Evaluation of Macro Mineral Contents of Forages: Influence of Pasture and Seasonal Variation

Khan, Zafar Iqbal,Ashraf, M.,Hussian, Altaf Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.6

Concentrations of major elements in forages were determined in relation to ruminant requirements at a livestock experimental station in Leiah district, Punjab-Pakistan using mapping techniques. The study investigated the influence of sampling periods and pasture types on the concentrations of calcium, magnesium, potassium, and sodium in forages. The implications of these forages for nutrition of ruminants were assessed for the livestock population at that particular experimental station, which are supported by the farm pastures. Within the farm, variations in the element status of the forages were related to soil pasture types. A tentative assessment of the mineral status of available forages at this farm for different pastures using guidelines developed for domestic animals indicated deficiencies of only Na, but forages contained adequate Ca, Mg, and K levels required for grazing ruminants. The concentrations of Na in the forage reserves indicated that the potential supply of this element to plants was limited from the soil to plants and from plants to the animals grazing them. Soil minerals were not measured in this study. The potential use of fertilizers, as pasture amendment as well as supplementation of ruminants, with a specifically tailored mineral mixture is important to livestock producers and environmentalists as well, because their use may improve forage nutritive value and in turn meet requirements of animals.

Mineral Status of Soils and Forages in Southwestern Punjab-Pakistan: Micro-minerals

Khan, Zafar Iqbal,Hussain, A.,Ashraf, M.,McDowell, L.R. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.8

This study was conducted to evaluate the trace elemental nutritive values of soil and forages collected from southwestern part of the province of Punjab, Pakistan. Soil and forage samples were collected fortnightly for two seasons. The concentrations of some trace minerals varied greatly among seasons and sampling periods. Seasonal effects were found in all soil micro-minerals except zinc, while forage iron, zinc, and selenium were affected by seasonal changes. Sampling periods effects were observed in all soil minerals and in forage copper, iron, zinc, and manganese only. All soil mineral levels except cobalt and selenium were sufficiently high to meet the requirements of plants for normal growth during both seasons. In contrast, soil Co and Se levels were severely deficient during both seasons and considered inadequate for plant growth. Soil Fe, Zn, Co, and Se levels were higher, and Cu and Mn lower during winter than those during summer. Forage Zn levels during summer were at marginal deficient levels, and in contrast, all other forage micro-minerals were within the required range for ruminants during both seasons. Although forage mico-minerals were within the range required by the ruminants, they were not sufficiently high to prevent the predisposition to various diseases caused by nutrient deficiency. Consequently, grazing animals at this location need continued mineral supplementation of these elements with a mixtures of high bio-availability rather than of high micro-mineral contents to support optimum ruminant productivity.

Effect of antidiarrhoeal formulation on acute experimental diarrhoea in rats

Khan, M. Aleem,Khan, Naeem A.,Qasmi, Iqbal A.,Ahmad, Ghufran,Zafar, Shadab Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.2

Aqueous extract of antidiarrhoeal formulation (ADF) containing Holarrhena antidysentrica, Aegle marmelos and Punica granatum was investigated for antidiarrhoeal activity against charcoal-induced gut transit, serotonin-induced diarrhoea and $PGE_2-induced$ small intestine enteropooling in rats. The control, standard and test groups of experimental animals were administered with normal saline (p.o.), diphenoxylate hydrochloride (5 mg/kg, p.o.) and ADF (250 mg and 500 mg/kg, p.o.) respectively except the control group of $PGE_2-induced$ small intestine enteropooling which received only 5% ethanol in normal saline (i.p.). Charcoal (10 ml/kg, p.o.) and serotonin $(600\;{\mu}g/kg,\;i.p.)$ were administered after 30 min, while $PGE_2\;(100\;{\mu}g/kg,\;p.o.)$ was administered immediately afterwards. The distance traveled by charcoal in small intestine was measured after 15 and 30 min of charcoal administration, diarrhoea was observed every 30 min for six hour after serotonin administration and the volume of intestinal fluid was measured after 30 min of $PGE_2$ administration. Oral administration of ADF significantly inhibited the frequency of defaecation and decreased the propulsion of charcoal meal through the gastrointestinal tract, reduced the wetness of faecal dropping in serotonin-induced diarrhoea and also reduced the $PGE_2-induced$small intestine enteropooling. ADF may have potential to reduce the diarrhoea in rats.

Antidiarrhoeal activity of Arque-Ajeeb, a compound formulation of Unani medicine in rats

Khan, M. Aleem,Khan, Naeem A.,Qasmi, Iqbal A.,Ahmad, Ghufran,Zafar, Shadab Kyung Hee Oriental Medicine Research Center 2004 Oriental pharmacy and experimental medicine Vol.4 No.2

The efficacy of Arque-Ajeeb (AA), a compound formulation of Unani medicine was investigated for antidiarrhoeal activity against barium sulphate-induced gut motility and castor oil-induced diarrhoea in rats. The control, standard and test groups of experimental animals were administered with distilled water (p.o.), lomotil (p.o.) and AA (0.007 ml and 0.014 ml/100 g, p.o.) respectively. Barium sulphate and castor oil were administered after 15 and 60 min respectively in each group of first and second experiment. The distance traveled by barium sulphate in small intestine was measured after 15 and 30 min of barium sulphate administration and diarrhoea was observed every 30-min for six hour after castor oil administration. Oral administration of AA caused significant reduction in diarrhoea. AA may have the potential to reduce the diarrhoea in rats.

A Scalable Data Integrity Mechanism Based on Provable Data Possession and JARs

( Faheem Zafar ),( Abid Khan ),( Mansoor Ahmed ),( Majid Iqbal Khan ),( Farhana Jabeen ),( Zara Hamid ),( Naveed Ahmed ),( Faisal Bashir ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.6

Cloud storage as a service provides high scalability and availability as per need of user, without large investment on infrastructure. However, data security risks, such as confidentiality, privacy, and integrity of the outsourced data are associated with the cloud-computing model. Over the year`s techniques such as, remote data checking (RDC), data integrity protection (DIP), provable data possession (PDP), proof of storage (POS), and proof of retrievability (POR) have been devised to frequently and securely check the integrity of outsourced data. In this paper, we improve the efficiency of PDP scheme, in terms of computation, storage, and communication cost for large data archives. By utilizing the capabilities of JAR and ZIP technology, the cost of searching the metadata in proof generation process is reduced from O(n) to O(1). Moreover, due to direct access to metadata, disk I/O cost is reduced and resulting in 50 to 60 time faster proof generation for large datasets. Furthermore, our proposed scheme achieved 50% reduction in storage size of data and respective metadata that result in providing storage and communication efficiency.

Preventable Lifestyle Risk Factors for Non-Communicable Diseases in the Pakistan Adolescents Schools Study 1 (PASS-1)

Ali Khan Khuwaja,Saleem Khawaja,Komal Motwani,Adeel Akbar Khoja,Iqbal Syed Azam,Zafar Fatmi,Badar Sabir Ali,Muhammad Masood Kadir 대한예방의학회 2011 예방의학회지 Vol.44 No.5

Objectives: The rising burden of preventable risk factors for non-communicable diseases (NCDs) among adolescents is a major public health challenge worldwide. We identified the preventable risk factors for NCDs in adolescents. Methods: In a school-based study, pre-tested structured questionnaires were completed by 414 adolescents (14 to 17years) at six schools in three cities in Pakistan. The chi-squared test and adjusted odds ratio (aOR) with 95% confidence interval (CI) were calculated in a multinomial logistic regression analysis. Results: Over 80% of the adolescents had unhealthy diets, and 54% were physically inactive. Most adolescents were exposed to passive smoking, and 14% were also current smokers. More than one-third of participants chewed betel nut,and one-quarter used oral tobacco. More girls were physically inactive (OR, 4.07; 95% CI, 2.69 to 6.17), whereas a greater proportion of boys were current smokers (OR, 2.17; 95% CI, 1.19 to 3.91), exposed to passive smoking (OR, 2.57;95% CI, 1.72 to 3.83), and using betel nut (OR, 2.03; 95% CI, 1.34 to 3.06). Only 3.1% of the participants were without any preventable lifestyle risk factor for NCDs, and over 80% had ≥2 factors. Co-existence of risk factors was independently associated with fathers being blue-collar workers (aOR, 3.57; 95% CI, 1.07 to 11.92) and parents not treating their child fairly (aOR, 5.05; 95% CI, 1.29 to 19.78). Conclusions: Most of the adolescents studied had preventable risk factors for NCDs. These results warrant comprehensive and integrated interventions to prevent lifestyle risk factors, and parents are front-line stakeholders.

Preventable Lifestyle Risk Factors for Non-Communicable Diseases in the Pakistan Adolescents Schools Study 1 (PASS-1)

Khuwaja, Ali Khan,Khawaja, Saleem,Motwani, Komal,Khoja, Adeel Akbar,Azam, Iqbal Syed,Fatmi, Zafar,Ali, Badar Sabir,Kadir, Muhammad Masood The Korean Society for Preventive Medicine 2011 예방의학회지 Vol.44 No.5

Objectives: The rising burden of preventable risk factors for non-communicable diseases (NCDs) among adolescents is a major public health challenge worldwide. We identified the preventable risk factors for NCDs in adolescents. Methods: In a school-based study, pre-tested structured questionnaires were completed by 414 adolescents (14 to 17 years) at six schools in three cities in Pakistan. The chi-squared test and adjusted odds ratio (aOR) with 95% confidence interval (CI) were calculated in a multinomial logistic regression analysis. Results: Over 80% of the adolescents had unhealthy diets, and 54% were physically inactive. Most adolescents were exposed to passive smoking, and 14% were also current smokers. More than one-third of participants chewed betel nut, and one-quarter used oral tobacco. More girls were physically inactive (OR, 4.07; 95% CI, 2.69 to 6.17), whereas a greater proportion of boys were current smokers (OR, 2.17; 95% CI, 1.19 to 3.91), exposed to passive smoking (OR, 2.57; 95% CI, 1.72 to 3.83), and using betel nut (OR, 2.03; 95% CI, 1.34 to 3.06). Only 3.1% of the participants were without any preventable lifestyle risk factor for NCDs, and over 80% had ${\geq}$2 factors. Co-existence of risk factors was independently associated with fathers being blue-collar workers (aOR, 3.57; 95% CI, 1.07 to 11.92) and parents not treating their child fairly (aOR, 5.05; 95% CI, 1.29 to 19.78). Conclusions: Most of the adolescents studied had preventable risk factors for NCDs. These results warrant comprehensive and integrated interventions to prevent lifestyle risk factors, and parents are front-line stakeholders.

Breast Lymphoma Treatment Outcomes in a Pakistani Population: 20 Years of Experience at a Single Center

Bano, Razia,Zafar, Waleed,Khan, Amina Iqbal,Fiaz, Sohaib Adil,Abid, Mahwish,Chaudhary, Mohammad Zulqarnain,Siddique, Neelam,Khan, Huma Majeed Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7

Background: Breast lymphomas constitute a rare disease entity. To date, limited relevant data have been reported. We therefore here present a review of breast lymphoma patients treated at a single center over a 20 year period, focusing on histological types, treatment modalities and outcomes. Materials and Methods: We identified patients who were diagnosed and treated for breast lymphoma at a single center from January 1995 to January 2014 and extracted data regarding patients' demographics and clinical data. Results: Twenty-seven patients with breast lymphoma were identified, of which 3 were males. The median age at diagnosis was 37 years (range: 22-76 years). Chemotherapy was the main stay of treatment and 55.6% patients also received radiation to the affected breast. At our institute, only 3 patients, all with progressive disease, had surgery performed to achieve local palliation. Complete response after chemotherapy was seen in 63% patients and partial response in 7.4%, while 26% patients demonstrated disease progression. The mean follow up was 46.8 months. Seven patients (33.3%) who were alive at last follow up, as well as 1 patient who died, survived more than 5 years after diagnosis. Conclusions: Patients with breast lymphoma should receive aggressive treatment, with combination of chemotherapy and radiation therapy. Surgery should be limited for diagnosis and palliation of local symptoms in cases of progressive disease.

Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan

Tahira, Bibi,Asif, Muhammad,Khan, Samiullah,Hussain, Abrar,Shahwani, Muhammad Naeem,Malik, Arif,Inayatullah, Syed,Iqbal, Zafar,Rasool, Mahmood Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome. Materials and Methods: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms. Results: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 $10^3/{\mu}l$, hemoglobin 10.44 mg/dl, and platelets 288.6 $10^3/{\mu}l$ were observed in this study. Conclusions: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

Kamran, Muhammad,Javed, Nazir,Ullah, Ihsan,Nazir, Shahid,Jamil, Shakra,Iqbal, Muhammad Zafar,Abbas, Huma,Khan, Sajid Aleem,Haq, Muhammad Ehetisham ul The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.1

A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.