http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Nohta, Hitoshi,Noma, Shunya,Ohkura, Yosuke,Yoo, Beong-Tae 충남대학교 약학대학 의약품개발연구소 1985 藥學論文集 Vol.1 No.-
A highly sensitive method for the assay of catechol-O-methyltransferase in erythrocytes is described, which employs high-performance liquid chromatography with fluorescence detection. A newly synthesized catechol compound, 2-(3,4-dihydroxyphenyl)naptho[1,2-d]-thiazole is used as a highly fluorogenic substrate for catechol-O-methyltransferase; the m-and p-methylated products formed enzymatically from the substrate under the optimum conditions, after extraction with n-hexane-chloroform, are separated by normal-phase chromatography on LiChrosorb Si 100. The limits of detection for m-and p-methylated products are 3 pmol per assay tube (60 fmol per injection volume of 20 ㎕) in each case. The ratio of m-and p-methylated products was 0.54. This method requires as little as 50㎕ of human erythrocytes.
Lee, Myung Koo,Hitoshi Nohta,Yosuke Ohkura,Yoo, Beong Tae 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-
Methods based on fluorimetry and high-performance liquid chromatography (h.p.l.c.) for higyly sensitive assay of aromatic L-amino acid decarboxylase are described. Dopamine formed enzymatically from a substrate, L-dihydroxyphenylalanine (_L-DOPA), after chromatography on a small column of a cation-exchanger, Toyopak SP, is converted to a fluorescent compound by reaction with 1,2-diphenylethylenediamine. The derivative is measured by direct spectrofluorimetry or by reversed-phase h.p.l.c. with fluorimetric detection. The limits of detection for dopamine formed enzymatically in the direct and h.p.l.c. methods are 15 and 1 pmol per assay tube, respectively. The enzyme in rat liver, kidney, brain, heart, adrenal medulla and serum can be precisely assayed.
Lee, Myung Koo,Hitoshi Nohta,Yosuke Ohkura,Yoo, Beong Tae 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-
A highly sensitive assay method for phenylethanolamine N-methyltransferase in rat adrenal medulla and brain is described which employs high-performance liquid chromatography with fluorescence detection. Eqiniphrine formed enzymatically from the substrate norepinephrine and isoproterenol (internal standare), after chromatography on a small cartridge of a cation exchanger, Tokopak SP, are converted into the corresponding florescent compounds by reaction with 1,2-diphenylethylenediamine, a selective fluorescence derivatization reagent for catechol compounds. The derivatives are separated by reversed-phase chromatography on TSK gel ODS-120T. The detection limit for epinephrine formed enzymatically is 0.66 pmol per assay tube.
Sensitive and Mild Fluorogenic Reagents for Biogenic Carboxylic Acids in HPLC
Ushijima, Tamano,Saito, Mikihiko,Sasamoto, Kazumi,Ohkura, Yosuke,Ueno, Keiyu 한국분석과학회 1995 분석과학 Vol.8 No.4
Five acid hydrazides as precolumn fluorescence derivatization reagents for carboxylic acids in HPLC, which have the benzofuran or benzothiazole moiety conjugated to a furan, thiophene or oxazoline ring, were synthesized and examined in view of reactivity, separability and sensitivity. Of these hydrazides, 2-(5-hydrazinocarbonyl-2-oxazolyl)-5,6-dimethoxybenzothiazole (BTOH) was most favorable. The detection limit of lauric acid as a model acid was 0.1 pmol per $10-{\mu}l$ injection volume at S/N=3, which was roughly equal to that of an analogous compound, 2-(5-hydrazinocarbonyl-2-furyl)-5,6-dimethoxybenzothiazole. The reagent allowed rapid assays of carboxylic acids ($C_{12:0}-C_{20:4}$) within 20 min with satisfactory scparability. The method was applied to the determination of fatty acids in human sera from healthy volunteers as well as from patients with diabetes or thyroid dysfunction.
Lee, Myung Koo,Hitoshi Nohta,Kenji Ohtsubo,Yoo, Beong Tae,Yosuke Ohkura 충남대학교 약학대학 의약품개발연구소 1987 藥學論文集 Vol.3 No.-
A simple and highly sensitive method for the determination of _L-3-dihydroxyphenylalanine (_L-DOPA) in human plasma and urine is described which employs high-performance liquid chromatography with fluorescence detection. After cation-exchange chromatography on a Toypak IC-SP S cartridge, _L-DOPA and α-methyldopa(an internal standard) in 300㎕ of plasma or 10㎕ of urine are converted into the corresponding fluorescent compounds by reaction with 1, 2-diphenylethylenediamine. These compounds are separated by reversed-phase chromatography on a TSK gel ODS-120T column with isocratic elution. The detection limit for _L-DOPA is 10 fmol in a 100-㎕ injection volume.