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Yoonkang Hur,Jeongyeo Lee,Hayoung Song,Yong Pyo Lim 한국식물생명공학회 2006 JOURNAL OF PLANT BIOTECHNOLOGY Vol.33 No.2
hinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetable crops in Korea and other East Asian countries. Cytosolic fructose-1,6-bisphosphatase (cytFBPase) is a key enzyme in sucrose biosynthesis, which controls the sucrose levels as well as the productivity of plants. The Chinese cabbage cytFBPase gene, BrFBPase, encodes the 340 amino acid polypeptide, giving a theoretical molecular weight of 37.2 kD and a isolectric point of 5.4. BrFBPase showed high sequence identity with Brassica homologs and its functional domains, such as F2,6P2 binding site or active site and F6P binding site, were highly conserved in diverse sources of organisms. Although the genome of Chinese cabbage seemed to be triplicated, BrFBPase appears to be a single copy gene. The expression of BrFBPase was examined at transcript and protein levels under various conditions. BrFBPase expression was observed only in photosynthetic source tissue, not in sink tissue. The expression was slightly higher during the day than at night, and it showed a diurnal cycle with circadian rhythmicity. Short-term exposure to low temperature inhibited the expression of the BrFBPase, while long-term exposure increased the expression, supporting that sugar levels are high in late autumn when temperatures are low.
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Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes
Myungjin Lee,Xiangshu Dong,Hayong Song,Ju Yeon Yang,Soyun Kim,YoonkangHur 한국유전학회 2020 Genes & Genomics Vol.42 No.10
Background Arabidopsis thaliana genome encodes ten DUF640 (short for domain of unknown function 640)/ALOG (short for Arabidopsis LSH1 and Oryza G1) proteins, also known as light-dependent short hypocotyl (LSH) proteins. While some of the LSH genes regulate organ boundary determination and shade avoidance response, the function of most of these genes remains largely unknown. Objective In this study, we aimed to characterize the function of AtLSH1 and AtLSH2 in Arabidopsis. Methods We overexpressed AtLSH1 and AtLSH2 (with or without the FLAG tag) in Arabidopsis Col-0 plants under the control of the 35S promoter. We also generated knockout or knockdown lines of these genes by miRNA-induced gene silencing (MIGS). We conducted intensive phenotypic analysis of these transgenic lines, and fnally performed RNA-seq analysis of two AtLSH2 overexpression (OX) lines. Results Although AtLSH1 and AtLSH2 amino acid sequences showed high similarly, AtLSH2-OX lines showed much higher levels of their transcripts than those of AtLSH1-OX lines. Additionally, overexpression of AtLSH1 and AtLSH2 greatly inhibited hypocotyl elongation in a light-independent manner, and reduced both vegetative and reproductive growth. However, knockout or knockdown of both these AtLSH genes did not afect plant phenotype. Gene Ontology (GO) analysis of diferentially expressed genes (DEGs) identifed by RNA-seq revealed enrichment of the GO term ‘response to stimulus’, included phytohormone-responsive genes; however, genes responsible for the abnormal phenotypes of AtLSH2-OX lines could not be identifed. Conclusion Although our data revealed no close association between light and phytohormone signaling components, overexpression of AtLSH1 and AtLSH2 greatly reduced vegetative and reproductive growth of Arabidopsis plants. This property could be used to generate new plants by regulating expression of AtLSH1 and AtLSH2.
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