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- 저자 : Zhang Yunrui (검색결과 2 건)
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A transcription factor TaTCP20 regulates the expression of Ppd-D1b in common wheat
Wei Fan,Song Tianqi,Zhou Jianfei,Cheng Jie,Li Ruibo,Yu Ming,Zhang Yunrui,Yu-Yang Song,Zhang Bo,Zhang Xiaoke 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3
Photoperiod (Ppd) genes play an important role in the adaptation of wheat to the ecological environment. However, the transcriptional regulation mechanism of photoperiodic genes has remained elusive. This study isolated a full-length promoter of Ppd-D1b (2518 bp) from the common wheat genome. Several essential core cis-acting elements and numerous light-responsive cis-acting regulatory elements were identifed in Ppd-D1b promoter by the in-silico analysis. Ten 5’-deleted length fragments of the Ppd-D1b promoter fused with GUS were constructed and named D0 ~D9, then transferred them into Arabidopsis thaliana. GUS gene driven by full-length (D0) in transgenic Arabidopsis thaliana showed the same rhythm with Ppd-D1b in wheat under short-day conditions (SDs, 8-h light/16-h dark). The expression of GUS gene in D0 reached its peak at 3 h after dawn, then decreased to the lowest and remained stable. Analysis of the series of 5’-deleted fragments showed that at 3 h after dawn, GUS gene expression activity decreased signifcantly in D7a due to removal of CHEBS (CCA1 HIKING EXPEDITION binding site). Moreover, yeast one-hybrid (Y1H) and dual-luciferase (dual-LUC) assays revealed that TaTCP20 could bind to the Ppd-D1b promoter to increase its transcriptional activity. This study revealed a transcription factor, TaTCP20, which activated Ppd-D1b by binding to CHEBS, provided a foundation for the theoretical research on wheat’s photoperiodic response mechanism.
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Shiyang Chen,Tongda Lei,Yunrui Zhang,Huancheng Wu,Sen He,Wei Liu,Jie Fan,Yong Liu 한국섬유공학회 2023 Fibers and polymers Vol.24 No.2
Fibrous 3D scaffold with small fiber diameter has the similar topographic and structural characteristics of native extracellularmatrix (ECM), which provides the beneficial microenvironment for cell adhesion, growth, migration, proliferation. However,the pore structure of the biopolymer scaffold is crucial for cell regulation and tissue regeneration in practical application. Inthis report, we proposed a nanofiber induced silk fibroin nanofibers/silk fibroin (SFNF/SF) fibrous scaffold with homogeneousmicron pores using fast freeze-drying technology under -196 °C. The physical, chemical and biological performance of thescaffold was investigated. Ethanol post treatment of the scaffold led to the conformation transition of silk fibroin from randomcoil (silk I) to beta-sheet (silk II) and increase of the crystallinity of the scaffold, which greatly improved the stability of thescaffold in water. Scaffolds made from 2 to 6% SFNF/SF solution with SFNF/SF ratio ranging from 1:1 to 1:8 exhibited threedimensional (3D) fibrous structure with porosity of 80–85% and pore size ranging from 5 to 15 μm due to the entanglementof the nanofibers. And the fibrous structure of the scaffolds can be adjusted by controlling the concentration of the SFNF/SF solution and the SFNF/SF ratio. Cell culture suggested that the 3D fibrous network structure with micron pores showedadvantages for cell migration comparing with the lamella structure scaffold. After 7 day’s culture, cells migrated to about240 μm inside the 6% 1:1 scaffold, while only about 160 μm inside the 6% 1:16 scaffold. The nanofiber induced micro porousSFNF/SF scaffolds by fast freeze-drying technology is potential for preparation of micron porous scaffold.
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