen, Li-Zong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

To evaluate the clinical impact of preoperative serum CEA and CA19-9 on resectable gastric cancer (GC), a total of 1,075 consecutive cases with gastric adenocarcinoma were obtained retrospectively from January 2012 and December 2013 in a single tertiary hospital, and the relationships between serum CEA, CA19-9 and clinicopathologic features were investigated. Positive preoperative serum rates of CEA and CA19-9 were 22.4% and 12.3% respectively, levels significantly correlating with each other and depth of invasion, lymph node involvement, pTNM and stage. The CEA level also presented a remarkable association with lymphovascular invasion. Both CEA and CA19-9 positivity significantly and positively correlated with depth of invasion, nodal involvement, pTNM stage, lymphovascular invasion, tumor size and tumor location. Stratified analyses according to gender or tumor location showed preoperative CEA or CA19-9 had different associations with clinicopathologic features in different gender subgroups or location subgroups. Preoperative serum CA19-9 positivity may be more meaningful for tumor size rather than CEA. In conclusion, preoperative serum CEA and CA19-9 correlate with disease progression of GC, and may have applications in aiding more accurate estimation of tumor stage, decision of treatment choice and prognosis evaluation.

A Functional SNP in the MDM2 Promoter Mediates E2F1 Affinity to Modulate Cyclin D1 Expression in Tumor Cell Proliferation

Yang, Zhen-Hai,Zhou, Chun-Lin,Zhu, Hong,Li, Jiu-Hong,He, Chun-Di Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: The MDM2 oncogene, a negative regulator of p53, has a functional polymorphism in the promoter region (SNP309) that is associated with multiple kinds of cancers including non-melanoma skin cancer. SNP309 has been shown to associate with accelerated tumor formation by increasing the affinity of the transcriptional activator Sp1. It remains unknown whether there are other factors involved in the regulation of MDM2 transcription through a trans-regulatory mechanism. Methods: In this study, SNP309 was verified to be associated with overexpression of MDM2 in tumor cells. Bioinformatics predicts that the T to G substitution at SNP309 generates a stronger E2F1 binding site, which was confirmed by ChIP and luciferase assays. Results: E2F1 knockdown downregulates the expression of MDM2, which confirms that E2F1 is a functional upstream regulator. Furthermore, tumor cells with the GG genotype exhibited a higher proliferation rate than TT, correlating with cyclin D1 expression. E2F1 depletion significantly inhibits the proliferation capacity and downregulates cyclin D1 expression, especially in GG genotype skin fibroblasts. Notably, E2F1 siRNA effects could be rescued by cyclin D1 overexpression. Conclusion: Taken together, a novel modulator E2F1 was identified as regulating MDM2 expression dependent on SNP309 and further mediates cyclin D1 expression and tumor cell proliferation. E2F1 might act as an important factor for SNP309 serving as a rate-limiting event in carcinogenesis.

Multiple Impairments in Male Reproduction 1 (mimr1), a Novel Male-Sterile Mutant of Arabidopsis thaliana, Shows Several Defects in Male Reproductive Development

Hai-Yan Chen,Yue-Feng Guan,Xue-Yong Huang,Yu-Ting Wu,Fen-Fei Wang,Ju-Fang Gao,Que Zhou,Zhong-Nan Yang,Jia-Yao Liu,Hong-Xia Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3

We have characterized a new male-sterile mutant in Arabidopsis that exhibits conditional sterility but has restored fertility when drought-stressed. This mutant,multiple impairments in male reproduction 1 (mimr1),shows pleiotropic defects in both vegetative and reproductive development. Examination with dissecting and scanning electron microscopes revealed that its pollen grains are not effectively released from the anther locule after dehiscence, and anther differentiation is defective. Growth of the style and stamen filaments are also abnormal. Histological analysis demonstrated that these phenomena are due not only to a noticeably reduced extension of the stamen but also greater elongation of the pistil. Genetic analysis indicated that mimr1 is a single locus recessive nuclear mutant. The mutation can be mapped to a locus strongly linked to a 1200-kb region on Chromosome 3. Meta-analysis of expression patterning presented several candidate genes in that region. No mutants with similar phenotypes have previously been reported, suggesting that mimr1 is a novel male-sterile locus. Characterization of MIMR1 will provide further insights into the molecular basis for the development of plant reproductive organs.

The effects of the circulating water tunnel wall and support struts on hydrodynamic coefficients estimation for autonomous underwater vehicles

Huang, Hai,Zhou, Zexing,Li, Hongwei,Zhou, Hao,Xu, Yang The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-

This paper investigates the influence of the Circulating Water Channel (CWC) side wall and support struts on the hydrodynamic coefficient prediction for Autonomous Underwater Vehicles (AUVs) experiments. Computational Fluid Dynamics (CFD) method has been used to model the CWC tests. The hydrodynamic coefficients estimated by CFD are compared with the prediction of experiments to verify the accuracy of simulations. In order to study the effect of side wall on the hydrodynamic characteristics of the AUV in full scale captive model tests, this paper uses the CWC non-dimensional width parameters to quantify the correlation between the CWC width and hydrodynamic coefficients of the chosen model. The result shows that the hydrodynamic coefficients tend to be constant with the CWC width parameters increasing. Moreover, the side wall has a greater effect than the struts.

Lithium chloride’s inhibition of 3T3-L1 cell differentiation by regulating the Wnt/β-catenin pathway and enhancing villin 2 expression

Yu-Yang Chi,Jing-Lin Shen,Jing Zhang,Anshan Shan,Shu-Ling Niu,Chang-Hai Zhou,이홍구,Yong-Cheng Jin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

The aim of this study is to reveal the relation among villin 2, Wnt/β-catenin, andadipogenesis by adding appropriate lithium chloride (LiCl). The study comprises three parts: theselection of LiCl concentration, the effect of LiCl on adipocyte differentiation during and afterdifferentiation induction. By comprehensively analyzing the results of the experiments, we proved thatLiCl can inhibit adipocyte differentiation and enhance villin 2 and β-catenin expressions not only duringdifferentiation induction but also after it. Moreover, villin 2 has a significant impact on β-catenin. Wesuggest that villin 2 may participate in Wnt/β-catenin signaling.

Fluorine-doped porous carbon-decorated Fe₃O₄-FeF₂ composite versus LiNi_0.5Mn_1.5O₄ towards a full battery with robust capability

Ming, Hai,Ming, Jun,Kwak, Won-Jin,Yang, Wenjing,Zhou, Qun,Zheng, Junwei,Sun, Yang-Kook Elsevier 2015 ELECTROCHIMICA ACTA Vol.169 No.-

<P><B>Abstract</B></P> <P>A new fluorine-doped porous carbon-decorated Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB> composite, referred to as Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>, was prepared for the first time. The formation mechanism is discussed, and a new concept of introducing double layers of FeF<SUB>2</SUB> and CF<SUB>x</SUB> into the oxide-based anode is presented for lithium ion batteries. Varying the amount of fluorine precursor, derivatives of Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB>x</SUB> and FeF<SUB>2</SUB>@CF<SUB>x</SUB> were further obtained, allowing an original analysis of their electrochemical behaviors. As-prepared Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> can deliver a high capacity of 718mAhg<SUP>−1</SUP> at 50mAg<SUP>−1</SUP>. Under a hash rate of 1600mAg<SUP>−1</SUP>, the capacity of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> (around 338mAhg<SUP>−1</SUP>) is higher than that (200mAhg<SUP>−1</SUP>) of FeF<SUB>2</SUB>@CF<SUB>x</SUB>. Further, its capacity retention of 97% over 100 cycles is much better than the 59.4% observed for Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB>x</SUB>. The positive effect of the CF<SUB>x</SUB> layer on the electronic conductivity and ionic diffusion ability was confirmed. The role of FeF<SUB>2</SUB> in the stabilization of the structure of CF<SUB>x</SUB> and Fe<SUB>3</SUB>O<SUB>4</SUB> is also discussed. Further, a new battery composed of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with a robust rate capability was assembled and delivered a reversible capacity of 565mAhg<SUP>−1</SUP> (<I>vs.</I> anode) at 100mAg<SUP>−1</SUP> with a high potential of 3.3V and a capacity retention of 81.5% over 50 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new anode of fluorine-doped porous Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> composite is readily prepared. </LI> <LI> The CF<SUB>x</SUB> layer enhances the conductivity of Fe<SUB>3</SUB>O<SUB>4</SUB> and ensures a fast Li<SUP>+</SUP> diffusion. </LI> <LI> The FeF<SUB>2</SUB> can stabilize the structure of Fe<SUB>3</SUB>O<SUB>4</SUB> during the (dis) charge process. </LI> <LI> The Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> can deliver a high capacity with a robust rate capability. </LI> <LI> A full cell of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with high performance is assembled. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A new anode of fluorine doped porous Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> composite with double layers of FeF<SUB>2</SUB> and CF<SUB>x</SUB> was presented for the first time, and a high rate capability was obtained in lithium ion battery. Besides, a new full battery of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with a high capacity of 565mAhg<SUP>−1</SUP> (<I>vs</I>. anode) at the current density of 100mAg<SUP>−1</SUP> was successfully introduced. It demonstrated a robust rate capability, high operating potential of 3.3V and fine cycle ability over 50 cycles with capacity retention of 81.5%.</P> <P>[DISPLAY OMISSION]</P>

Surfactant-Assisted Synthesis of Fe₂O₃ Nanoparticles and F-Doped Carbon Modification toward an Improved Fe₃O₄@CF_<i>x</i>/LiNi_0.5Mn_1.5O₄ Battery

Ming, Hai,Ming, Jun,Oh, Seung-Min,Tian, Shu,Zhou, Qun,Huang, Hui,Sun, Yang-Kook,Zheng, Junwei American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.17

<P>A simple surfactant-assisted reflux method was used in this study for the synthesis of cocklebur-shaped Fe<SUB>2</SUB>O<SUB>3</SUB> nanoparticles (NPs). With this strategy, a series of nanostructured Fe<SUB>2</SUB>O<SUB>3</SUB> NPs with a size distribution ranging from 20 to 120 nm and a tunable surface area were readily controlled by varying reflux temperature and the type of surfactant. Surfactants such as cetyltrimethylammonium bromide (CTAB), polyvinylpyrrolidone (PVP), poly(ethylene glycol)-<I>block</I>-poly(propylene glycol)-<I>block</I>-poly(ethylene glycol) (F127) and sodium dodecyl benzenesulfonate (SDBS) were used to achieve large-scale synthesis of uniform Fe<SUB>2</SUB>O<SUB>3</SUB> NPs with a relatively low cost. A new composite of Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB><I>x</I></SUB> was prepared by coating the primary Fe<SUB>2</SUB>O<SUB>3</SUB> NPs with a layer of F-doped carbon (CF<SUB><I>x</I></SUB>) with a one-step carbonization process. The Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB><I>x</I></SUB> composite was utilized as the anode in a lithium ion battery and exhibited a high reversible capacity of 900 mAh g<SUP>–1</SUP> at a current density of 100 mA g<SUP>–1</SUP> over 100 cycles with 95% capacity retention. In addition, a new Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB><I>x</I></SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> battery with a high energy density of 371 Wh kg<SUP>–1</SUP> (vs cathode) was successfully assembled, and more than 300 cycles were easily completed with 66.8% capacity retention at 100 mA g<SUP>–1</SUP>. Even cycled at the high temperature of 45 °C, this full cell also exhibited a relatively high capacity of 91.6 mAh g<SUP>–1</SUP> (vs cathode) at 100 mA g<SUP>–1</SUP> and retained 54.6% of its reversible capacity over 50 cycles. Introducing CF<SUB><I>x</I></SUB> chemicals to modify metal oxide anodes and/or any other cathode is of great interest for advanced energy storage and conversion devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-17/am504144d/production/images/medium/am-2014-04144d_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am504144d'>ACS Electronic Supporting Info</A></P>