Hsp20 Promotes Endothelial Progenitor Cell Angiogenesis via Activation of PI3K/Akt Signaling Pathway under Hypoxia

Han Zhiqi,He Xuan,Feng Yuan,Jiang Weidong,Zhou Nuo,Huang Xuanping 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: Mandibular distraction osteogenesis (MDO) is a kind of endogenous tissue engineering technology that lengthens the jaw and opens airway so that a patient can breathe safely and comfortably on his or her own. Endothelial progenitor cells (EPCs) are crucial for MDO-related angiogenesis. Moreover, emerging evidence suggests that heat shock protein 20 (Hsp20) modulates angiogenesis under hypoxic conditions. However, the specific role of Hsp20 in EPCs, in the context of MDO, is not yet known. The aim of this study was to explore the expression of Hsp20 during MDO and the effects of Hsp20 on EPCs under hypoxia. METHODS: Mandibular distraction osteogenesis and mandibular bone defect (MBD) canine model were established. The expression of CD34, CD133, HIF-1a, and Hsp20 in callus was detected by immunofluorescence on day 14 after surgery. Canine bone marrow EPCs were cultured, with or without optimal cobalt chloride (CoCl2) concentration. Hypoxic effects, caused by CoCl2, were evaluated by means of the cell cycle, cell apoptosis, transwell cell migration, and tube formation assays. The Hsp20/KDR/PI3K/Akt expression levels were evaluated via immunofluorescence, RT-qPCR, and western blot. Next, EPCs were incorporated with either Hsp20-overexpression or Hsp20-siRNA lentivirus. The resulting effects were evaluated as described above. RESULTS: CD34, CD133, HIF-1a, and Hsp20 were displayed more positive in the callus of MDO compared with MBD. In addition, hypoxic conditions, generated by 0.1 mM CoCl2, in canine EPCs, accelerated cell proliferation, migration, tube formation, and Hsp20 expression. Hsp20 overexpression in EPCs significantly stimulated cell proliferation, migration, and tube formation, whereas Hsp20 inhibition produced the opposite effect. Additionally, the molecular mechanism was partly dependent on the KDR/PI3K/Akt pathway. CONCLUSION: In summary, herein, we present a novel mechanism of Hsp20-mediated regulation of canine EPCs via Akt activation in a hypoxic microenvironment.

Experimental Study of Adaptive Sliding Mode Control for Vibration of a Flexible Rectangular Plate

Jingyu Yang,Zhiqi Liu,Xuanming Cui,Shiying Qu,Chu Wang,Zhou Lanwei,Guoping Chen 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.1

This paper aims to address the intelligent active vibration control problem of a flexible rectangular plate vibration involving parameter variation and external disturbance. An adaptive sliding mode (ASM) MIMO control strategy and smart piezoelectric materials are proposed as a solution, where the controller design can deal with problems of an external disturbance and parametric uncertainty in system. Compared with the current ‘classical’ control design, the proposed ASM MIMO control strategy design has two advantages. First, unlike existing classical control algorithms, where only low intelligence of the vibration control system is achieved, this paper shows that high intelligent of the vibration control system can be realized by the ASM MIMO control strategy and smart piezoelectric materials. Second, the system performance is improved due to two additional terms obtained in the active vibration control system. Detailed design principle and rigorous stability analysis are provided. Finally, experiments and simulations were used to verify the effectiveness of the proposed strategy using a hardware prototype based on NI instruments, a MATLAB/SIMULINK platform, and smart piezoelectric materials.

An FPGA-Based Modified Adaptive PID Controller for DC/DC Buck Converters

Lv, Ling,Chang, Changyuan,Zhou, Zhiqi,Yuan, Yubo The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

On the basis of the conventional PID control algorithm, a modified adaptive PID (MA-PID) control algorithm is presented to improve the steady-state and dynamic performance of closed-loop systems. The proposed method has a straightforward structure without excessively increasing the complexity and cost. It can adaptively adjust the values of the control parameters ($K_p$, $K_i$ and $K_d$) by following a new control law. Simulation results show that the line transient response of the MA-PID is better than that of the adaptive digital PID because the differential coefficient $K_d$ is introduced to changes. In addition, experimental results based on a FPGA indicate that the MA-PID control algorithm reduces the recovery time by 62.5% in response to a 1V line transient, 50% in response to a 500mA load transient, and 23.6% in response to a steady-state deviation, when compared with the conventional PID control algorithm.

Improving Application Performance of in situ Polymerization and Crosslinking System of Maleic Acid/Itaconic Acid for Cotton Fabric

Zhangmi Huang,Xiang Zhou,Zhiqi Xing,Bijia Wang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.2

Maleic acid (MA) and itaconic acid (IA) used as crosslinking agents for cotton fabrics are more cost-effective than the most efficient nonformaldehyde crosslinker 1,2,3,4-butanetetracarboxylic acid (BTCA), but poor stability of finishing bath and fabric yellowing are the main disadvantage of MA/IA in situ polymerization and crosslinking system. In this research, the application performance improvement of MA/IA crosslinking system for cotton fabrics was studied. Replacement of the widely used sodium hypophosphite (SHP) with potassium hypophosphite (PHP) as catalyst allowed for obtaining a stable finishing bath under ambient temperature and led to improved final durable press (DP) performance of the treated fabrics. The influences of PHP concentration, curing temperature, and curing time on the performance of finished fabrics were investigated. Cotton fabrics treated by MA/IA/PHP crosslinking system exhibited comparable DP performance and laundering durability to that finished with BTCA. To address the fabric yellowing problem, the residual MA and IA attached on the treated fabrics by single-ended ester linkage was determined by HPLC. The data indicated that the degree of fabric yellowing was linearly related to the unpolymerized carboxylic acid MA and IA concentration on the treated fabrics. Several approaches were explored to improve the whiteness of MA/IA/PHP crosslinked fabrics. It was found that steam drying with 30-50 % humidity could effectively improve fabric whiteness. The findings of this study have significant implications for better application of unsaturated polycarboxylic acids in crosslinking of cellulose.

Experimental Study of Adaptive Sliding Mode Control for Vibration of a Flexible Rectangular Plate

Yang, Jingyu,Liu, Zhiqi,Cui, Xuanming,Qu, Shiying,Wang, Chu,Lanwei, Zhou,Chen, Guoping The Korean Society for Aeronautical and Space Scie 2015 International Journal of Aeronautical and Space Sc Vol.16 No.1

This paper aims to address the intelligent active vibration control problem of a flexible rectangular plate vibration involving parameter variation and external disturbance. An adaptive sliding mode (ASM) MIMO control strategy and smart piezoelectric materials are proposed as a solution, where the controller design can deal with problems of an external disturbance and parametric uncertainty in system. Compared with the current 'classical' control design, the proposed ASM MIMO control strategy design has two advantages. First, unlike existing classical control algorithms, where only low intelligence of the vibration control system is achieved, this paper shows that high intelligent of the vibration control system can be realized by the ASM MIMO control strategy and smart piezoelectric materials. Second, the system performance is improved due to two additional terms obtained in the active vibration control system. Detailed design principle and rigorous stability analysis are provided. Finally, experiments and simulations were used to verify the effectiveness of the proposed strategy using a hardware prototype based on NI instruments, a MATLAB/SIMULINK platform, and smart piezoelectric materials.

An FPGA-Based Modified Adaptive PID Controller for DC/DC Buck Converters

Ling Lv,Changyuan Chang,Zhiqi Zhou,Yubo Yuan 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

On the basis of the conventional PID control algorithm, a modified adaptive PID (MA-PID) control algorithm is presented to improve the steady-state and dynamic performance of closed-loop systems. The proposed method has a straightforward structure without excessively increasing the complexity and cost. It can adaptively adjust the values of the control parameters (Kp, Ki and Kd) by following a new control law. Simulation results show that the line transient response of the MA-PID is better than that of the adaptive digital PID because the differential coefficient Kd is introduced to changes. In addition, experimental results based on a FPGA indicate that the MA-PID control algorithm reduces the recovery time by 62.5% in response to a 1V line transient, 50% in response to a 500mA load transient, and 23.6% in response to a steady-state deviation, when compared with the conventional PID control algorithm.

Research Progress of the Structure Vibration-Attitude Coordinated Control of Spacecraft

Yang, Jingyu,Qu, Shiying,Lin, Jiahui,Liu, Zhiqi,Cui, Xuanming,Wang, Chu,Zhang, Dujiang,gu, Mingcheng,Sun, Zhongrui,Yang, Kang,Zhou, Lanwei,Chen, Guoping The Korean Society for Aeronautical and Space Scie 2015 International Journal of Aeronautical and Space Sc Vol.16 No.4

This paper gives an overview of research on the field of structure vibration-attitude coordinated control of spacecraft. First of all, the importance of the technology has been given an introduction, and then later the research progress of space structure dynamics modeling, research progress of structure vibration-attitude coordinated control of flexible spacecraft have been discussed respectively. Finally, future research on application of structure vibration-attitude coordinated control of spacecraft has been recommended.

Research Progress of the Structure Vibration-Attitude Coordinated Control of Spacecraft

Jingyu Yang,Shiying Qu,Jiahui Lin,Zhiqi Liu,Xuanming Cui,Chu Wang,Dujiang Zhang,Mingcheng gu,Zhongrui Sun,Kang Yang,Lanwei Zhou,Guoping Chen 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.4

This paper gives an overview of research on the field of structure vibration-attitude coordinated control of spacecraft. First of all, the importance of the technology has been given an introduction, and then later the research progress of space structure dynamics modeling, research progress of structure vibration-attitude coordinated control of flexible spacecraft have been discussed respectively. Finally, future research on application of structure vibration-attitude coordinated control of spacecraft has been recommended.

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Hao Liu,Zhengjun Liu,Wenpeng Dong,Zhiqi Lin,Jingbo Lu,Heng Wan,Zhongxin Zhou 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2

The migration and proliferation of vascular smooth muscle cells (VSMCs) are essential elements during the development of atherosclerosis and restenosis. An increasing number of studies have reported that extracellular matrix (ECM) proteins, including the CCN protein family, play a significant role in VSMC migration and proliferation. CCN4 is a member of the CCN protein family, which controls cell development and survival in multiple systems of the body. Here, we sought to determine whether CCN4 is involved in VSMC migration and proliferation. We examined the effect of CCN4 using rat cultured VSMCs. In cultured VSMCs, CCN4 stimulated the adhesion and migration of VSMCs in a dose-dependent manner, and this effect was blocked by an antibody for integrin 51. CCN4 expression was en-hanced by the pro-inflammatory cytokine tumor necrosis factor  (TNF-). Furthermore, knockdown of CCN4 by siRNA significantly inhibited the VSMC proliferation. CCN4 also could up-regulate the expression level of marker proteins of the VSMCs phenotype. Taken together, these results suggest that CCN4 is involved in the migration and proliferation of VSMCs. Inhibition of CCN4 may provide a promising strategy for the prevention of restenosis after vascular interventions.