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Zhihong Gao,Xiaoyan Luo,Ting Shi,Bin Cai,Zhen Zhang,Zongming Cheng,Weibing Zhuang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.3
MicroRNAs are a class of small, endogenous, non-coding RNA molecules that negatively regulate gene expression at the transcriptional or the post-transcriptional level. Although a large number of miRNAs have been identified in many plant species, especially from model plants and crops, they remain largely unknown in peach. In this study, 110 potential miRNAs belonging to 37 families were identified using computational methods. A total of 43 potential targets were found for 21 families based on near-perfect or perfect complementarity between the plant miRNA and the target sequences. A majority of the targets were transcription factors which play important roles in peach development. qRT-PCR analysis of RNA samples prepared from different peach tissues for 25 miRNA families revealed that miRNAs were differentially expressed in different tissues. Furthermore, two target genes were experimentally verified by detection of the miRNA-mediated mRNA cleavage sites in peach using RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-RACE). Finally, we studied the expression pattern of the two target genes in three different tissues of peach to further understand the mechanism of the interaction between miRNAs and their target genes.
P-Selectin-mediated Acute Inflammation Can Be Blocked by Chemically Modified Heparin, RO-Heparin
Yanguang Gao,Na Li,Rui Fei,Zhihong Chen,Sheng Zheng,Xianlu Zeng 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.3
Selectins are carbohydrate-binding cell adhesion molecules that play a major role in the initiation of inflammatory responses. Heparin can bind to P-selectin, and its anti-inflammatory property is mainly due to inhibition of P-selectin. However, the strong anticoagulant activity of heparin limits its clinical use. We prepared periodate-oxidized, borohydride-reduced heparin (ROheparin) by chemical modification and tested its anticoagulant and anti-inflammatory activities. Activated partial thromboplastin time (aPTT) assays showed that, compared with heparin, RO-heparin had greatly reduced anticoagulant activity. Intravenous administration of this compound led to reduction in the peritonealinfiltration of neutrophils in a mouse acute inflammation model. In vitro cell adhesion experiments demonstrated that the effect of RO-heparin on inflammatory responses was mainly due to inhibiting the interaction of P-selectin with its ligands. These results indicate that RO-heparin may be a safer treatment for inflammation than heparin, especially when selectin is targeted.
Ultrafast epitaxial growth of metre-sized single-crystal graphene on industrial Cu foil
Xu, Xiaozhi,Zhang, Zhihong,Dong, Jichen,Yi, Ding,Niu, Jingjing,Wu, Muhong,Lin, Li,Yin, Rongkang,Li, Mingqiang,Zhou, Jingyuan,Wang, Shaoxin,Sun, Junliang,Duan, Xiaojie,Gao, Peng,Jiang, Ying,Wu, Xiaoson Elsevier 2017 Science bulletin Vol.62 No.15
Scutellaria baicalensis Inhibits Coxsackievirus B3-Induced Myocarditis Via AKT and p38 Pathways
( Qiang Fu ),( Lu Gao ),( Xiao Fu ),( Qinghua Meng ),( Zhihong Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8
Scutellaria baicalensis Georgi has been widely used in China for treatment of various diseases. This study investigated the effect of Scutellaria baicalensis Georgi extracts (SBE) against Coxsackievirus B3 (CVB3)-induced myocarditis in vitro and in vivo. In vitro, Hela cells and primary myocardial cells were infected with CVB3 and treated with SBE (50-800 μg/ml) and ribavirin (200 μM) for 48 h and then determined by CCK8 assay. Real-time PCR and western blotting assays were performed. In vivo, a myocarditis model was induced in male BALB/c mice by injecting CVB3 suspension intraperitoneally for three times, followed by treatment with SBE (400 and 200 mg/kg) and ribavirin (100 mg/kg) for 28 days. SBE ameliorated the cytotoxicity of CVB3 in Hela cells, especially at 400 μg/ml (39.93% vs 65.67%, p < 0.05) without influencing cell growth and also significantly reduced CVB3 replication in primary myocardial cells. The levels of AKT, ERK, and p38 were increased after CVB3 infection. SBE could downregulate the expressions of AKT and p38. In vivo, the mortality rate from CVB3 reached to 66.67%, while 10.00% and 23.33% of this came after 400 and 200 mg/kg SBE treatment, respectively (p < 0.05). The CVB3 replication was obviously reduced after SBE administration from day 5. Similarly, the levels of AKT, ERK, and p38 mRNAs and proteins were increased, and SBE suppressed the expression of AKT and p38. Our study indicates that SBE is a promising potent antiviral agent against CVB3-induced myocarditis by inhibition of virus replication via depressing AKT and p38 expressions.
Security Analysis of the Whirlpool Hash Function in the Cloud of Things
( Wei Li ),( Zhiyong Gao ),( Dawu Gu ),( Chenyu Ge ),( Linfeng Liao ),( Zhihong Zhou ),( Ya Liu ),( Zhiqiang Liu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.1
With the advancement and deployment of leading-edge telecommunication technologies for sensing and collecting, computing related information, Cloud of Things (CoTs) has emerged as a typical application platform that is envisioned to revolutionize the daily activities of human society, such as intelligent transportation, modern logistics, food safety, environmental monitoring, etc. To avoid any possible malicious attack and resource abuse, employing hash functions is widely recognized as one of the most effective approaches for CoTs to achieve message integrity and data authentication. The Whirlpool hash function has served as part of the joint ISO/IEC 10118-3 International Standard by the International Organization for Standardization (ISO) and the International Electrotechnical Commission (IEC). In this paper, we propose an effective differential fault analysis on Whirlpool in the byte-oriented random fault model. The mathematical analysis and experimental results show that 8 random faults on average are required to obtain the current 512-bit message input of whirlpool and the secret key of HMAC-Whirlpool. Our work demonstrates that Whirlpool and HMAC-Whirlpool are both vulnerable to the single byte differential fault analysis. It provides a new reference for the security analysis of the same structure of the hash functions in the CoTs.
Hantao Li,Jie Gao,Ting Shi,Shahid Iqbal,Zhaojun Ni,Zhihong Gao 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.5
Frigida genes play a key role in regulating fl owering time in plants. In this study, we identifi ed 12 PmFRL genes and namedthem according to their chromosomal positions. These genes are unevenly distributed on fi ve chromosomes. Phylogeneticanalysis of Frigida-like proteins of four Rosaceae species and Arabidopsis thaliana showed that these proteins could bedivided into fi ve subfamilies (I–V), and subfamily I ( FRI ) was absent in Rosaceae species. To further reveal the potentialfunctions of these proteins, we performed duplication analysis and found three tandem duplication events and one segmentalduplication event. Tandem duplication promoted the expansion and evolution of the PmFRL genes. RNA-seq analysis showedthat the PmFRL genes were expressed in diff erent tissues, but there were signifi cant diff erences in the expression of somegenes during dormancy. RT-qPCR results supported the RNA-seq results, suggesting that PmFRL genes may be involvedin the regulation of dormancy in Japanese apricot. In conclusion, our comprehensive analysis provides information on theevolution and possible functions of PmFRL genes, laying a foundation for further research on the PmFRL family.
Ni Xiaojia,Iqbal Shahid,Xue Song,Ni Zhaojun,Huang Yinghong,Gao Zhihong 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6
Fruit color is an important factor that aff ects its quality. The red-skinned cultivars of Japanese apricot ( Prunus mume Sieb. et Zucc.) have high medicinal economic benefi ts and are mainly used for deep-processed products because of their eye-catching red pigmentation. In contrast, molecular markers related to the red color trait in Japanese apricot fruit haven’t been reported. This study identifi ed twenty-eight SNPs markers based on genome resequencing data. The average number of alleles per SNP marker site in red and green-skinned groups of Japanese apricot cultivars was 1.9643, with heterozygosity ranging from 0.02 to 0.75, and an average Shannon index of 0.517 and 0.4420, respectively, indicating high dispersion and diversity. The total germplasm was divided into two (K = 2) clusters, including 10 and 34. Most red-skinned Japanese apricot cultivars were classifi ed as cluster I, showing the signifi cant genetic diff erence between the two cultivar groups ( Fst = 0.54). According to PIC values, a total of seven SNPs markers showed high polymorphism ( PIC > 0.5), including PmSNP_1 ( 4-CL ), PmSNP_4 ( WD40 ), PmSNP_5 ( MYB29 ), PmSNP_18 ( UFGT6 ) and PmSNP_27 ( UFGT3 ) that were highly correlated with red color trait. Among them, PmUFGT3 exhibited the highest polymorphism, which is signifi cant for future research on developing and utilizing germplasm resources related to red fruit skin.
Security Analysis of the PHOTON Lightweight Cryptosystem in the Wireless Body Area Network
( Wei Li ),( Linfeng Liao ),( Dawu Gu ),( Chenyu Ge ),( Zhiyong Gao ),( Zhihong Zhou ),( Zheng Guo ),( Ya Liu ),( Zhiqiang Liu ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.1
With the advancement and deployment of wireless communication techniques, wireless body area network (WBAN) has emerged as a promising approach for e-healthcare that collects the data of vital body parameters and movements for sensing and communicating wearable or implantable healthful related information. In order to avoid any possible rancorous attacks and resource abuse, employing lightweight ciphers is most effective to implement encryption, decryption, message authentication and digital signature for security of WBAN. As a typical lightweight cryptosystem with an extended sponge function framework, the PHOTON family is flexible to provide security for the RFID and other highly-constrained devices. In this paper, we propose a differential fault analysis to break three flavors of the PHOTON family successfully. The mathematical analysis and simulating experimental results show that 33, 69 and 86 random faults in average are required to recover each message input for PHOTON-80 /20/16, PHOTON-160/36/36 and PHOTON-224/32/32, respectively. It is the first result of breaking PHOTON with the differential fault analysis. It provides a new reference for the security analysis of the same structure of the lightweight hash functions in the WBAN.
Hayat Faisal,Bai Yang,Iqbal Shahid,Ma Chengdong,Ali Muhammad Moaaz,Shahid Muhammad Adnan,Hasan Mahmood Ul,Mosa Walid F. A.,Khan Ummara,Xiao Huang,Gao Zhihong 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.5
Indole-3-acetic acid (IAA) is an important plant hormone required for various plant growth and developmental activities. YUCCA proteins are the enzymes for IAA synthesis that catalyze the rate-limiting step. However, the YUCCA gene family in Japanese apricot has not been thoroughly characterized. Here, we investigated the eff ect of rootstocks on scion growth of Japanease apricot and genes involved in auxin biosynthesis ( YUCCA ). Growth characteristics revealed that the height of ‘Longyan’ [ Prunus mume Sieb. et Zucc] plants with P. mume rootstock were signifi cantly shorter than ‘Longyan’ plants with P. persica rootstock. This study isolated 13 PmYUCCA genes from the Japanese apricot genome. Bioinformatics and expression studies were undertaken to investigate the functions and characteristics of YUCCA genes. The physicochemical properties, gene structure, conserved domains, conserved motif, and phylogenetic relationships of YUCCA genes were determined using bioinformatics analysis. Likewise, we analyzed the expression of PmYUCCA genes in Japanese apricot’s leaf and stem-bark (scion). The expression levels of PmYUCCA3 and PmYUCCA5 genes were noticeably lower in the leaves of ‘Longyan’/ P. mume graft combination than that of the ‘Longyan’/ P. persica graft combinations, suggesting their distinct roles in regulating growth vigor. This research explores the genome-wide identifi cation, characterization, and possible relationship between growth vigor and expression profi le analysis of the YUCCA gene family in Japanese apricot.