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- 저자 : Zhang Zengrong (검색결과 3 건)
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Xiaming Chen,Xiaonan Wang,Pengchen Huan,Zengrong Hu,Zhikang Wu,Bo Zhang,Hiromi Nagaumi 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.2
The difference in droplet transfer behavior between the traditional cold metal transfer plus pulse (CMT + P) process and thenew CMT mix synchro-pulse (CMT + SP) process, and its effect on the overall morphology, porosity, microstructure andmechanical properties of WAAM-ed AA2219 alloy are studied. Compared to the pulse stage (fP = 160 Hz) of the traditionalCMT + P process, the pulse stage of CMT + SP process consists of the low-frequency pulse and high-frequency pulse (HFP)stages. The high frequency (fH = 200 Hz) of the HFP stages formed a high arc force, enlarging the molten pool and enhancingthe convection of molten pool. This increased the melting depth between passes and facilitated the flow of the moltenpool to the sides and escaping of the pores. As a result, a highly symmetric WAAM-ed AA2219 alloy with a high effectiveheight (14.2 mm) and effective area (89.6 mm2),and low porosity (0.6 ± 0.2%) was manufactured by the CMT + SP process. Moreover, the fine grains (61 μm) with a 43% (area fraction) equiaxed grains were obtained with the CMT + SP process,leading to more θ′ phases precipitating. Thus, the average microhardness and tensile strength of the CMT + SP samplesincreased to 80.2 ± 6.7 HV and 265.0 ± 17.0 MPa, respectively.
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Wenwu Zhou,Xin Yuan,Ping Qian,Jiaan Cheng,Chuanxi Zhang,Geoff Gurr,Zengrong Zhu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4
Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5)weremore highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.
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Qiu Mohan,Yu Chunlin,Zhu Shiliang,Liu Siyang,Peng Han,Xiong Xia,Chen Jialei,Jiang Xiaosong,Du Huarui,Li Qingyun,Zhang Zengrong,Yang Chaowu 한국유전학회 2022 Genes & Genomics Vol.44 No.11
Background: Long non-coding RNAs (lncRNAs) play an essential role in biological processes. However, the expression patterns of lncRNAs that regulate the non-Mendelian inheritance feather phenotypes remain unknown. Objective: This study aimed to compare the expression profiles of lncRNAs in the follicles of the late-feathering cocks (LC) and late-feathering hens (LH) that followed genetic rules and the early-feathering hen (EH) and early-feathering cock (EC) that did not conform to the genetic laws. Methods: We performed RNA sequencing and investigated the differentially expressed lncRNAs (DElncRNAs) between the early- and late-feathering chickens, which function by cis-acting or participate in the competing endogenous RNA (ceRNA) network. Results: A total of 53 upregulated and 43 downregulated lncRNAs were identified in EC vs. LC, and 58 upregulated and 109 downregulated lncRNAs were identified in EH vs. LH. The target mRNAs regulated by lncRNAs in cis were enriched in the pentose phosphate pathway, TGF-β signaling pathway and Jak-STAT signaling pathway in EC vs. LC and were associated with the TGF-β signaling pathway, Wnt signaling pathway, p53 signaling pathway and Jak-STAT signaling pathway in EH vs. LH. In addition, the lncRNA-mediated ceRNA regulatory pathways of hair follicle formation were mainly enriched in the TGF-β signaling pathway, Wnt signaling pathway, melanogenesis, and calcium signaling pathways. The levels of ENSGALG00000047626 were significantly higher in the late-feathering chickens than in the early-feathering chickens, which regulated the expression of SSTR2 by gga-miR-1649-5p. Conclusion: This study provides a novel molecular mechanism of lncRNA's response to the feather rate that does not conform to the genetic laws in chickens.
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