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- 저자 : Zhankui Wang (검색결과 2 건)
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Wang Xinzhe,Li Siqi,Yu Honglian,Lv Jianzhi,Fan Minglun,Wang Ximing,Wang Xin,Liang Yanting,Mao Lingna,Zhao Zhankui 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3
BACKGROUND: Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues. METHODS: In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67. RESULTS: The scaffolds had a microporous structure with an appropriate pore size (about 80 lm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity. CONCLUSION: Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.
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A Novel Technique for Dressing Fixed Abrasive Lapping Pad with Abrasive Water Jet
Zhankui Wang,Yakun Yang,Zhao Zhang,Minghua Pang,Mingchao Liang,Lijie Ma,Jianxiu Su 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.6
The dressing method and dressing quality of the fixed abrasive lapping pad significantly impact the processing efficiency and surface quality of the workpiece material. Aiming at the problems of poor dressing effect and high damage degree of the lapping pad in the existing fixed abrasive dressing technology, a new technology using abrasive water jet for dressing is proposed. The self-developed abrasive water jet system was used to conduct a dressing test on a fixed abrasive lapping pad with a copper content of 30%. The influence of jet pressure, nozzle target distance, abrasive concentration and injection angle on the dressing effect were discussed, and the influence mechanism was analyzed by discrete element simulation. The results show that the dressing effect of the abrasive water jet is good. The dressing quality first increases and then decreases with the increase of jet pressure, nozzle target distance and injection angle, and increases with the increase of abrasive concentration. The influence mechanism of each parameter is closely related to the residual height of the lapping pad surface after dressing. The higher the residual height, the rougher the lapping pad surface, and the better the dressing effect of the lapping pad.
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