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Zhe-Long Jin,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Meiotic oocytes lack classic centrosomes; therefore, bipolar-spindle assembly depends on the clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. Tumor suppressor P53-binding protein 1 (TP53BP1) is a known mediator in DNA damage response. Recent studies have revealed a new role of TP53BP1 in maintaining centrosome integrity in mitosis. However, the role of TP53BP1 in oocyte meiosis is unclear. Our results show that TP53BP1 participates in DNA damage responses in oocyte, as well as stablishment of chromosome alignment and spindle bipolarity by controlling the clustering of MTOCs during oocyte maturation. TP53BP1 was localized in the cytoplasm and concentrated around the spindle/chromosome region. Knockdown of TP53BP1 induced abnormal DNA damage response involving RAD51 and formation of γH2AX foci. TP53BP1 was also required for the clustering of MTOCs into two spindle poles during pro-meiosis I. Deletion of TP53BP1 induced perturbation in MTOC-localized Aurora Kinase A (AURKA). The knockdown of TP53BP1 induced microtubules to dis-attach from the kinetochores and increased the rate of aneuploidy. Taken together, our data show that TP53BP1 plays crucial roles in chromosome stability and spindle bipolarity during meiotic maturation by regulating the clustering of MTOCs.
JIN, Zhe-Long,KIM, Nam-Hyung 家畜繁殖硏究所 2017 Journal of Reproduction and Development Vol.63 No.5
<P> DNA repair protein RAD51 homolog 1 (RAD51) plays a central role in homologous recombination (HR) repair of DNA breaks. HR depends on the formation of a RAD51 recombinase filament that facilitates strand invasion. However, the role of RAD51 during porcine oocyte maturation is unknown. The objective of this study was to investigate the expression and function of RAD51 during porcine oocyte maturation <I>in vitro</I>. RAD51 was mainly localized to the nucleus at the germinal vesicle (GV) stage, and was widely distributed in the cytoplasm between the GV breakdown (GVBD) and metaphase II stage. DNA damage induced by etoposide was accompanied by the formation of RAD51 foci that were colocalized with γH2AX. Inhibition of RAD51 increased DNA damage and induced metaphase I arrest along with spindle defects, chromosomal misalignment, and abnormal spindle assembly checkpoint (SAC) activity. Inhibition of RAD51 also increased ROS levels and led to an abnormal mitochondrial distribution. Our results indicate that RAD51 plays a critical role in maintaining chromosome integrity and mitochondrial activity during porcine oocyte maturation.</P>
Inhibition of DNA repair protein RAD51 affects porcine preimplantation embryo development
Jin, Zhe-Long,Shen, Xing-Hui,Shuang, Liang,Kwon, Jeong-woo,Seong, Min-Jeong,Kim, Nam-Hyung BioScientifica 2019 Reproduction Vol.157 No.3
<P>Homologous recombination (HR) plays a critical role in facilitating replication fork progression when the polymerase complex encounters a blocking DNA lesion, and it also serves as the primary mechanism for error-free DNA repair of double-stranded breaks. DNA repair protein RAD51 homolog 1 (RAD51) plays a central role in HR. However, the role of RAD51 during porcine early embryo development is unknown. In the present study, we examined whether RAD51 is involved in the regulation of early embryonic development of porcine parthenotes. We found that inhibition of RAD51 delayed cleavage and ceased development before the blastocyst stage. Disrupting RAD51 activity with RNAi or an inhibitor induces sustained DNA damage, as demonstrated by the formation of distinct γH2AX foci in nuclei of four-cell embryos. Inhibiting RAD51 triggers a DNA damage checkpoint by activating the ataxia telangiectasia mutated (ATM)-p53-p21 pathway. Furthermore, RAD51 inhibition caused apoptosis, reactive oxygen species accumulation, abnormal mitochondrial distribution and decreased pluripotent gene expression in blastocysts. Thus, our results indicate that RAD51 is required for proper porcine parthenogenetic activation (PA) embryo development.</P>
Zhe-Long Jin,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
DNA repair protein Rad51 homolog 1 (Rad51) plays a central role in Homologous recombination (HR) repair. HR depends on the formation of a Rad51 recombinase filament that facilitates strand invasion. However, whether Rad51 influences the porcine oocytes during maturation is unknown. The objective of this study is to investigate the expression and function of Rad51 during porcine oocytes maturing in vitro. Rad51 was mainly localized at the nuclear in the germinal vesicle (GV) stage and widely distributed in the cytoplasm form GV breakdown (GVBD) to MII stage. DNA damage inducing by the Etoposide was identified by the Rad51 foci formation which was localized with γH2AX. Inhibition of Rad51 increased the DNA damage, and induced metaphase I arrest with spindle defects, chromosomal misalignments and abnormal spindle assembly checkpoint (SAC) activity. Inhibition of Rad51 also increase ROS level and induced the abnormal mitochondrial distribution. Our results indicate that Rad51 play a critical role to maintain chromosome integrity as well as mitochondria activity during the porcine oocyte maturation.
김용철(Jin Long Zhe),문연희(Moon, Younhee),박만석(Park, Man-seok),최지호(Choi, Jiho) 한국상품학회 2010 商品學硏究 Vol.28 No.6
본 연구는 중국 사회의 문화적 변수인 관시가 영업사원의 신용 및 기업 충성도에 미치는 영향을 살펴봄과 동시에 관시가 기업충성도에 영향을 미침에 있어 신용의 매개효과 및 관시의 직접 효과를 검증해보고자 하였다. 분석 결과, 자동차 산업 및 보험업 등 전 산업에서 관시와 신용간의 관계, 신용과 기업충성도간 관계, 관시와 기업충성도간 관계가 유의하게 나타났으며, 이와 함께 신용의 부분매개 효과가 검증됨으로써 소비자와 영업사원간 관시가 기업 충성도에 직접적인 영향을 미칠 수 있음을 알 수 있었다. This study attempts to empirically test the caual relationships among Guanxi, Xinhong, and loyalty. Six different structural equation models, which specify different relationships among Guanxi, Xinhong, and loyalty, have been developed. Using a data collected from two industries(insurance and automotive) in china, alternative research models are tested. Statistical analysis shows that Guanxi has not only a direct effect but also indirect effect(mediated by Xiyoung) on loyalty. Xiyoung directly impacts the loyalty and is partiallymediated by Guanxi.
B2C 시장에서 기업 신뢰와 판매원 신뢰의 탐색적 연구
김용철(Zhe, Jin Long),문연희(Moon, Youn Hee),박준수(Park, Junsu),최지호(Choi, Jiho) 한국상품학회 2011 商品學硏究 Vol.29 No.3
본 연구는 B-to-B 관계에서 핵심 개념으로 연구되어온 신뢰를 B-to-C 시장으로 확장한 후, 북경과 상해를 대상으로 지역문화에 따라 신뢰역할이 어떻게 달라지는가를 탐색적으로 분석하였다. 신뢰는 그 대상에 따라 기업신뢰와 판매원신뢰로 구분한 후 각각의 대상 별 신뢰 차원이 충성도에 미치는 영향과 대상별 신뢰 간의 상호관계, 그리고대상별신뢰형성요인을실증하였다.분석결과, B-to-B관계에서 제시된 신뢰 효과는 B-to-C관계에서도 유사한 결과를 보여줌과 동시에 상이한 결과 또한 보여주고 있는것 으로나타났다. 이러한 결과에 대한 시사점과 향후 연구가 제시되었다. This paper is an exploratory examination of the impact of firm and its salesperson trust on loyalty in business-consumer relationship. Using data collected in Beijing and Shanghai in China, the authors test researchmodel froma comparative perspective of a cross-regional culture. Our results showthat like B-to-B relationship, firmtrust and salesperson trust in B-to-Cmarket have a central role in building loyalty. However, the factors that invoke the trust-building process and loyalty in B-to-C market differ from those of B-to-B relationship. The authors discuss the implications of the findings for customer relationship management and future research directions.
Liang, Zhe Long,Kang, Kyeongah,Yoon, Sukjoon,Huang, Song Mei,Lim, Jae Sung,Kim, Jin Man,Lim, Jong-Seok,Lee, Hyo Jin Raven Press 2012 Annals of Surgical Oncology Vol.19 No.8
<P>Although NDRG2 is a candidate tumor suppressor, its exact role in renal cell carcinoma (RCC) is not fully understood. We investigated the functional role of NDRG2 and its clinical relevance in RCC tumorigenesis.</P>
( Hai Yan Zhao ),( Hui Ying Li ),( Jian Jin ),( Ji Zhe Jin ),( Long Ye Zhang ),( Mei Ying Xuan ),( Xue Mei Jin ),( Yu Ji Jiang ),( Hai Lan Zheng ),( Ying Shun Jin ),( Yong Jie Jin ),( Bum Soon Choi ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0
Background/Aims: Accumulating evidence indicates that L-carnitine (LC) protects against multiorgan damage through its antioxidant properties and preservation of the mitochondria. Little information is available about the effects of LC on renal fibrosis. This study examined whether LC treatment would provide renoprotection in a rat model of unilateral ureteral obstruction (UUO) and in vitro. Methods: Sprague-Dawley rats that underwent UUO were treated daily with LC for 7 or 14 days. The influence of LC on renal injury caused by UUO was evaluated by histopathology, and analysis of gene expression, oxidative stress, mitochondrial function, programmed cell death, and phosphatidylinositol 3-kinase (PI3K)/ AKT/forkhead box protein O 1a (FoxO1a) signaling. In addition, H<sub>2</sub>O<sub>2</sub>-exposed human kidney cells (HK-2) were treated with LC. Results: LC treatment inhibited expression of proinflammatory and profibrotic cytokines, and was followed by a significant attenuation of tubulointerstitial inflammation and fibrosis. The increased oxidative stress caused by UUO was associated with mitochondrial dysfunction and excessive apoptosis and autophagy via PI3K/AKT/FoxO1a-dependent signaling, and this was abrogated by administration of LC. In H<sub>2</sub>O<sub>2</sub>-exposed HK-2 cells, LC decreased intracellular production of reactive oxygen species, and suppressed expression of profibrotic cytokines and reduced the number of apoptotic cells. Conclusions: LC protects against the progression of tubulointerstitial fibrosis in an obstructed kidney.
Experimental Study on the Performance of a Bidirectional Hybrid Piezoelectric-Hydraulic Actuator
Jin, Xiao Long,Ha, Ngoc San,Li, Yong Zhe,Goo, Nam Seo,Woo, Jangmi,Ko, Han Seo,Kim, Tae Heun,Lee, Chang Seop The Korean Society for Aeronautical and Space Scie 2015 International Journal of Aeronautical and Space Sc Vol.16 No.4
The piezoelectric-hydraulic actuator is a hybrid device that consists of a hydraulic pump driven by a piezo-stack coupled to a conventional hydraulic cylinder. The actuator is of compact size, but can produce a moderate energy output. Such hybrid actuators are currently being researched and developed in many industrialized countries due to the requirement for high performance and compact flight systems. In a previous study, we designed and manufactured a unidirectional hybrid actuator. However, the blocking force was not as high as expected. Therefore, in this study, we redesigned the pump chamber and hydraulic cylinder and also improved the system by removing the air bubbles. Two different types of piezo-stacks were used. In order to achieve bidirectional capabilities in the actuator, commercial solenoid valves were used to control the direction of the output cylinder. Experimental testing of the actuator in unidirectional and bidirectional modes was performed to examine performance issues related to driving frequency, bias pressure, reed valve thickness, etc. The results showed that the maximum blocking force was measured as 970.2N when the frequency was 185Hz.