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In silico Discovery of Genes Expressed in Liver, Kidney, Spleen and Small Intestine of Pigs
Pan, Zengxiang,Liu, Honglin,Chen, Jie,Xu, Dan,Jiang, Zhihua,Xie, Zhuang Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.2
An in silico approach was developed to survey the genes expressed in four internal organs of pig: liver, kidney, spleen and small intestine. The major procedures of the approach included: (1) BLAST searching against GenBank "est_others" database using human cDNA sequences as queries to screen the porcine orthologous expressed sequence tags (ESTs), (2) classifying the porcine ESTs records by resources according to certain criteria and (3) analyzing data for ESTs specifically expressed in each organ. In order to do so, four Java programs were developed. Based on the ESTs available in the GenBank database, it was found that there were at least 2,100 genes expressed in these four organs, including 128 in the liver, 81 in the kidney, 780 in the spleen, and 1,423 in the small intestine respectively (a few genes co-expressed in these tissues). Gene expression patterns, such as co-expressed genes, preferentially expressed genes and basic active genes were also compared and characterized among these organs. This study provides a comprehensive model on how to use the bioinformatics approach and Genbank databases to facilitate the discovery of new genes in livestock species.
Pan, Zengxiang,Zhang, Junlei,Zhang, Jinbi,Zhou, Bo,Chen, Jie,Jiang, Zhihua,Liu, Honglin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.7
In Erhualian and Yorkshire reciprocal cross $F_1$ pig populations, we examined the mRNA expression characteristic of liver-derived IGF-1, IGF-1R, IGF-2, IGF-2R and IGFBP-3 during the embryonic and postnatal developmental periods (E50, E70, E90, D1, D20, D70, D120 and D180). Our results demonstrated that the IGF-system genes mRNA levels exhibited an ontogenetic expression pattern, which was potentially associated with the porcine embryonic development, postnatal growth, organogenesis and even the initiation and acceleration of puberty. The expression pattern of IGF-system genes showed variation in the reciprocal cross ($F_1$ YE and EY pigs). This study also involved the expression features of imprinted genes IGF-2 and IGF-2R. The parent-of-origin effect of imprinted genes was reflected by their differential expression between the reciprocal crosses populations. The correlation analysis also indicated that the regulatory network and mechanisms involved in the IGF system were a complex issue that needs to be more fully explored. A better understanding of IGF system components and their interactive mechanisms will enable researchers to gain insights not only into animal organogenesis but also into somatic growth development and even reproduction.
HOW CAN MORALITY-IRRELEVANT INSPIRATION LEAD TO MORALITY BEHAVIOR?
Jianping Liang,Zengxiang Chen,Jing Lei 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07
In this paper, we investigate whether and how morality irrelevant inspiration can influence consumer’s own morality behavior. Thrash & Elliot (2003) conceptualized inspiration as a psychological construct with three core characteristics: evocation, transcendence, and motivation. Morality behavior include prescriptive morality (e.g., prosocial behavior) and proscriptive morality (e.g., immoral behavior), which have different antecedents. We propose a common antecedent for both faces of morality and found that morality-irrelevant inspirational experiences (triggered by others or oneself) could decrease proscriptive morality and increase prescriptive morality, via emotional and cognitive transcendence. This is the first paper to explore the roles played by the emotional and cognitive transcendence resulted from the inspirational experiences and the consequences of transcendence on a source-irrelevant context.