ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1

Liu Na,Feng Yuchen,Liu Huicheng,Wu Wenliang,Liang Yuxia,Li Pingfei,Wei Zhengping,Wu Min,Tang Zhao-Hui,Han Junyan,Cheng Xiang,Liu Zheng,Laurence Arian,Li Huabin,Zhen Guohua,Yang Xiang-Ping 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.3

Purpose Macrophages are important regulators of environmental allergen-induced airway inflammation and asthma. ATP6V0d2 is a subunit of vacuolar ATPase highly expressed in macrophages. However, the functions of ATP6V0d2 in the regulation of pathogenesis of allergic asthma remain unclear. The aim of this study is to determine the function and related molecular mechanisms of macrophage protein ATP6V0d2 in allergic asthma. Methods We compared the disease severity between female C57BL/6 wild-type and ATP6V0d2−/− mice in an ovalbumin (OVA)-induced asthma model. We also investigated the association of expression of ATP6V0d2, PU.1 and CCL17 with disease severity among asthmatic patients. Results The expression of ATP6V0d2 in sputum cells of asthmatic patients and in the lungs of OVA-challenged mice was enhanced compared to healthy subjects and their counterparts, respectively. However, ATP6V0d2-deficient mice exaggerated inflammatory cell infiltration as well as enhanced alternative activated macrophage (AAM) polarization and mucus production in an OVA-induced asthma model. Furthermore, we found that Atp6v0d2 promoted lysosomal degradation of Pu.1, which induced AAM polarization and Ccl17 production. Among asthma patients, ATP6V0d2 expression was inversely associated with disease severity, whereas PU.1 and CCL17 expression was positively associated with disease severity. Conclusions Our results identify macrophage Atp6v0d2, as an induced feedback inhibitor of asthma disease severity by promoting Pu.1 lysosomal degradation, which may in turn leads to reduced AAM polarization and Ccl17 production.

Manganese dioxide-graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Yuxia Liu,Zhenfa Zhang,Cuizong Zhang,Wei Huang,Caiyun Liang,Jinyun Peng 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.8

Excess amount of analgesic and anti-inflammatory drug, such as indomethacin, often leads to serious gastrointestinal complications; therefore, amount of such active compound should be regulated in commercial drugs. This study proposes an efficient analytical technique to detect indomethacin selectively. We prepared and investigated electrochemical properties of a manganese dioxide-graphene nanocomposite film modified glassy carbon electrode (MnO2-Gr/GCE). The behavior of the modified electrode as electrocatalyst towards indomethacin oxidation was also examined. The cyclic voltammetric results reveal that the electrocatalytic activity for the oxidation of indomethacin can significantly be enhanced on the MnO2-Gr/GCE. Indomethacin exhibited a sensitive anodic peak at about 0.90 V at MnO2-Gr/GCE. The data obtained from differential pulse voltammetry showed that the anodic peak currents were linearly dependent on the indomethacin concentrations in the range of 1.0 × 10−7 to 2.5 × 10−5 mol/L with a detection limit of 3.2 × 10−8 mol/L (S/N = 3). Most importantly, the proposed method shows efficient and selective sensing of indomethacin in commercial pharmaceutical formulations. This is the first report of a voltammetric sensor for indomethacin using MnO2-Gr/GCE. We believe that this new method can be commercialized for routine applications in laboratories.

Study on the Brittleness Characteristics of Sand Reinforced with Polypropylene Fiber and Polyurethane Organic Polymer

Jin Liu,Ying Wang,Debi Prasanna Kanungo,Jihong Wei,Yuxia Bai,Ding Li,Zezhuo Song,Yi Lu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.3

The brittleness characteristics of sand reinforced with polypropylene fiber (PF) and polyurethane organic polymer(POP) was investigated in detail using direct tensile and unconfined compression tests. The effects of POP/PF content, curing time and sand density on the brittleness of reinforced sand were studied. The reinforcement mechanism was analyzed with images of scanning electron microscope (SEM) and sample failure mode. The test results indicated that the brittleness of sand increases with curing time, and it has no obvious change after 48 h. The brittleness was decreased obviously with the increase in POP and PF contents. The brittleness of reinforced sand reaches a relative stable state when the POP and fiber contents reach 3 % and 0.6 % respectively. The brittleness also increased with sand density and it reaches a plateau at 1.55 g/cm3. The POP forms a large number of polymer films with water volatilizing in the sand, and the sand particles were wrapped. Fibers improved the compressive strength of reinforced sand and changed it to a more ductile one. Polypropylene fiber plays the role of reinforcing the sand, effectively improving the strength of the sand and reducing the brittleness of the reinforced sand.

Nonlinear Adaptive Backstepping with ESO for the Quadrotor Trajectory Tracking Control in the Multiple Disturbances

Jie Liu,Wendong Gai,Jing Zhang,Yuxia Li 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.11

In this paper, we present a nonlinear adaptive backstepping with extended state observer (ESO) trajectory tracking controller for a quadrotor unmanned aerial vehicle (UAV) subject to the multiple disturbances, which include the parametric uncertainties, actuator faults and external disturbance. First, a six-degrees of freedom quadrotor UAV model with the multiple disturbances function is built. Second, the adaptive backstepping controller is designed to track the desired trajectory command aim at internal disturbance. And the adaptive backstepping controller with ESO is designed to track the desired trajectory command aim at external disturbance. Third, the stability of the system is proved by the circle criterion. Finally, under different flight scenarios, simulation results are given to demonstrate the effectiveness of the proposed method.

Influence of Polyurethane Polymer on the Strength and Mechanical Behavior of Sand-root Composite

Jin Liu,Zhihao Chen,Zhaojun Zeng,Debi Prasanna Kanungo,Fan Bu,Yuxia Bai,Changqing Qi,Wei Qian 한국섬유공학회 2020 Fibers and polymers Vol.21 No.4

Vegetation has good application in slope stabilization, but its beneficial effects on reinforcing topsoil are generallylimited by the soil properties it was cultivated in. This study aims at evaluating the strength improvements of sand-rootcomposite by treating with polyurethane polymer and hence investigating the mechanism of polymer-root-soil interactions. Vegetation roots were selected and mixed with dry sand and polymer solution to prepare remolded specimens. A series ofexperimental tests were then performed at different percentages of root content (0, 0.4, 0.8, 1.2, and 1.6 % by weight of drysand) and polymer content (1, 2, and 4 % by weight of dry sand) to evaluate the shear parameters and unconfinedcompressive strength (UCS). The combined mechanism was studied by scanning electron microscopy (SEM) images. Theresults showed that the strengthening effect has greater efficiency with higher polymer content. Through varying contents ofvegetation root, it was found that low root content induced an undesirable weakening effect on the strength of the treated soil. However, this situation was somewhat improved with the increase in root content. The good flexibility of polymers not onlypromote the capacity of soil to energy absorption, but also impart good ductility to soil. The presence of polymers greatlystrengthens soil stability due to its special network structure, by which the improved shear resistance at the root-soil interfaceprovides sufficient anchorage effect for the tensile strength of roots to be fully mobilized. Overall, the synergistic effect ofroot reinforcement and polymer treatment has the potential for its use in soil stabilization.

Effect of Basalt Fiber on the Strength Properties of Polymer Reinforced Sand

Jin Liu,Yuxia Bai,Zezhuo Song,Ying Wang,Zhihao Chen,Qiongya Wang,Debi Prasanna Kanungo,Wei Qian 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11

This paper displays an experimental study of the effect of basalt fiber on the strength properties of polymer reinforced sand. Laboratory trials of unconfined compression test (UCS), direct shear test, and tensile test were conducted on the specimens treated with polymer and basalt fiber, and several factors including polymer content, fiber content and dry density of sand that will influence the strength behaviors are investigated in detail. Based on test results and scanning electron microscope (SEM) images, the reinforcement mechanism was analyzed. The results showed that the polymer content, basalt fiber content and dry density of sand had greatly improved the strength behaviors of reinforced specimens. The increase in polymer and fiber content had an active effect on strength characteristics, while the angle of internal decreased slightly. The strength properties were enhanced with the increase in dry density, and the effect of dry density on tensile strength is affected by fiber content. The presence of randomly distributed fibers has formed a spatial fiber-sand net in sand, and the additive of polymer solution formed membrane to enwrap sand particles and connect sand and fibers, thereby formed a stable structure in sand. These structures have increased the bonding and interlocking forces between sand and fibers, and decreased the void ratio of reinforced specimens.

Four new species of the genus Rhagonycha (Coleoptera: Cantharidae) from Southeast China

Xiaoyun Feng,Liu Haoyu,Yang Xing-ke,Yang Yuxia 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

Four new species of Rhagonycha Eschscholtz, 1830 are described, including Rh. hainanensis sp. nov. (China, Hainan), Rh. nigroimpressimima sp. nov. (China, Fujian), Rh. tumida sp. nov. and Rh. nigrotibia sp. nov. (China, Guangxi). These new species are illustrated with habitus, aedeagi, abdominal sternites VIII and internal genitalia of females. Besides, the female internal genitalia of Rh. nigroimpressa (Pic, 1922a) is illustrated for the first time to make comparison with the new species. An identification key and a distribution map of those Rhagonycha species with yellowish elytra from southeast China are present.

Five new species of Stenothemus Bourgeois from Guangxi, China (Coleoptera, Cantharidae)

Ge Shujuan,Liu Haoyu,Yang Xing-ke,Yang Yuxia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Five new species of Stenothemus Bourgeois, 1907 from Guangxi, China are described, S. normalis sp. nov., S. flavicans sp. nov., S. nigripennis sp. nov., S. elongaticeps sp. nov. and S. maculaticollis sp. nov.. They are illus trated with habitus photos and aedeagi of males, also abdominal sternites VIII and internal genitalia of females. A key for the identification of the above species is provided.

Uranium thermochemical cycle used for hydrogen production

Aimei Chen,Chunxia Liu,Yuxia Liu,Lan Zhang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.1

Thermochemical cycles have been predominantly used for energy transformation from heat to storedchemical free energy in the form of hydrogen. The thermochemical cycle based on uranium (UTC),proposed by Oak Ridge National Laboratory, has been considered as a better alternative compared toother thermochemical cycles mainly due to its safety and high efficiency. UTC process includes threesteps, in which only the first step is unique. Hydrogen production apparatus with hectogram reactantswas designed in this study. The results showed that high yield hydrogen was obtained, which wasdetermined by drainage method. The results also indicated that the chemical conversion rate ofhydrogen production was in direct proportion to the mass of Na2CO3, while the solid product wasNa2UO4, instead of Na2U2O7. Nevertheless the thermochemical cycle used for hydrogen generation can beclosed, and chemical compounds used in these processes can also be recycled. So the cycle with Na2UO4as its first reaction product has an advantage over the proposed UTC process, attributed to the fast reactionrate and high hydrogen yield in the first reaction step.

A New Closed-loop Control Allocation Method with Application to Direct Force Control

Wendong Gai,Jie Liu,Jing Zhang,Yuxia Li 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.3

A new closed-loop control allocation method is proposed to distribute the force and moment increment commands among multiple actuators at the same time. An innovative feedback configuration for the force and moment increment produced by the actuators deflections is added into the trajectory and attitude control loop, respectively. In addition, the trajectory and attitude controllers designed by nonlinear dynamic inversion are used to derive the desired force and moment increment commands. Then, the stability of the closed-loop system is guaranteed theoretically when the actuators are healthy and fail, where there is no a fault detection method to identify the failure. Finally, the proposed method is used to achieve direct force control for decoupling the attitude and flight trajectory in the longitudinal flight. The nonlinear simulation using Canard Rotor/Wing (CRW) aircraft longitudinal model in fixed-wing mode demonstrates that this method can achieve two direct force control modes: pitch pointing and direct lift, whether the actuators are healthy or fail.