Abnormal differentiation of regulatory T cells and Th17 cells induced by perinatal bisphenol A exposure in female offspring mice

You‑dan Dong,Liang Gao,Feng‑juan Wu,Ren Lin,Yuan Meng,Li‑hong Jia,Xiao‑fei Wang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Background Bisphenol A (BPA) is an environmental estrogen widely exposed to human beings, and there are more studies on its reproductive toxicity, endocrine disruption and neurobehavioral disorders. Recent few studies have found that BPA has immunotoxicity, and its mechanism is not clear. Therefore, the effects of BPA on immune system have attracted extensive attention. The aim of this study was to investigate the effect and mechanism of perinatal exposure to BPA on regulatory T cells (Treg) and Th17 cells in female offspring mice. Methods Twenty-one pregnant C57BL/6 mice were randomly divided into three groups: a control group, low-dose BPA (0.2 μg/mL) and high-dose BPA (2.0 μg/mL) exposure group. All received BPA exposure via drinking water from gestational day 6 to the end of lactation. Female offspring were fed a normal diet and drinking water for 1 month. The percentages of Treg and Th17 cells, the levels of Foxp3 and RORγt protein and IL-17 and TGF-β from spleen tissue or blood were measured in female offspring. Results The percentage of Treg cells and levels of Foxp3 protein decreased, while the percentage of Th17 cells and levels of RORγt protein increased, which showed a dose–effect relationship. The levels of serum TGF-β were significantly lower and the levels of serum IL-17 were statistically higher in BPA-exposed female offspring compared with controls (P < 0.05 or P < 0.01). But there were no statistical difference in the levels of serum TGF-β and IL-17 between 0.2 μg/mL and 2.0 μg/ mL BPA groups (P > 0.05). Conclusion BPA exposure during pregnancy and lactation could cause abnormal differentiation and function of Treg and Th17 cells in female offspring mice, which was associated with down-regulated Foxp3 and up-regulated RORγt protein, respectively. Our findings indicated that BPA exposure during early development may play an important role in the development of autoimmune diseases later.

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

Evolution of ALPPS: The Simpler, Safer and Effective One---TELPP

( Shu You Peng ),( Xu An Wang ),( Cong Yun Huang ),( You Yong Zhang ),( Jiang Tao Li ),( De Fei Hong ),( Xiu Jun Cai ),( Yi Fang Wang ),( Xiao Liang ),( Jian Wei Wang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The characteristic of associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) carries high mortality and morbidity. There is room for improvement. We suggest Terminal Branches Portal Vein Embolization (TBPVE) as a way to compart the liver. As a result, only a single surgical operation is required.This method is termed Terminal branches portal vein Embolization Liver Partition Planned hepatectomy (TELPP). Methods: Patients with unresectable primary or metastatic liver tumor were performed with TELPP. The procedure of TELPP was that in addition to PVE, embolization agent was infused to the terminal branches of portal vein of S5,S8 or S4. CT scan was taken one or two weeks later, and standard liver volume(SLV), FLR and FLR/SLV are calculated. Two weeks later when the FLR and liver function is appropriate, open or laparoscopic hepatectomy is performed. Results: The study included 11patients including hepatocellular carcinoma: n =8, intrahepatic cholangiocarcinoma: n = 1, hilarcholangiocarcinoma: n =1, colorectal liver metastasis: n =1. After a waiting period of 14 days, the volume of theFLR had increased from 382mlto 578ml, representing a median volume increase of 51% (range =32.5%-86.7%). Of the 11patients with hepatectomy, right hemihepatectomy (n=2), extended right hemihepatectomy (n=5), right trisecmentectomy(2), extended left hemihepatectomy (n=1) and left trisecmentectomy(1). No patient died, and no serve perioperative morbidity occurred. Conclusions: ALPPS and all modifications need two-stage operations with a high morbidity and mortality rate. It seems that TELPP is very promising. It has the merit of ALPPS as extraordinarily rapid increasement of FLRvolume, yet the morbidity and mortality is much lower, owing to the fact that unlike ALPPS, there is no two liver raw surfaces left behind in the abdominal cavity to produce bile leak, as only single surgical operation is required

Flexible Transmission Expansion Planning for Integrating Wind Power Based on Wind Power Distribution Characteristics

Wang, Jianxue,Wang, Ruogu,Zeng, Pingliang,You, Shutang,Li, Yunhao,Zhang, Yao The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

Traditional transmission planning usually caters for rated wind power output. Due to the low occurrence probability of nominal capacity of wind power and huge investment in transmission, these planning methods will leads to low utilization rates of transmission lines and poor economic efficiency. This paper provides a novel transmission expansion planning method for integrating large-scale wind power. The wind power distribution characteristics of large-scale wind power output and its impact on transmission planning are analyzed. Based on the wind power distribution characteristics, this paper proposes a flexible and economic transmission planning model which saves substantial transmission investment through spilling a small amount of peak output of wind power. A methodology based on Benders decomposition is used to solve the model. The applicability and effectiveness of the model and algorithm are verified through a numerical case.

Effect of Pruning Intensity on the Species Diversity of Arthropod Communities in Organic Tea Plantation

You-Ping Wang,Chao-Hong Ma,Ru-Hai Li 한국차학회 2015 한국차학회지 Vol.- No.S

Arthropod insects are important pests that feed on leaves and young shoots often causing 10 to 20% loss in tea yield. Evaluation of the effect of major cultural practice such as pruning on the ecological aspect of the arthropod insects and their natural enemies is essential to develop a sound IPM program for organic tea plantation. The impac of summer pruning in mid-May on the arthropod communities was investigated with 3 different pruning intensities,i.e. light, heavy, and no pruning, in Hubei organic tea plantation. Light summer pruning had significant impact on the species and number of tea bush insect pests and their natural enemies within 1 month after pruning, and it lasted by2 months. Heavy pruning had greater impacts. Light pruning postponed the first peak in occurrence of Empoasca vitis (Gothe) for 20 days and the peak of Acaphylla theae Watt for 30 days. Heavy pruning postponed the first peak of Empoasca vitis (Gothe) for 25 days, and completely avoided the damage on tea bush from Acaphylla theae Watt. difference in diversity indices and the ratio of ‘natural enemies/insect pests’ of the arthropod communities on tea bushes by 3 and 4 months after pruning, respectively. The results suggest that light pruning can be used to control insect pests in organic tea plantations with the effect lasting for two months. Heavy pruning had a better control of insect pests than light pruning, but it is hardly to recommend without confirming the balance among sustainability of tea bush vigor in growth and tea yield in the following year, population of natural enemies and the efficacy of insect pest control. The optimum intensity of pruning may differ by individual tea plantation as the age and vigor of tea bush, soil fertility and management of tea bush nutrition differ by each tea plantation.

An Inherent Zero-Voltage and Zero-Current-Switching Full-Bridge Converter with No Additional Auxiliary Circuits

Wang, Jianhua,Ji, Baojian,Wang, Hongbo,Chen, Naifu,You, Jun The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.3

An inherent zero-voltage and zero-current-switching phase-shifted full-bridge converter with reverse-blocking insulated-gate bipolar transistor (IGBT) or non-punch-through IGBT is proposed in this paper. This converter not only ensures that the switches in the lagging leg works at zero-current switching, but also minimizes circulating conduction loss without any additional auxiliary circuits. A 1.2 kW hardware prototype is designed, fabricated, and tested to verify the proposed topology. The control loop design procedures with small-signal models are also presented. A simple, low-cost, and robust democratic current-sharing circuit is also introduced and verified in this study. The proposed converter is a suitable alternative for compact, cost-effective applications with high-voltage input.

Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DAD-QTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS

Wang, Hong-Ping,Zhang, You-Bo,Yang, Xiu-Wei,Zhao, Da-Qing,Wang, Ying-Ping The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4

Background: Ginsenosides are the characteristic and principal components which manifest a variety of the biological and pharmacological activities of the roots and rhizomes of Panax ginseng (GRR). This study was carried out to qualitatively and quantitatively determine the ginsenosides in the cultivated and forest GRR. Methods: A rapid and sensitive ultra-high-performance liquid chromatography coupled with diode-array detector and quadrupole/time of flight tandem mass spectrometry (UPLC-DAD-QTOF-MS/MS) was applied to the qualitative analysis of ginsenosides and a 4000 QTRAP triple quadrupole tandem mass spectrometer (HPLC-ESI-MS) was applied to quantitative analysis of 19 ginsenosides. Results: In the qualitative analysis, all ingredients were separated in 10 min. A total of 131 ginsenosides were detected in cultivated and forest GRR. The method for the quantitative determination was validated for linearity, precision, and limits of detection and quantification. 19 representative ginsenosides were quantitated. The total content of all 19 ginsenosides in the forest GRR were much higher than those in the cultivated GRR, and were increased with the growing ages. Conclusion: This newly developed analysis method could be applied to the quality assessment of GRR as well as the distinction between cultivated and forest GRR.

An Equivalent Carrier-based Implementation of a Modified 24-Sector SVPWM Strategy for Asymmetrical Dual Stator Induction Machines

Wang, Kun,You, Xiaojie,Wang, Chenchen The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.4

A modified space vector pulse width modulation (SVPWM) strategy based on vector space decomposition and its equivalent carrier-based PWM realization are proposed in this paper, which is suitable for six-phase asymmetrical dual stator induction machines (DSIMs). A DSIM is composed of two sets of symmetrical three-phase stator windings spatially shifted by 30 electrical degrees and a squirrel-cage type rotor. The proposed SVPWM technique can reduce torque ripples and suppress the harmonic currents flowing in the stator windings. Above all, the equivalent relationship between the proposed SVPWM technique and the carrier-based PWM technique has been demonstrated, which allows for easy implementation by a digital signal processor (DSP). Simulation and experimental results, carried out separately on a simulation system and a 3.0 kW DSIM prototype test bench, are presented and discussed.

The Normality of Meromorphic Functions with Multiple Zeros and Poles Concerning Sharing Values

WANG, YOU-MING Department of Mathematics 2015 Kyungpook mathematical journal Vol.55 No.3

In this paper we study the problem of normal families of meromorphic functions concerning shared values. Let F be a family of meromorphic functions in the plane domain $D{\subseteq}{\mathbb{C}}$ and n, k be two positive integers such that $n{\geq}k+1$, and let a, b be two finite complex constants such that $a{\neq}0$. Suppose that (1) $f+a(f^{(k)})^n$ and $g+a(g^{(k)})^n$ share b in D for every pair of functions f, $g{\in}F$; (2) All zeros of f have multiplicity at least k + 2 and all poles of f have multiplicity at least 2 for each $f{\in}F$ in D; (3) Zeros of $f^{(k)}(z)$ are not the b points of f(z) for each $f{\in}F$ in D. Then F is normal in D. And some examples are provided to show the result is sharp.

EXISTENCE OF PERIODIC SOLUTIONS TO LIAPUNOV CHARACTERISTIC INDEX EQUATIONS

Wang, Han You,Ouyang, Jun,Yao, Zhuo 충청수학회 2006 충청수학회지 Vol.19 No.2

In this paper, a numerical programming for Liapunov index of differential equations with periodic coefficients depending on parameters is given. The associated equations are given at first, then existence of periodic solutions to the associated equations is proved in this paper. And we compute periodic solution u(t) of the associated equations. Finally, we give the error of approximate calculation.