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Hussain, Mudassar,Waqas, Omer,Hassan, Usman,Loya, Asif,Akhtar, Noreen,Mushtaq, Sajid,Yusuf, Muhammad Aasim,Syed, Aamir Ali Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5
Background: There is growing evidence that there are differences in histological and genetic characteristics along with clinical behavior between right- and left-sided colon carcinomas. We have compared various parameters of the two types and assessed associations of the results with prognosis in patients in Pakistan. Materials and Methods: We reviewed 200 cases from our institutional database; 100 cases of right-sided and 100 cases of left-sided colon cancer. Parameters including age, gender, TNM stage, histological features and clinical outcome were analyzed. Results: The patients with right-sided colon cancer were significantly older as compared to their counterparts with left-sided cancer. They presented with a lumbar mass rather than symptoms of obstruction and perforation as seen in left-sided colon cancers, and the histology showed higher percentage of poorly differentiated tumors with advanced pT stage. Moreover, Crohn's-like reactions, intra tumoral lymphocyte responses and other poor prognostic factors like lymph vascular invasion and perineural invasion were more common in right-sided cancers. Conclusions: We found that right- and left-sided colon cancers are different from each other in terms of clinical presentation, histology and clinical behavior. Right-sided colon cancers are more aggressive and are associated with poorer clinical outcome as compared to left sided colon cancers in our population.
Akeem Yusuf Adesina,Muzafar Hussain,Abbas Saeed Hakeem,Abdul Samad Mohammed,Muhammad Ali Ehsan,Ahmad Sorour 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.9
This study investigates the influence of heating rate on the tribological and corrosion properties of 52100 bearing steelsamples consolidated via spark plasma sintering. The consolidation was conducted at different heating rates of 50, 100,200, 300, and 400 °C/min and the thermomechanical properties of the resulting samples were characterized. Ball-on-disctribological tests and electrochemical techniques were used to evaluate the wear and corrosion resistance, respectively. The results showed that an increased heating rate positively affects the thermomechanical and tribological properties of52100 bearing steel. The sintered samples exhibited a low coefficient of friction (between 0.4 and 0.56) and a low wear rate(between 1.4 and 1.8 × 10−6 mm3/Nm) at heating rates between 100 and 400 °C/min. Furthermore, the corrosion resistanceof the samples gradually drops above the heating rate of 100 °C/min. The samples can be ranked in the order of decreasingcorrosion resistance thus: 100 > 200 > 300 > 400 > 50 °C/min. The improved corrosion resistance of the sample sintered at100 °C/min can be attributed to its refined crystal size and high density.
Ghulam Hussain Jatoi,Guo Lihua,Yang Xiufen,Muswar Ali Gadhi,Azhar Uddin Keerio,Yusuf Ali Abdulle,Dewen Qiu 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.3
Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species (H2O2 and O2 -) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.
Jatoi, Ghulam Hussain,Lihua, Guo,Xiufen, Yang,Gadhi, Muswar Ali,Keerio, Azhar Uddin,Abdulle, Yusuf Ali,Qiu, Dewen The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.3
Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.
Deepak Kumar,Vipin Kumar Singh,Narayan Prasad Yadav,Sudeep Tandon,Karuna Shanker,Chandan Singh Chanotiya,Narendra Kumar,Puja Khare,Anirban Pal,Debabrata Chanda,Dharmendra Saikia,Yusuf Hussain,Abha Mee 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-
Gymnema sylvestre is an important medicinal plant, but a method for preparing standardized extractenriched with biomarkers is not available. The practiced crude hydro-alcoholic extracts of G. sylvestrecontained a few percentage of gymnemic acids (GAs). The enrichment of GAs in preprared extracts isessential from a pharmacology point of view. Presently, a scale-up process has been developed forpreparing G. sylvestre standardized extract (Gym-EEXT). Crude extract (Gym-Crude) was prepared in10-kg batches to obtain a yield of 268.1 mg/g, but GAs was merely 9 mg/g (GA-IV 6.7 mg/g, and GAXVII2.3 mg/g) in 30 % ethanol–water solvent at 50 C in 5 h. Further, Gym-Crude was processed throughpolymer-matrix-adsorption techniques for getting Gym-EEXT (150.9 mg/g) with improved percentage ofGAs (>10 %) comprised of deacylgymnemic acid (DGA: 8.9 mg/g), gymnemagenin (2.3 mg/g), GA-IV(78.6 mg/g), and GA-XVII (10.2 mg/g). For safety aspects, the pesticide residues, solvent residues, heavymetal, aflatoxins, and mutagenicity in Gym-EEXT were below the detection limits. Hence, Gym-EEXT wasenriched with GAs, and fully safe with compliance according to USP-561. An insignificant effect of Gym-EEXT on vitals of experimental mice was observed in acute and sub-acute oral-toxicityexperiments. Gym-EEXT significantly improved the blood glucose metabolism, and hence, it potentiallyreduces hyperglycaemia. Unique chromatographic protocols were developed for the isolation of biomarkerson a 1-kg scale from Gym-Crude with yield of gymnemagenin (5.5 %), and DGA (4.6 %). Finally, a novel,scientifically validated, and the scale-up process has been developed for the preparation of Gym-EEXTalong with an exclusive protocol for isolating its biomarkers.