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Bu, Youngmin,Kwon, Sungeun,Kim, Yun Tai,Kim, Mi-Yeon,Choi, Hoyoung,Kim, Jae Goo,Jamarkattel-Pandit, Nirmala,Doré,, Sylvain,Kim, Sung-Hoon,Kim, Hocheol John Wiley Sons, Ltd. 2010 Phytotherapy research Vol.24 No.8
<P>HT008-1 is one of the prescriptions used in Traditional Korean Medicine for the treatment of mental and physical weakness. It is composed of Panax ginseng, Acanthopanax senticosus, Angelica sinensis and Scutellaria baicalensis, which have been reported to have various pharmacological effects on the central nervous system. The study investigated whether HT008-1 has neuroprotective effects in a focal cerebral ischemia rat model. Stroke was induced in rats by 2 h of middle cerebral artery occlusion (MCAo) followed by 22 h of reperfusion. HT008-1 (30, 100 and 300 mg/kg) and the component herbs (300 mg/kg) were administered orally twice at 0 and 2 h after ischemia. Oral administration of 300 mg/kg HT008-1 reduced brain infarction by 45.7%, prolonged the latency time by 24.8% in the rotarod test, and enhanced the score by 17.0% in the balance beam test. Only P. ginseng and S. baicalensis showed protective effects, and HT008-1 showed a greater effect than its component herbs. HT008-1 down-regulated the COX-2 and OX-42 expression in the penumbra region. In conclusion, the results show that HT008-1 may be effective in a rat stroke model by an antiinflammatory mechanism and may improve sensory-motor function by reducing damage in the cortex and caudoputamen. Copyright © 2010 John Wiley & Sons, Ltd.</P>
Telomerase induction in astrocytes of Sprague-Dawley rat after ischemic brain injury
Baek, Sunkyung,Bu, Youngmin,Kim, HwaSu,Kim, Hocheol WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Telomerase, a reverse transcriptase, consists of an RNA template and protein polymerase. This ribonucleoprotein protects the linearized chromosomal end region and elongates the telomere during chromosomal replication. Telomerase is not expressed in adult somatic cells but it shows high activity in most cells during embryonic development. We report, by RT-PCR and immunohistochemical results, that the induction of telomerase protein catalytic subunit (TERT) in transient middle cerebral artery occlusion induced brain injury. TERT mRNA emerged 24 h after ischemia. We examined which brain cell expressed TERT in the penumbra region of injured brain. The expression of TERT began from 24 h and remained until 5 days after ischemia. We identified that TERT was co-localized with the astrocyte marker, GFAP, at 3 days after ischemia. This is strong evidence that TERT is induced in astrocytes when the brain is damaged by ischemia, and that this enzyme may play an important role in ischemic brain injury.
KIM, Kanghyun,BU, Youngmin,JEONG, Seungil,LIM, Jongpil,KWON, Youngan,CHA, Dong Seok,KIM, Jinmo,JEON, Sora,EUN, Jaesoon,JEON, Hoon Japan Society for Bioscience, Biotechnology, and A 2006 Bioscience, Biotechnology, and Biochemistry Vol.70 No.8
<P><I>Abies koreana</I> Wilson (<I>A. koreana</I>) is a shrub or broadly pyramidal evergreen tree endemic in the mountainous regions of South Korea. We obtained the essential oil (EO) from alpine needle leaves of <I>A. koreana</I> by the supercritical fluid extraction (SFE) method. EO was analyzed by gas chromatography–mass spectrometry (GC–MS), and 68 compounds were identified constituting 95.66% of the oil. The major components were elemol (11.17%), terpinen-4-ol (9.77%), sabinene (8.86%), 10(15)-cadien-4-ol (7.16%), α-terpineol (6.13%), α-pinene (6.07%) and γ-terpinene (4.71%). To investigate the memory-enhancing effects, we conducted a passive avoidance test using a scopolamine (1 mg/kg, ip)-induced amnesia mouse model. A peritoneal injection of EO from <I>A. koreana</I> (100 mg/kg) showed a memory enhancing effect of 72.7% compared with the control. These results suggest that EO of <I>A. koreana</I> may be a useful therapeutic agent against such amnesia-inducing diseases as Alzheimer and vascular dementia.</P>
Effects of Korean Red Ginseng on Dry Mouth : A Randomized, Double-Blind, Placebo-Controlled Trial
Jae-Woo Park,Beom-Joon Lee,Youngmin Bu,Inkwon Yeo,Jinsung Kim,Bongha Ryu 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.3
Dry mouth is easily neglected if not associated with oral diseases. Consequently, xerostomatic patients often use unconventional therapies. In traditional Korean medicine, Korean red ginseng (KRG) has long been used to relieve dry mouth. However, no clinical trials have investigated whether KRG actually has an effect on dry mouth. This study was performed to evaluate the efficacy of KRG for dry mouth. We enrolled 100 volunteers with no obvious oral or salivary gland diseases and divided them into KRG and placebo groups. Each group was divided into six subgroups according to age and gender. The subjects received 6 g/day of KRG or placebo for 8 weeks. The dry mouth visual analog scale (VAS), salivary flow rate, and a dry mouth-related symptom questionnaire were evaluated at baseline and at 4 and 8 weeks. KRG treatment did not show any significant differences for any of the variables. However, KRG improved the dry mouth VAS at 4 weeks and dry mouthrelated symptoms at 8 weeks in women, but not in men. Subgroup analyses revealed that KRG markedly improved the dry mouth VAS in women of menopausal age (40 to 59 years) at 4 and 8 weeks. KRG may have beneficial effects for dry mouth in women, especially those of menopausal age, but not in men. Further investigation in post- and perimenopausal women is required to elaborate on these findings.
정혜진,Sunyoung Lim,In Sook Kim,Youngmin Bu,Hocheol Kim,김동현,유혜현 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.1
A new composition of standardized Scutellariae Radix extract (HPO12) was developed for treatment of Alzheimer’s disease. For the preclinical pharmacokinetic study of HPO12, a rapid, sensitive, and selective LCMS/MS method was developed and validated for the simultaneous determination of 4 bioactive compounds,baicalein, baicalin, wogonin, and wogonoside. After extraction with ethylacetate, chromatographic analysis was performed on a Thermo C18 column (150 mm × 2.1 mm, 3 μm) with a mobile phase consisting of 0.1%formic acid (A) and 0.1% formic acid in 95% acetonitrile (B) by using gradient elution at a flow rate of 250μL/min. Analytes introduced to a mass spectrometer were monitored by multiple reaction monitoring (MRM)in positive ion mode. Using 25 μL of plasma sample, the method was validated over the following concentration ranges: 25-5000 ng/mL for baicalein, 20-40000 ng/mL for baicalin, 1-1000 ng/mL for wogonin,and 5-10000 ng/mL for wogonoside. The intra- and inter-day precision and accuracy of the quality control samples at the 4 concentrations showed ≤ 13.7% relative standard deviation (RSD) and 86.6-105.5% accuracy. The method was successfully applied to determine the concentrations of baicalein, baicalin, wogonin, and wogonoside in rat plasma after intraperitoneal and oral administrations of HPO12.