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- 저자 : Zhanyong Wei (검색결과 3 건)
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Yuxin Li,Jinhao Nie,Yixin Yang,Peikang Bai,Hongjian Zhang,Zhanyong Zhao,Shouzheng Wei,Jie Cai,Qingfeng Guan 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.2
To improve the high-temperature resistance of the key hot-end parts of the steam turbine, NiCoCrAlY coatings were depositedon a 304 stainless steel substrate by laser cladding. The microstructure and high-temperature oxidation behavior of theNiCoCrAlY coatings were analyzed. The results showed that the NiCoCrAlY coatings contained γ/γ′ and β phases, and themicrostructure was mainly composed of elongated columnar crystals. In addition, after 100 h of oxidation at three differentoxidation temperatures (750, 850 and 950 °C), the coatings showed a relatively low oxidation rate, which was approximatelya quarter of the oxidation rate of the substrate. At the same time, the protective Cr2O3scales were formed on the coatingsurface. When the oxidation temperature was 850 °C, the FeCr2O4spinel formed and internal oxidation zone appeared,when the oxidation temperature reached 950 °C, the FeCr2O4spinel gathered in the local area on the surface of the Cr2O3scale and the internal oxidation was aggravated. In other words, as the oxidation temperature increased, the Fe element inthe matrix formed the FeCr2O4spinel, which accelerated the consumption of Cr element in the coatings and reduced theoverall oxidation resistance of the NiCoCrAlY coatings.
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Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway
Xiaohui Jin,Yixin Yuan,Chi Zhang,Yong Zhou,Yue Song,Zhanyong Wei,Gaiping Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3
Background: Porcine parvovirus (PPV) is a single-stranded DNA virus that causes porcine reproductive failure. It is of critical importance to study PPV pathogenesis for the prevention and control of the disease. NS1, a PPV non-structural protein, is participated in viral DNA replication, transcriptional regulation, and cytotoxicity. Our previous research showed that PPV can activate nuclear factor kappa B (NF-κB) signaling pathway and then up-regulate the expression of interleukin (IL)-6. Objectives: Herein, the purpose of this study is to determine whether the non-structural protein NS1 of PPV also has the same function. Methods: Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay, western blot, immunofluorescence assay and small interfering RNA (siRNA) were used. Results: Our findings demonstrated that PPV NS1 protein can up-regulate the expression levels of IL-6 and tumor necrosis factor-alpha in a dose-dependent manner. Moreover, PPV NS1 protein was found to induce the phosphorylation of IκBα, then leading to the phosphorylation and nuclear translocation of NF-κB. In addition, the NS1 protein activated the upstream pathways of NF-κB. Meanwhile, TLR2-siRNA assay showed TLR2 plays an important role in the activation of NF-κB signaling pathway induced by PPV-NS1. Conclusions: These findings indicated that PPV NS1 protein induced the up-regulated of IL-6 expression through activating the TLR2 and NF-κB signaling pathways. In conclusion, these findings provide a new avenue to study the innate immune mechanism of PPV infection.
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Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro
Xiuli Liang,Xiaojun Zhang,Kaiqi Lian,Xiuhua Tian,Mingliang Zhang,Shiqiong Wang,Cheng Chen,Cunxi Nie,Yun Pan,Fangfang Han,Zhanyong Wei,Wen-Ju Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.5
Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 μg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10TCID50 of 62.5 μg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 μg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.
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