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Yu‑hua Xie,Jiao Hu 한국유전학회 2020 Genes & Genomics Vol.42 No.11
Background Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulatorsincluding some lncRNAs, miRNAs, and proteins. Objective Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in gliomaprogression. Methods The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) wasapplied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19,miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIPassay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay wasused to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry,and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively. Results LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5pdecreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expressionof Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpressionof lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5psynergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasingMELK by performing as a sponge of miR-142-5p. Conclusions LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment.
Yu, Yong-Jiang,Li, Yu-Min,Hou, Xu-Dong,Guo, Chao,Cao, Nong,Jiao, Zuo-Yi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objective: Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasion and metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfected with human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction. Methods: The target gene TF was obtained from human placenta by nested PCR and introduced into the human gastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screened using G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and empty vector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show change in invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI double-staining was applied to detect apoptosis. Results: We generated the human gastric cancer cell line SGC7901/TF successfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereas caspase-3 activity and apoptosis rate were decreased in the experimental group. Conclusion: TF can enhance the invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition and apoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.
Effect of Thermal Annealing on the Optical Properties of In—Si—O/Ag/In—Si—O Multilayer
Yu, Jiao Long,Lee, Sang Yeol American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.5
<P>Low emissive transparent multilayers were fabricated with amorphous Si-doped indium oxide (ISO) transparent semiconductor and metallic Ag by Ag thickness. High transmittance in the visible range of the as-deposit ISO/Ag/ISO multilayer was verified in the experiment results, and the optical properties of an oxide-metal-oxide tri-layer were sensitive to the silver layer's thickness. After annealing, the transmittance in the visible range increased by over 10%, and low emissivity for infrared was maintained.</P>
Yu, Jiao Long,Lee, Sang Yeol The Korean Institute of Electrical and Electronic 2018 Transactions on Electrical and Electronic Material Vol.19 No.3
The optical and electrical properties of oxide-metal-oxide (OMO) multilayers composed of silver and either indium zinc oxide (IZO) or indium tin oxide (ITO), have been compared. Both the ITO and IZO-containing layers were deposited by sputtering at room temperature. Compared with the ITO layer, the IZO layer displayed a smoother, flatter surface. Due to the resulting smooth interface between IZO and Ag, the IZO OMO multilayers showed a high optical transmittance (ca. 90%) and high Hall mobility ($12.49-21.37cm^2v^{-1}s^{-1}$, depending on Ag thickness). This result suggests that IZO is a promising indium oxide-based material for OMO applications.
Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates
Jiao-Jiao Chen,Zhang Zhang,Yi Wang,Xiao-Long Yuan,Juan Wang,Yu-Ming Yang,Yuan Zheng 한국균학회 2023 Mycobiology Vol.51 No.1
Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzo- quinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3- hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM com- pared to the other two wood substrates. Similarly, the expression of geranylgeranyltransfer- ase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.
Jiao Long Yu,Sang Yeol Lee 한국전기전자재료학회 2018 Transactions on Electrical and Electronic Material Vol.19 No.3
The optical and electrical properties of oxide–metal–oxide (OMO) multilayers composed of silver and either indium zincoxide (IZO) or indium tin oxide (ITO), have been compared. Both the ITO and IZO-containing layers were deposited bysputtering at room temperature. Compared with the ITO layer, the IZO layer displayed a smoother, fl atter surface. Due to theresulting smooth interface between IZO and Ag, the IZO OMO multilayers showed a high optical transmittance (ca. 90%)and high Hall mobility (12.49–21.37 cm 2 v −1 s −1 , depending on Ag thickness). This result suggests that IZO is a promisingindium oxide-based material for OMO applications.
Effect of Carrier Diffusion on the Electrical Properties of Si–Zn–Sn–O/Ag/Si–Zn–Sn–O Multilayers
Jiao Long Yu,Sang Yeol Lee 한국전기전자재료학회 2018 Transactions on Electrical and Electronic Material Vol.19 No.3
Typically, a parallel circuit model is used to explain the electrical resistivity of oxide-metal-oxide (OMO) multilayers; such amodel treats the top, middle, and bottom sublayers independently. However, in the case of semiconductor–metal-semiconductormultilayer systems, this model is not applicable; according to the theory of metal-semiconductor contact, carrier diff usionarising from the diff erence in the work functions of the materials should also be considered. In this paper, we investigatethe eff ect of carrier diff usion on the electrical properties by changing the thickness of both the top and bottom oxide layers. We fi nd that adjusting the thickness of the oxide sublayers realizes direct control of the carrier density in OMO multilayers.
Effect of Thermal Annealing on the Electrical Properties of In-Si-O/Ag/In-Si-O Multilayer
Jiao Long Yu,이상렬 한국전기전자재료학회 2016 Transactions on Electrical and Electronic Material Vol.17 No.4
Transparent conductive multilayers have been fabricated using transparent amorphous Si doped indium oxide(ISO) semiconductors and metallic Ag of ISO/Ag/ISO. The resistivity of a multilayer is dependent on the middlelayer thickness of silver. The thickness of the Ag layer is fixed at 11 nm and takes into account cost and opticaltransmittance. As-deposited ISO/Ag (11 nm)/ISO multilayer shows a measured resistivity of 7.585×10-5 Ω cm. Aftera post annealing treatment of 400℃, the resistivity is reduced to 4.332×10-5 Ω cm. The reduction of resistivity shouldbe explained that the mobility of the multilayer increased due to the optimized crystalline, meanwhile, the Hallconcentration of the multilayer showed an obscure change because the carriers mainly come from the insert of the Aglayer.
Effect of Carrier Diffusion on the Electrical Properties of Si-Zn-Sn-O/Ag/Si-Zn-Sn-O Multilayers
Yu, Jiao Long,Lee, Sang Yeol The Korean Institute of Electrical and Electronic 2018 Transactions on Electrical and Electronic Material Vol.19 No.3
Typically, a parallel circuit model is used to explain the electrical resistivity of oxide-metal-oxide (OMO) multilayers; such a model treats the top, middle, and bottom sublayers independently. However, in the case of semiconductor-metal-semiconductor multilayer systems, this model is not applicable; according to the theory of metal-semiconductor contact, carrier diffusion arising from the difference in the work functions of the materials should also be considered. In this paper, we investigate the effect of carrier diffusion on the electrical properties by changing the thickness of both the top and bottom oxide layers. We find that adjusting the thickness of the oxide sublayers realizes direct control of the carrier density in OMO multilayers.